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Ctip1 Antibody (YA489)

(Synonyms: CTIP1, EVI9, KIAA1809, ZNF856, BCL11A, B-cell lymphoma/leukemia 11A, BCL-11A, B-cell CLL/lymphoma 11A, COUP-TF-interacting protein 1, Ecotropic viral integration site 9 protein homolog, Zinc finger protein 856, EVI-9)
Customer Review

Based on 1 Customer Validation

Ctip1 Antibody (YA489) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ctip1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, FC, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 0.01M PBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
ALL FC ICC
  • Experimental Validation Results for Ctip1 Antibody (YA489)
  • Experimental Validation Results for Ctip1 Antibody (YA489)
  • Experimental Validation Results for Ctip1 Antibody (YA489)
  • Ctip1 Antibody (YA489) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-2000 1:50-100 1:50-200

Product Details

Description

Ctip1 Antibody (YA489) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ctip1.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 120kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 91kDa
Species Reactivity Database

Entrez Gene: 53335 Human

SwissProt: Q9H165 Human

Immunogen

A synthesized peptide derived from human BCL 11A. The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified by Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M PBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Ctip1 Antibody (YA489)
    Flow cytometric analysis of 1X106 Jurkat cells labeling Ctip1 Antibody (HY-P80094, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Ctip1 Antibody (YA489)
    Immunocytochemistry analysis of C6 cells labeling Ctip1 with Ctip1 Antibody (HY-P80094) at 1:50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Ctip1 Antibody (HY-P80094) at 1:50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Ctip1 Antibody (YA489)
    Immunocytochemistry analysis of C6 cells labeling Ctip1 with Ctip1 Antibody (HY-P80094) at 1:100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Ctip1 Antibody (HY-P80094) at 1:100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Transcription factor (PubMed:16704730, PubMed:29606353). Associated with the BAF SWI/SNF chromatin remodeling complex (PubMed:23644491, PubMed:39607926). Binds to the 5'-TGACCA-3' sequence motif in regulatory regions of target genes, including a distal promoter of the HBG1 hemoglobin subunit gamma-1 gene (PubMed:29606353, PubMed:39423807). Involved in regulation of the developmental switch from gamma- to beta-globin, probably via direct repression of HBG1; hence indirectly repressing fetal hemoglobin (HbF) level (PubMed:26375765, PubMed:29606353, PubMed:39423807, PubMed:39607926). Involved in brain development (PubMed:27453576). May play a role in hematopoiesis (By similarity). Essential factor in lymphopoiesis required for B-cell formation in fetal liver (By similarity). May function as a modulator of the transcriptional repression activity of NR2F2 (By similarity)

  • Subcellular Localization

    Cytoplasm; Nucleus; Chromosome; Nucleus matrix; Cytoplasm; Nucleus; Cytoplasm; Nucleus

  • Expression


    Tissue_specificity:Expressed at high levels in brain, spleen thymus, bone marrow and testis. Expressed in CD34-positive myeloid precursor cells, B-cells, monocytes and megakaryocytes. Expression is tightly regulated during B-cell development; Expressed in human brain, and in the plasmacytoid dendritic cell

  • Isoforms & Post-Translational Modification

    Q9H165 has 5 isomers: Q9H165-1: 91197 Da (predicted); Q9H165-2: 83860 Da (predicted); Q9H165-3: 26866 Da (predicted); Q9H165-6: 87554 Da (predicted); Q9H165-8: 15720 Da (predicted).
    Sumoylated with SUMO1

  • Subunit

    Homotetrameric; self-associates via C2HC-type zinc finger domain (PubMed:39607926). Interacts with MTA2, a component of the nucleosome remodeling and deacetylase (NuRD) repressor complex (PubMed:39607926). Interacts (via its C2H2-type zinc finger domains 4, 5 and 6) with promoter region of gamma-globulin (PubMed:39423807). Interacts with NR2F1, PIAS3, NR2F2 and NR2F6 (By similarity). Isoform 1, isoform 2 and isoform 3 form homodimers and heterodimers (PubMed:27453576, PubMed:30250039). Isoform 2 interacts with TBR1 (PubMed:30250039)

  • SwissProt ID

    Q9H165

  • Gene ID
    53335 [NCBI]
  • Synonyms

    CTIP1, EVI9, KIAA1809, ZNF856, BCL11A, B-cell lymphoma/leukemia 11A, BCL-11A, B-cell CLL/lymphoma 11A, COUP-TF-interacting protein 1, Ecotropic viral integration site 9 protein homolog, Zinc finger protein 856, EVI-9

Ctip1 Antibody (YA489) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Jurkat cells labeling Ctip1 Antibody (HY-P80094, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling Ctip1 with Ctip1 Antibody (HY-P80094) at 1:50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Ctip1 Antibody (HY-P80094) at 1:50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling Ctip1 with Ctip1 Antibody (HY-P80094) at 1:100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Ctip1 Antibody (HY-P80094) at 1:100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Ctip1 Antibody (YA489) Product Image
    1/16
  • Experimental Validation Results for Ctip1 Antibody (YA489)
  • Experimental Validation Results for Ctip1 Antibody (YA489)
  • Experimental Validation Results for Ctip1 Antibody (YA489)
  • Ctip1 Antibody (YA489) Product Image
  • Experimental Validation Results for Ctip1 Antibody (YA489)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 Jurkat cells labeling Ctip1 Antibody (HY-P80094, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Ctip1 Antibody (YA489)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling Ctip1 with Ctip1 Antibody (HY-P80094) at 1:50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Ctip1 Antibody (HY-P80094) at 1:50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Ctip1 Antibody (YA489)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of C6 cells labeling Ctip1 with Ctip1 Antibody (HY-P80094) at 1:100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Ctip1 Antibody (HY-P80094) at 1:100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Ctip1 Antibody (YA489)
  • Experimental Validation Results for Ctip1 Antibody (YA489)
  • Experimental Validation Results for Ctip1 Antibody (YA489)
  • Ctip1 Antibody (YA489) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 Jurkat cells labeling Ctip1 Antibody (HY-P80094, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Ctip1 Antibody (YA489) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of C6 cells labeling Ctip1 with Ctip1 Antibody (HY-P80094) at 1:50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Ctip1 Antibody (HY-P80094) at 1:50 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Ctip1 Antibody (YA489) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of C6 cells labeling Ctip1 with Ctip1 Antibody (HY-P80094) at 1:100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Ctip1 Antibody (HY-P80094) at 1:100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L（HY-P8002, Green） was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Ctip1 Antibody (YA489) Product Image FC
  • Ctip1 Antibody (YA489) Product Image ICC
  • Ctip1 Antibody (YA489) Product Image ICC