SUFU Antibody (YA7147)
(Synonyms: UNQ650/PRO1280, SUFU, Suppressor of fused homolog, SUFUH)SUFU Antibody (YA7147) is a Mouse-derived and non-conjugated IgG1, Kappa monoclonal antibody, targeting to SUFU.
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Host:
Mouse
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Isotype:
IgG
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Application:
WB, IHC-P, IHC-F, ICC/IF
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Reactivity :
Human, African Green Monkey
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Formulation:
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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IHC-F
IHC-F: Immunohistochemistry-Frozen
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|---|
| Dilution Ratio | 1:500-2000 | 1:100-500 | 1:100-500 | 1:100-500 |
Product Details
SUFU Antibody (YA7147) is a Mouse-derived and non-conjugated IgG1, Kappa monoclonal antibody, targeting to SUFU.
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Host Mouse
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, African Green Monkey
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Calculated Molecular Weight Predicted band size: 53 or 106kDa;
Recombinant human SUFU
Endogenous
affinity purified by Protein G
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
SUFU is a Negative regulator in the hedgehog/smoothened signaling pathway. Down-regulates GLI1-mediated transactivation of target genes. Down-regulates GLI2-mediated transactivation of target genes. Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein. Negative regulator of beta-catenin signaling. Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full-length form of GLI3 (GLI3FL). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU-GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A). Required for normal embryonic development. Required for the proper formation of hair follicles and the control of epidermal differentiation[1][2][3][4][5][6][7][8][9][10][11].
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Subcellular Localization
Cytoplasm,Nucleus
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Expression
Tissue_Specificity: Ubiquitous in adult tissues. Detected in osteoblasts of the perichondrium in the developing limb of 12-week old embryos. Isoform 1 is detected in fetal brain, lung, kidney and testis. Isoform 2 is detected in fetal testis, and at much lower levels in fetal brain, lung and kidney -
Isoforms & Post-Translational Modification
Q9UMX1 has three isomers: Q9UMX1-1: 53947 Da (predicted); Q9UMX1-2: 47932 Da (predicted); Q9UMX1-3: 52977 Da (predicted).
Polyubiquitinated at Lys-257 by the SCF(FBXL17) complex, leading to its subsequent degradation and allowing the release of GLI1 for proper hedgehog/smoothened signal transduction (PubMed:27234298)丨Phosphorylation at Ser-342, Ser-346, Ser-352 and Thr-353 prevents ubiquitination by the SCF(FBXL17) complex -
Subunit
May form homodimers (PubMed:10564661)
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SwissProt ID
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Synonyms
UNQ650/PRO1280, SUFU, Suppressor of fused homolog, SUFUH
Documentation
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