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Glypican 3 Antibody (YA5609)

(Synonyms: DGSX; Glypican proteoglycan 3; Glypican-3 [Precursor]; Gpc3; GPC3_HUMAN; GTR2 2; GTR2-2; Heparan sulphate proteoglycan; Intestinal protein OCI 5; Intestinal protein OCI-5; MXR7; OCI 5; OCI-5; OCI5; SDYS; Secreted glypican-3; SGB; SGBS; SGBS1)
Customer Review

Based on 1 Customer Validation

Glypican 3 Antibody (YA5609) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Glypican 3.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    IHC-P, ICC/IF, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Glypican 3 Antibody (YA5609)
  • Experimental Validation Results for Glypican 3 Antibody (YA5609)
  • Glypican 3 Antibody (YA5609) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1000 1:100-500 1:1000-5000

Product Details

Description

Glypican 3 Antibody (YA5609) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Glypican 3.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 58-70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 66 kDa
Immunogen

Synthesized peptide derived from human Glypican-3(GPC3) AA range: 400-500

Purification

Protein G

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Glypican 3 Antibody (YA5609)
    Immunocytochemistry analysis of Hela cells labeling Glypican 3 with Glypican 3 Antibody (HY-P85917) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Glypican 3 Antibody (HY-P85917) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Glypican 3 Antibody (YA5609)
    Immunocytochemistry analysis of HepG2 cells labeling Glypican 3 with Glypican 3 Antibody (HY-P85917) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Glypican 3 Antibody (HY-P85917) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Glypican 3 cell surface proteoglycan. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression. Inhibits the dipeptidyl peptidase activity of DPP4. Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4. Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis. Required for coronary vascular development. Plays a role in regulating cell movements during gastrulation[1][2][3][4].

  • Subcellular Localization

    Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side

  • Expression


    Tissue_specificity:Protein levels were detected in the placenta (PubMed: 32337544) . High expression was observed in the lungs, liver, and kidneys.

  • Isoforms & Post-Translational Modification

    P51654 has 3 isomers: P51654-1: 65563 Da (predicted); P51654-2: 59475 Da (predicted); P51654-3: 68414 Da (predicted).
    O-glycosylated; contains heparan sulfate and/or chondroitin sulfate;Cleaved intracellularly by a furin-like convertase to generate 2 subunits, alpha and beta, which remain associated through disulfide bonds and are associated with the cell surface via the GPI-anchor (PubMed:14610063). This processing is essential for its role in inhibition of hedgehog signaling (PubMed:25653284). A second proteolytic event may result in cleavage of the protein on the cell surface, separating it from the GPI-anchor and leading to its shedding from the cell surface (PubMed:14610063)

  • Subunit

    Heterodimer; disulfide-linked (PubMed:14610063). Cleavage by a furin-like convertase results in production of alpha and beta chains which form a disulfide-linked heterodimer (PubMed:14610063). Interacts with DPP4 (PubMed:17549790). Interacts with FGF2 (By similarity). Interacts with WNT5A (PubMed:14610063). Also interacts with WNT3A and WNT7B (PubMed:16227623). Interacts with hedgehog protein SHH; the heparan sulfate chains are not required for the interaction (By similarity). Also interacts with hedgehog protein IHH (By similarity). Interacts with CD81 (By similarity). Interacts with Wnt receptors FZD4, FZD7 and FZD8; the heparan sulfate chains are required for the interaction (PubMed:24496449)

  • SwissProt ID

    P51654

  • Gene ID
    2719 [NCBI]
  • Synonyms

    DGSX; Glypican proteoglycan 3; Glypican-3 [Precursor]; Gpc3; GPC3_HUMAN; GTR2 2; GTR2-2; Heparan sulphate proteoglycan; Intestinal protein OCI 5; Intestinal protein OCI-5; MXR7; OCI 5; OCI-5; OCI5; SDYS; Secreted glypican-3; SGB; SGBS; SGBS1

References

[1]. De Cat B, et al. Processing by proprotein convertases is required for glypican-3 modulation of cell survival, Wnt signaling, and gastrulation movements. J Cell Biol. 2003 Nov 10;163(3):625-35. [Content Brief]

[2]. Capurro MI, et al. Processing by convertases is not required for glypican-3-induced stimulation of hepatocellular carcinoma growth. J Biol Chem. 2005 Dec 16;280(50):41201-6. [Content Brief]

[3]. Capurro M, et al. Glypican-3 binds to Frizzled and plays a direct role in the stimulation of canonical Wnt signaling. J Cell Sci. 2014 Apr 1;127(Pt 7):1565-75. [Content Brief]

[4]. Davoodi J, et al. The Simpson-Golabi-Behmel syndrome causative glypican-3, binds to and inhibits the dipeptidyl peptidase activity of CD26. Proteomics. 2007 Jun;7(13):2300-10. [Content Brief]

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Glypican 3 Antibody (YA5609) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Glypican 3 with Glypican 3 Antibody (HY-P85917) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Glypican 3 Antibody (HY-P85917) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Glypican 3 with Glypican 3 Antibody (HY-P85917) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Glypican 3 Antibody (HY-P85917) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Glypican 3 Antibody (YA5609) Product Image
    1/16
  • Experimental Validation Results for Glypican 3 Antibody (YA5609)
  • Experimental Validation Results for Glypican 3 Antibody (YA5609)
  • Glypican 3 Antibody (YA5609) Product Image
  • Experimental Validation Results for Glypican 3 Antibody (YA5609)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Glypican 3 with Glypican 3 Antibody (HY-P85917) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Glypican 3 Antibody (HY-P85917) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Glypican 3 Antibody (YA5609)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Glypican 3 with Glypican 3 Antibody (HY-P85917) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Glypican 3 Antibody (HY-P85917) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Glypican 3 Antibody (YA5609)
  • Experimental Validation Results for Glypican 3 Antibody (YA5609)
  • Glypican 3 Antibody (YA5609) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Glypican 3 with Glypican 3 Antibody (HY-P85917) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Glypican 3 Antibody (HY-P85917) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Glypican 3 Antibody (YA5609) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Glypican 3 with Glypican 3 Antibody (HY-P85917) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Glypican 3 Antibody (HY-P85917) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Glypican 3 Antibody (YA5609) Product Image ICC
  • Glypican 3 Antibody (YA5609) Product Image ICC