CLEC4E Antibody (YA8006)(PBS only)

Cat. No.: HY-P88322A: Data Sheet User Guide for Antibodies
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CLEC4E Antibody (YA8006) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CLEC4E.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CLEC4E Antibody (YA8006) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to CLEC4E.

Host

Mouse

Molecular Weight

Predicted band size: 24.9 kDa

Species Reactivity

Human

SwissProt ID

Q9ULY5

Immunogen

Full length human recombinant protein of human CLEC4E produced in HEK293T cell.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:150
FC FC: Flow Cytometry	1:100

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Calcium-dependent lectin that acts as a pattern recognition receptor (PRR) of the innate immune system: recognizes damage-associated molecular patterns (DAMPs) of abnormal self and pathogen-associated molecular patterns (PAMPs) of bacteria and fungi (PubMed:18509109, PubMed:23602766). The PAMPs notably include mycobacterial trehalose 6,6'-dimycolate (TDM), a cell wall glycolipid with potent adjuvant immunomodulatory functions (PubMed:23602766, PubMed:24101491). Interacts with signaling adapter Fc receptor gamma chain/FCER1G to form a functional complex in myeloid cells (By similarity). Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to this receptor complex leads to phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FCER1G, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes (By similarity). Also recognizes alpha-mannose residues on pathogenic fungi of the genus Malassezia and mediates macrophage activation (By similarity). Through recognition of DAMPs released upon nonhomeostatic cell death, enables immune sensing of damaged self and promotes inflammatory cell infiltration into the damaged tissue (By similarity)

Subcellular Localization：Cell membrane,Cell projection, phagocytic cup

Expression：
Tissue_Specificity: Expressed in monocytes and macrophages

Isoforms & Post-Translational Modification：Q9ULY5: 219 amino acids, molecular weight 25073 Da.

Subunit：Monomer and homodimer (PubMed:18509109)

Synonyms

CLECSF9; MINCLE

Documentation

User Guide for Antibodies (1077 KB)