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SLC22A12 Antibody (YA4439)

(Synonyms: RST; OAT4L; URAT1)
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Based on 1 Customer Validation

SLC22A12 Antibody (YA4439) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SLC22A12.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P, FC, ELISA

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for SLC22A12 Antibody (YA4439)
  • SLC22A12 Antibody (YA4439) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

SLC22A12 Antibody (YA4439) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SLC22A12.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 60 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 60 kDa
Immunogen

Purified recombinant fragment of human SLC22A12 (AA: 30-145) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718975

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for SLC22A12 Antibody (YA4439)
    Western blot analysis of extracts from MCF-7 (lane 2(20μg), HeLa (lane 3(20μg), HepG2 (lane 4(20μg) and 293T (lane 5(20μg) using SLC22A12 Antibody (HY-P84742) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

  • Function

    SLC22A12 is an Electroneutral antiporter that translocates urate across the apical membrane of proximal tubular cells in exchange for monovalent organic or inorganic anions. Involved in renal reabsorption of urate and helps maintaining blood levels of uric acid. Mediates urate uptake by an exchange witH2O2ganic anions such as (S)-lactate and nicotinate, and inorganic anion Cl(-). Other inorganic anions such as Br(-), I(-) and NO3(-) may also act as counteranions that exchange for urate. Also mediates orotate tubular uptake coupled with nicotinate efflux and to a lesser extent with lactate efflux, therefore displaying a potential role in orotate renal reabsorption. Orotate transport is Cl(-)-dependent[1][2][3][4][5].

  • Subcellular Localization

    Apical cell membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:Detected in kidney (at protein level) . Detected in human kidney. Detected in epithelial cells of proximal tubules in renal cortex

  • Isoforms & Post-Translational Modification

    Q96S37 has 4 isomers: Q96S37-1: 59630 Da (predicted); Q96S37-2: 47648 Da (predicted); Q96S37-3: 35570 Da (predicted); Q96S37-4: 56094 Da (predicted).
    N-glycosylated

  • Subunit

    Interacts with PDZK1

  • SwissProt ID

    Q96S37

  • Gene ID
    116085 [NCBI]
  • Synonyms

    RST; OAT4L; URAT1

SLC22A12 Antibody (YA4439) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane 2(20μg), HeLa (lane 3(20μg), HepG2 (lane 4(20μg) and 293T (lane 5(20μg) using SLC22A12 Antibody (HY-P84742) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • SLC22A12 Antibody (YA4439) Product Image
    1/16
  • Experimental Validation Results for SLC22A12 Antibody (YA4439)
  • SLC22A12 Antibody (YA4439) Product Image
  • Experimental Validation Results for SLC22A12 Antibody (YA4439)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from MCF-7 (lane 2(20μg), HeLa (lane 3(20μg), HepG2 (lane 4(20μg) and 293T (lane 5(20μg) using SLC22A12 Antibody (HY-P84742) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for SLC22A12 Antibody (YA4439)
  • SLC22A12 Antibody (YA4439) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from MCF-7 (lane 2(20μg), HeLa (lane 3(20μg), HepG2 (lane 4(20μg) and 293T (lane 5(20μg) using SLC22A12 Antibody (HY-P84742) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • SLC22A12 Antibody (YA4439) Product Image WB