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Cytokeratin 18 Antibody

(Synonyms: K1C18_HUMAN; Keratin, type I cytoskeletal 18; KRT18; CYK18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18 (CK-18); Keratin-18 (K18); PIG46; Keratin 18; )
1 Cited Publications
Customer Review

Based on 1 publication(s) in Google Scholar

Cytokeratin 18 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Cytokeratin 18.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ELISA, IHC-P, IHC-F, FC, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Cytokeratin 18 Antibody Product Image
ALL WB IHC-P
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Cytokeratin 18 Antibody Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

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Applications

Application
WB Info
WB: Western Blot
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
FC Info
FC: Flow Cytometry
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-2000 1:5000-10000 1:100-500 1:100-500 1μg/test 1:100 1:100-500

Product Details

Description

Cytokeratin 18 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Cytokeratin 18.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse, Rat Predicted Reactivity: Dog,Pig,Cow,Horse,Rabbit
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 48 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 48 kDa
Species Reactivity Database
Immunogen

KLH conjugated synthetic peptide derived from human CK18: 53-150/430

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103259

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Cytokeratin 18 Antibody
    Western blot analysis of extracts from M-intestine (lane 1) and M-lung (lane 2) using CK18 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Cytokeratin 18 Antibody
    Immunohistochemical analysis of paraffin-embedded human liver tissue using Cytokeratin 18 Antibody (HY-P81181, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cytokeratin 18 Antibody
    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Cytokeratin 18 Antibody (HY-P81181, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cytokeratin 18 Antibody
    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Cytokeratin 18 Antibody (HY-P81181, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cytokeratin 18 Antibody
    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Cytokeratin 18 Antibody (HY-P81181, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cytokeratin 18 Antibody
    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Cytokeratin 18 Antibody (HY-P81181, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Cytokeratin 18 Antibody
    Immunohistochemical analysis of paraffin-embedded mouse duodenum tissue using Cytokeratin 18 Antibody (HY-P81181, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection

  • Subcellular Localization

    Nucleus matrix; Cytoplasm, perinuclear region; Nucleus, nucleolus; Cytoplasm

  • Expression


    Tissue_specificity:It is expressed in the colon, placenta, and liver, with very weak expression at the external os of the cervix. Increased expression has been observed in breast cancer lymph nodes.

    Induction:By IL6/interleukin-6

  • Subunit

    Heterotetramer of two type I and two type II keratins. KRT18 associates with KRT8 (PubMed:10852826, PubMed:24940650). Interacts with PLEC isoform 1C, when in a heterodimer with KRT8 (PubMed:24940650).

  • SwissProt ID

    P05783

  • Gene ID
    3875 [NCBI]
  • Synonyms

    K1C18_HUMAN; Keratin, type I cytoskeletal 18; KRT18; CYK18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18 (CK-18); Keratin-18 (K18); PIG46; Keratin 18;

Cytokeratin 18 Antibody Related Classifications

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    Cytokeratin 18 Antibody purchased from MedChemExpress. Usage Cited in:

    MedchemExpress Validation
     Western blot analysis of extracts from M-intestine (lane 1) and M-lung (lane 2) using CK18 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • 1/16

    Cytokeratin 18 Antibody purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human liver tissue using Cytokeratin 18 Antibody (HY-P81181, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    Cytokeratin 18 Antibody purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Cytokeratin 18 Antibody (HY-P81181, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    Cytokeratin 18 Antibody purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Cytokeratin 18 Antibody (HY-P81181, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    Cytokeratin 18 Antibody purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Cytokeratin 18 Antibody (HY-P81181, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    Cytokeratin 18 Antibody purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Cytokeratin 18 Antibody (HY-P81181, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    Cytokeratin 18 Antibody purchased from MedChemExpress. Usage Cited in:

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse duodenum tissue using Cytokeratin 18 Antibody (HY-P81181, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cytokeratin 18 Antibody Product Image
    1/16
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Experimental Validation Results for Cytokeratin 18 Antibody
  • Cytokeratin 18 Antibody Product Image
  • Cytokeratin 18 Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from M-intestine (lane 1) and M-lung (lane 2) using CK18 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • Cytokeratin 18 Antibody Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human liver tissue using Cytokeratin 18 Antibody (HY-P81181, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cytokeratin 18 Antibody Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human adrenal gland tissue using Cytokeratin 18 Antibody (HY-P81181, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cytokeratin 18 Antibody Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue using Cytokeratin 18 Antibody (HY-P81181, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cytokeratin 18 Antibody Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using Cytokeratin 18 Antibody (HY-P81181, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cytokeratin 18 Antibody Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using Cytokeratin 18 Antibody (HY-P81181, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cytokeratin 18 Antibody Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse duodenum tissue using Cytokeratin 18 Antibody (HY-P81181, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Cytokeratin 18 Antibody Product Image WB
  • Cytokeratin 18 Antibody Product Image IHC-P
  • Cytokeratin 18 Antibody Product Image IHC-P
  • Cytokeratin 18 Antibody Product Image IHC-P
  • Cytokeratin 18 Antibody Product Image IHC-P
  • Cytokeratin 18 Antibody Product Image IHC-P
  • Cytokeratin 18 Antibody Product Image IHC-P