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DUSP6 Antibody (YA2280)

(Synonyms: HH19; MKP3; PYST1; DUSP6; DUSP6a; Dual specificity phosphatase 6)
Customer Review

Based on 1 Customer Validation

DUSP6 Antibody (YA2280) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DUSP6.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Conjugation:
    Non-conjugated
ALL WB ICC IHC-P FC
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
  • DUSP6 Antibody (YA2280) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50 1:50-1:100

Product Details

Description

DUSP6 Antibody (YA2280) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to DUSP6.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 42 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 42 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human DUSP6 aa 365-381.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104640

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for DUSP6 Antibody (YA2280)
    Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), NIH/3T3 (lane 3(40μg), using DUSP6 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
    Immunocytochemistry analysis of Hela cells labeling DUSP6 with DUSP6 Antibody (HY-P82535) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DUSP6 Antibody (HY-P82535) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
    Immunocytochemistry analysis of Hela cells labeling DUSP6 with DUSP6 Antibody (HY-P82535) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DUSP6 Antibody (HY-P82535)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using DUSP6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using DUSP6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
    Flow cytometric analysis of 1.5X10^6 NIH3T3 cells labeling DUSP6 Antibody (YA2280) (HY-P82535, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Rabbit IgG H&L (AF488) (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    DUSP6 dual specificity protein phosphatase, which mediates dephosphorylation and inactivation of MAP kinases. Has a specificity for the ERK family. Plays an important role in alleviating chronic postoperative pain. Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia. Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain. Promotes cell differentiation by regulating MAPK1/MAPK3 activity and regulating the expression of AP1 transcription factors[1][2].

  • Subcellular Localization

    Cytoplasm

  • Expression


    Tissue_specificity:Expressed in keratinocytes (at protein level)

  • Isoforms & Post-Translational Modification

    Q16828 has 2 isomers: Q16828-1: 42320 Da (predicted); Q16828-2: 26468 Da (predicted).
    Ubiquitinated by the SCF(FBXO31) complex, leading to its proteasomal degradation

  • Subunit

    Interacts with MAPK1/ERK2

  • SwissProt ID

    Q16828

  • Gene ID
    1848 [NCBI]
  • Synonyms

    HH19; MKP3; PYST1; DUSP6; DUSP6a; Dual specificity phosphatase 6

  • Research Field

    Neuroscience

DUSP6 Antibody (YA2280) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), NIH/3T3 (lane 3(40μg), using DUSP6 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling DUSP6 with DUSP6 Antibody (HY-P82535) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DUSP6 Antibody (HY-P82535) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling DUSP6 with DUSP6 Antibody (HY-P82535) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DUSP6 Antibody (HY-P82535)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using DUSP6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using DUSP6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1.5X10^6 NIH3T3 cells labeling DUSP6 Antibody (YA2280) (HY-P82535, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Rabbit IgG H&L (AF488) (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • DUSP6 Antibody (YA2280) Product Image
    1/16
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
  • DUSP6 Antibody (YA2280) Product Image
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), NIH/3T3 (lane 3(40μg), using DUSP6 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling DUSP6 with DUSP6 Antibody (HY-P82535) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DUSP6 Antibody (HY-P82535) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling DUSP6 with DUSP6 Antibody (HY-P82535) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DUSP6 Antibody (HY-P82535)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using DUSP6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse brain tissue using DUSP6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1.5X10^6 NIH3T3 cells labeling DUSP6 Antibody (YA2280) (HY-P82535, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Rabbit IgG H&L (AF488) (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
  • Experimental Validation Results for DUSP6 Antibody (YA2280)
  • DUSP6 Antibody (YA2280) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from NIH/3T3 (lane 2(20μg), NIH/3T3 (lane 3(40μg), using DUSP6 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • DUSP6 Antibody (YA2280) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling DUSP6 with DUSP6 Antibody (HY-P82535) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DUSP6 Antibody (HY-P82535) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • DUSP6 Antibody (YA2280) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling DUSP6 with DUSP6 Antibody (HY-P82535) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with DUSP6 Antibody (HY-P82535)at 1/100 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • DUSP6 Antibody (YA2280) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using DUSP6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • DUSP6 Antibody (YA2280) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue using DUSP6 Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • DUSP6 Antibody (YA2280) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1.5X10^6 NIH3T3 cells labeling DUSP6 Antibody (YA2280) (HY-P82535, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Rabbit IgG H&L (AF488) (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • DUSP6 Antibody (YA2280) Product Image WB
  • DUSP6 Antibody (YA2280) Product Image ICC
  • DUSP6 Antibody (YA2280) Product Image ICC
  • DUSP6 Antibody (YA2280) Product Image IHC-P
  • DUSP6 Antibody (YA2280) Product Image IHC-P
  • DUSP6 Antibody (YA2280) Product Image FC