ketohexokinase Antibody (YA6856)
(Synonyms: EC 2.7.1.3 antibody; Fructokinase antibody; Hepatic fructokinase antibody; Ketohexokinase antibody; Ketohexokinase isoform a antibody; KHK antibody; KHK_HUMAN antibody; )ketohexokinase Antibody (YA6856) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ketohexokinase.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IHC-P, FC
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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|---|---|---|---|---|
| Dilution Ratio | 1:2000 | 1:100 | 1:500-1:2000 | 1:1000 |
Product Details
ketohexokinase Antibody (YA6856) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to ketohexokinase.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 33 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 33 kDa
Recombinant protein within human ketohexokinase aa 1-298 / 298.
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Ketohexokinase (KHK), a member of the ribokinase superfamily of kinases, catalyzes the conversion of fructose to fructose-1-phosphate in an ATP- and K+- dependent manner. This reaction is the rate-limiting first step in the fructose metabolic pathway. The Ketohexokinase gene gives rise to two isoforms, KHK-A and KHK-C, through alternative splicing. KHK-C, mainly expressed in the liver and small intestine, is responsible for the majority of fructose metabolism in these tissues, playing a key role in normal fructose-induced lipogenesis and other metabolic processes. In contrast, KHK-A, ubiquitously expressed in most tissues, has a relatively low enzymatic activity for fructose phosphorylation under physiological conditions. Dysregulation of Ketohexokinase activity, especially over-activation in the context of high fructose intake, is closely associated with a variety of metabolic disorders, including obesity, diabetes, non-alcoholic fatty liver disease (NAFLD), and non-alcoholic steatohepatitis (NASH) [1][2][3].
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Expression
Tissue_Specificity: Most abundant in liver, kidney, gut, spleen and pancreas. Low levels also found in adrenal, muscle, brain and eye -
Isoforms & Post-Translational Modification
P50053 has two isomers: P50053-1: 32523 Da (predicted); P50053-2: 32730 Da (predicted).
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Subunit
Homodimer
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SwissProt ID
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Synonyms
EC 2.7.1.3 antibody; Fructokinase antibody; Hepatic fructokinase antibody; Ketohexokinase antibody; Ketohexokinase isoform a antibody; KHK antibody; KHK_HUMAN antibody;
Documentation
References
[1]. Kim J, et al. Ketohexokinase-A acts as a nuclear protein kinase that mediates fructose-induced metastasis in breast cancer. Nat Commun. 2020 Oct 28;11(1):5436. [Content Brief]
[2]. Gutierrez JA, et al. Pharmacologic inhibition of ketohexokinase prevents fructose-induced metabolic dysfunction. Mol Metab. 2021 Jun;48:101196. [Content Brief]
[3]. Diggle CP, et al. Ketohexokinase: expression and localization of the principal fructose-metabolizing enzyme. J Histochem Cytochem. 2009 Aug;57(8):763-74. [Content Brief]
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