NEAS/alpha-Fodrin Antibody (YA1355)(PBS only)

Cat. No.: HY-P81610A: Data Sheet User Guide for Antibodies
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NEAS/alpha-Fodrin Antibody (YA1355) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NEAS/alpha-Fodrin.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

NEAS/alpha-Fodrin Antibody (YA1355) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to NEAS/alpha-Fodrin.

Host

Rabbit

Molecular Weight

Predicted band size: 285 kDa;

Observed band size: 285 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q13813

Gene ID

6709 [NCBI]

Immunogen

A synthetic peptide of human NEAS

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane

Subcellular Localization：Cytoplasm, cytoskeleton; Cytoplasm, cell cortex

Isoforms & Post-Translational Modification：Q13813 has 3 isomers: Q13813-1: 284539 Da (predicted); Q13813-2: 285094 Da (predicted); Q13813-3: 282282 Da (predicted).
Phosphorylation of Tyr-1176 decreases sensitivity to cleavage by calpain in vitro

Subunit：Like erythrocyte spectrin, the spectrin-like proteins are capable of forming dimers which can further associate to tetramers. Interacts (via C-terminal spectrin repeats) with TRPC4. Interacts with CALM and EMD. Interacts with isoform 1 of ACP1. Identified in a complex with ACTN4, CASK, IQGAP1, MAGI2, NPHS1 and SPTBN1. Interacts with SHANK3 (via ANK repeats). Interacts with CLN3; this interaction regulates the fodrin localization at the plasma membrane (Probable)

Research Field

Signal Transduction

Synonyms

SPTAN1; NEAS; SPTA2; Spectrin alpha chain; non-erythrocytic 1; Alpha-II spectrin; Fodrin alpha chain; Spectrin; non-erythroid alpha subunit

Documentation

User Guide for Antibodies (1077 KB)