2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Flow Cytometry Antibodies
  5. Ctip1 Antibody (YA489)

Ctip1 Antibody (YA489) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ctip1.

For research use only. We do not sell to patients.

Size Price Stock Quantity
Free Sample   Apply now  
10 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  
or Bulk Inquiry

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

  • Background

  • Documentation

  • References

Description

Ctip1 Antibody (YA489) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ctip1.
Host

Rabbit
Clonality

Monoclonal,Recombinant
Molecular Weight
Predicted band size: 91kDa;
Observed band size: 120kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human BCL 11A. The exact sequence is proprietary to MCE.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-2000
FC
FC: Flow Cytometry
1:50-100
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-200
Sensitivity Endogenous Purity affinity purified by Protein A
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 0.01M PBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
ICC

  • Immunocytochemistry analysis of C6 cells labeling Ctip1 with Ctip1 Antibody (HY-P80094) at 1:50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Ctip1 Antibody (HY-P80094) at 1:50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of C6 cells labeling Ctip1 with Ctip1 Antibody (HY-P80094) at 1:100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Ctip1 Antibody (HY-P80094) at 1:100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background
Function：Transcription factor (PubMed:16704730, PubMed:29606353). Associated with the BAF SWI/SNF chromatin remodeling complex (PubMed:23644491, PubMed:39607926). Binds to the 5'-TGACCA-3' sequence motif in regulatory regions of target genes, including a distal promoter of the HBG1 hemoglobin subunit gamma-1 gene (PubMed:29606353, PubMed:39423807). Involved in regulation of the developmental switch from gamma- to beta-globin, probably via direct repression of HBG1; hence indirectly repressing fetal hemoglobin (HbF) level (PubMed:26375765, PubMed:29606353, PubMed:39423807, PubMed:39607926). Involved in brain development (PubMed:27453576). May play a role in hematopoiesis (By similarity). Essential factor in lymphopoiesis required for B-cell formation in fetal liver (By similarity). May function as a modulator of the transcriptional repression activity of NR2F2 (By similarity)
Subcellular Localization：Cytoplasm; Nucleus; Chromosome; Nucleus matrix; Cytoplasm; Nucleus; Cytoplasm; Nucleus
Expression：
Tissue_specificity:Expressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development; Expressed in human brain, and in the plasmacytoid dendritic cell
Isoforms & Post-Translational Modification：Q9H165 has 5 isomers: Q9H165-1: 91197 Da (predicted); Q9H165-2: 83860 Da (predicted); Q9H165-3: 26866 Da (predicted); Q9H165-6: 87554 Da (predicted); Q9H165-8: 15720 Da (predicted).
Sumoylated with SUMO1
Subunit：Homotetrameric; self-associates via C2HC-type zinc finger domain (PubMed:39607926). Interacts with MTA2, a component of the nucleosome remodeling and deacetylase (NuRD) repressor complex (PubMed:39607926). Interacts (via its C2H2-type zinc finger domains 4, 5 and 6) with promoter region of gamma-globulin (PubMed:39423807). Interacts with NR2F1, PIAS3, NR2F2 and NR2F6 (By similarity). Isoform 1, isoform 2 and isoform 3 form homodimers and heterodimers (PubMed:27453576, PubMed:30250039). Isoform 2 interacts with TBR1 (PubMed:30250039)
Database

Entrez Gene: 53335 Human

SwissProt: Q9H165 Human

OMIM: 617101 Human

Documentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)
References

Ctip1 Antibody (YA489) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Ctip1 Antibody (YA489)
Cat. No.:
HY-P80094
Quantity:
MCE Japan Authorized Agent:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Country or Region *

Email Address *

     

Phone Number *

 

Organization Name *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (251 KB) Français - FR (251 KB) Deutsch - DE (251 KB) Norwegian - NO (251 KB) Español - ES (251 KB) Swedish - SV (251 KB) Italian - IT (251 KB) Korean - KR (251 KB) Portuguese - PT (251 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.