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CD62P Antibody (YA3274)

(Synonyms: SELP; GMRP; GRMP; P-selectin; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD62P)
Customer Review

Based on 1 Customer Validation

CD62P Antibody (YA3274) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD62P.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P

  • Reactivity :

    Human

  • Formulation:

    Supplied in Rabbit IgG in PBS(pH 7.4), 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for CD62P Antibody (YA3274)
  • Experimental Validation Results for CD62P Antibody (YA3274)
  • Experimental Validation Results for CD62P Antibody (YA3274)
  • CD62P Antibody (YA3274) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:1000-1:2000 1:50-1:100

Product Details

Description

CD62P Antibody (YA3274) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CD62P.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 105/140 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 91 kDa
Species Reactivity Database

Entrez Gene: 6403 Human

SwissProt: P16109 Human

Immunogen

A synthesized peptide derived from human CD62P aa780-830/830.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3105033

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in Rabbit IgG in PBS(pH 7.4), 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD62P Antibody (YA3274)
    Western blot analysis of extracts from HUVEC(lane 2(20ug) and HUVEC(lane 3(40ug) using CD62P Antibody (HY-P83529) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD62P Antibody (YA3274)
    Immunohistochemical analysis of paraffin-embedded human placenta using CD62P antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83529, 1/100) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD62P Antibody (YA3274)
    Immunohistochemical analysis of paraffin-embedded human bone marrow using CD62P antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83529, 1/100) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    CD62P (P-selectin) belongs to the selectin family and is an adhesion molecule rapidly exposed on the cell surface following the activation of platelets and endothelial cells; it serves as a key marker for detecting platelet activation, platelet-leukocyte interactions, and inflammatory thrombosis.

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:P-selectin is stored in the α-granules of platelets and the Weibel-Palladian bodies of endothelial cells. When cells are activated by agonists, P-selectin is rapidly transported to the cell surface.

  • Subunit

    Interacts with SNX17. Interacts with SELPLG/PSGL1 and PODXL2 and mediates neutrophil adhesion and leukocyte rolling. This interaction requires the sialyl-Lewis X epitope of SELPLG and PODXL2, and specific tyrosine sulfation on SELPLG. Interacts (via C-type lectin domain) with alpha-IIb/beta3 integrin ITGA2B:ITGB3 and alpha-V/beta-3 integrin ITGAV:ITGB3 (PubMed:37184585). Interacts with alpha5/beta1 integrin ITGA5:ITGB1 and alpha4/beta1 integrin ITGA4:ITGB (PubMed:37184585)

  • SwissProt ID

    P16109

  • Gene ID
    6403 [NCBI]
  • Synonyms

    SELP; GMRP; GRMP; P-selectin; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD62P

  • Research Field

    Cardiovascular

References

[1]. Takada YK, et al. The C-type lectin domain of CD62P (P-selectin) functions as an integrin ligand. Life Sci Alliance. 2023 Jul;6(7):. [Content Brief]

CD62P Antibody (YA3274) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HUVEC(lane 2(20ug) and HUVEC(lane 3(40ug) using CD62P Antibody (HY-P83529) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta using CD62P antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83529, 1/100) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bone marrow using CD62P antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83529, 1/100) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD62P Antibody (YA3274) Product Image
    1/16
  • Experimental Validation Results for CD62P Antibody (YA3274)
  • Experimental Validation Results for CD62P Antibody (YA3274)
  • Experimental Validation Results for CD62P Antibody (YA3274)
  • CD62P Antibody (YA3274) Product Image
  • Experimental Validation Results for CD62P Antibody (YA3274)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HUVEC(lane 2(20ug) and HUVEC(lane 3(40ug) using CD62P Antibody (HY-P83529) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for CD62P Antibody (YA3274)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta using CD62P antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83529, 1/100) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD62P Antibody (YA3274)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human bone marrow using CD62P antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83529, 1/100) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for CD62P Antibody (YA3274)
  • Experimental Validation Results for CD62P Antibody (YA3274)
  • Experimental Validation Results for CD62P Antibody (YA3274)
  • CD62P Antibody (YA3274) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HUVEC(lane 2(20ug) and HUVEC(lane 3(40ug) using CD62P Antibody (HY-P83529) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • CD62P Antibody (YA3274) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta using CD62P antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83529, 1/100) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD62P Antibody (YA3274) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human bone marrow using CD62P antibody. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) for 14 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with TBST, and then probed with the primary antibody (HY-P83529, 1/100) for 1 hour at room temperature. The detection was performed using Polymer HRP-conjugated Goat Anti-Mouse/Rabbit lgG(H&L) secondary antibody (HY-P83652). DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • CD62P Antibody (YA3274) Product Image WB
  • CD62P Antibody (YA3274) Product Image IHC-P
  • CD62P Antibody (YA3274) Product Image IHC-P