SLC22A12 Antibody (YA4439)

Cat. No.: HY-P84742: Data Sheet COA
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SLC22A12 Antibody (YA4439) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SLC22A12.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

SLC22A12 Antibody (YA4439) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to SLC22A12.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 60 kDa;

Observed band size: 60 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q96S37

Gene ID

116085 [NCBI]

Immunogen

Purified recombinant fragment of human SLC22A12 (AA: 30-145) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from MCF-7 (lane 2(20μg), HeLa (lane 3(20μg), HepG2 (lane 4(20μg) and 293T (lane 5(20μg) using SLC22A12 Antibody (HY-P84742) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Electroneutral antiporter that translocates urate across the apical membrane of proximal tubular cells in exchange for monovalent organic or inorganic anions (PubMed:12024214, PubMed:22194875, PubMed:35144162, PubMed:35462902). Involved in renal reabsorption of urate and helps maintaining blood levels of uric acid (PubMed:12024214, PubMed:22194875). Mediates urate uptake by an exchange witH2O2ganic anions such as (S)-lactate and nicotinate, and inorganic anion Cl(-) (PubMed:12024214). Other inorganic anions such as Br(-), I(-) and NO3(-) may also act as counteranions that exchange for urate (PubMed:12024214). Also mediates orotate tubular uptake coupled with nicotinate efflux and to a lesser extent with lactate efflux, therefore displaying a potential role in orotate renal reabsorption (PubMed:21350910). Orotate transport is Cl(-)-dependent (PubMed:21350910)

Subcellular Localization：Apical cell membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:Detected in kidney (at protein level) . Detected in human kidney. Detected in epithelial cells of proximal tubules in renal cortex

Isoforms & Post-Translational Modification：Q96S37 has 4 isomers: Q96S37-1: 59630 Da (predicted); Q96S37-2: 47648 Da (predicted); Q96S37-3: 35570 Da (predicted); Q96S37-4: 56094 Da (predicted).
N-glycosylated

Subunit：Interacts with PDZK1

RRID

AB_3718975

Synonyms

RST; OAT4L; URAT1

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)