PRPF6 Antibody
(Synonyms: C20orf14, PRPF6, Pre-mRNA-processing factor 6, Androgen receptor N-terminal domain-transactivating protein 1, PRP6 homolog, U5 snRNP-associated 102 kDa protein, ANT-1, U5-102 kDa protein)PRPF6 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to PRPF6.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
PRPF6 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to PRPF6.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 106 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 106 kDa
Synthetic peptide corresponding to the C-term region of human PRPF6.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PRPF6 is involved in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex, one of the building blocks of the spliceosome. Enhances dihydrotestosterone-induced transactivation activity of AR, as well as dexamethasone-induced transactivation activity of NR3C1, but does not affect estrogen-induced transactivation[1][2][3].
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Subcellular Localization
Nucleus, nucleoplasm; Nucleus speckle
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Expression
Tissue_Specificity: Widely expressed. -
Isoforms & Post-Translational Modification
PRPF6 has 2 isoforms, O94906-1: amino acid length is 941, molecular weight is 106925 Da (predicted); O94906-2: amino acid length is 901, molecular weight is 102429 Da (predicted).剪接体组装过程中可被 PRP4K 磷酸化PRPF6 存在 2 个异构体，O94906-1：氨基酸个数为 941 个，分子量为 106925 Da (预测)；O94906-2：氨基酸个数为 901 个，分子量为 102429 Da (预测)。Phosphorylated by PRP4K during spliceosome assembly
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Subunit
Identified in the spliceosome B complex.
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SwissProt ID
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Synonyms
C20orf14, PRPF6, Pre-mRNA-processing factor 6, Androgen receptor N-terminal domain-transactivating protein 1, PRP6 homolog, U5 snRNP-associated 102 kDa protein, ANT-1, U5-102 kDa protein
Documentation
[1]. Schneider M, et al. Human PRP4 kinase is required for stable tri-snRNP association during spliceosomal B complex formation. Nat Struct Mol Biol. 2010 Feb;17(2):216-21. [Content Brief]
[2]. Tanackovic G, et al. A missense mutation in PRPF6 causes impairment of pre-mRNA splicing and autosomal-dominant retinitis pigmentosa. Am J Hum Genet. 2011 May 13;88(5):643-9. [Content Brief]
[3]. Bertram K, et al. Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation. Cell. 2017 Aug 10;170(4):701-713.e11. [Content Brief]
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