CPSF73 Antibody (YA6514)

Cat. No.: HY-P86821: Data Sheet COA
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CPSF73 Antibody (YA6514) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CPSF73.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

CPSF73 Antibody (YA6514) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CPSF73.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 77 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9UKF6

Gene ID

51692 [NCBI]

Immunogen

Recombinant protein within human CPSF73 aa 585-684/684.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. Has endonuclease activity, and functions as an mRNA 3'-end-processing endonuclease (PubMed:30507380). Also involved in the histone 3'-end pre-mRNA processing (PubMed:30507380). U7 snRNP-dependent protein that induces both the 3'-endoribonucleolytic cleavage of histone pre-mRNAs and acts as a 5' to 3' exonuclease for degrading the subsequent downstream cleavage product (DCP) of mature histone mRNAs. Cleavage occurs after the 5'-ACCCA-3' sequence in the histone pre-mRNA leaving a 3'hydroxyl group on the upstream fragment containing the stem loop (SL) and 5' phosphate on the downstream cleavage product (DCP) starting with CU nucleotides. The U7-dependent 5' to 3' exonuclease activity is processive and degrades the DCP RNA substrate even after complete removal of the U7-binding site. Binds to the downstream cleavage product (DCP) of histone pre-mRNAs and the cleaved DCP RNA substrate in a U7 snRNP dependent manner. Required for entering/progressing through S-phase of the cell cycle (PubMed:30507380). Required for the selective processing of microRNAs (miRNAs) during embryonic stem cell differentiation via its interaction with ISY1 (By similarity). Required for the biogenesis of all miRNAs from the pri-miR-17-92 primary transcript except miR-92a (By similarity). Only required for the biogenesis of miR-290 and miR-96 from the pri-miR-290-295 and pri-miR-96-183 primary transcripts, respectively (By similarity)

Subcellular Localization：Nucleus

Subunit：Component of the cleavage and polyadenylation specificity factor (CPSF) complex, composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1

Synonyms

Cleavage and polyadenylation specific factor 3 73kDa antibody; Cleavage and polyadenylation specificity factor 73 kDa subunit antibody; Cleavage and polyadenylation specificity factor subunit 3 antibody; CPSF 73 antibody; CPSF 73 kDa subunit antibody; CPSF antibody; CPSF-73 antibody; cpsf3 antibody; CPSF3_HUMAN antibody; CPSF73 antibody; Cleavage and polyadenylation specific factor 3 73kDa antibody; Cleavage and polyadenylation specificity factor 73 kDa subunit antibody; Cleavage and polyadenylation specificity factor subunit 3 antibody; CPSF 73 antibody; CPSF 73 kDa subunit antibody; CPSF antibody; CPSF-73 antibody; cpsf3 antibody; CPSF3_HUMAN antibody; CPSF73 antibody; mRNA 3''-end-processing endonuclease CPSF-73 antibody; YSH1 antibody;

Documentation

Select Batch:

Data Sheet (231 KB) SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)