VRK1 Antibody (YA7880)(PBS only)

Cat. No.: HY-P88196A: Data Sheet User Guide for Antibodies
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VRK1 Antibody (YA7880) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to VRK1.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

VRK1 Antibody (YA7880) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to VRK1.

Host

Mouse

Molecular Weight

Predicted band size: 45.3 kDa

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q99986

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-230 of human VRK1 produced in E.coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000

Purity	Affinity purified	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Serine/threonine kinase involved in the regulation of key cellular processes including the cell cycle, nuclear condensation, transcription regulation, and DNA damage response (PubMed:14645249, PubMed:18617507, PubMed:19103756, PubMed:33076429). Controls chromatin organization and remodeling by mediating phosphorylation of histone H3 on 'Thr-4' and histone H2AX (H2aXT4ph) (PubMed:31527692, PubMed:37179361). It also phosphorylates KAT5 in response to DNA damage, promoting KAT5 association with chromatin and histone acetyltransferase activity (PubMed:33076429). Is involved in the regulation of cell cycle progression of neural progenitors, and is required for proper cortical neuronal migration (By similarity). Is involved in neurite elongation and branching in motor neurons, and has an essential role in Cajal bodies assembly, acting through COIL phosphorylation and the control of coilin degradation (PubMed:21920476, PubMed:31090908, PubMed:31527692). Involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, it is required to induce Golgi fragmentation (PubMed:19103756). Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm (PubMed:16495336). Phosphorylates TP53BP1 and p53/TP53 on 'Thr-18', preventing the interaction between p53/TP53 and MDM2 (PubMed:10951572, PubMed:31527692). Phosphorylates ATF2 which activates its transcriptional activity (PubMed:15105425). Phosphorylates JUN (PubMed:31527692)

Subcellular Localization：Nucleus,Cytoplasm,Nucleus, Cajal body

Expression：
Tissue_Specificity: Widely expressed. Highly expressed in fetal liver, testis and thymus

Isoforms & Post-Translational Modification：Q99986: 396 amino acids, molecular weight 45476 Da.

Subunit：Interacts with HDAC1, KAT2B, SETDB1, KDM3A and KDM4A (PubMed:37179361)

Synonyms

PCH1; PCH1A

Documentation

User Guide for Antibodies (1077 KB)