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eIF4A1 Antibody (YA774)

(Synonyms: Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1)
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Based on 1 publication(s) in Google Scholar

eIF4A1 Antibody (YA774) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to eIF4A1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-F, IHC-P, ICC/IF

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for eIF4A1 Antibody (YA774)
  • Experimental Validation Results for eIF4A1 Antibody (YA774)
  • Experimental Validation Results for eIF4A1 Antibody (YA774)
  • Experimental Validation Results for eIF4A1 Antibody (YA774)
  • eIF4A1 Antibody (YA774) Product Image
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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200

Product Details

Beschreibung

eIF4A1 Antibody (YA774) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to eIF4A1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 46 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 46 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to eIF4A1.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3102153

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Versand

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for eIF4A1 Antibody (YA774)
    Western blot analysis of extracts from 293T (lane 1) and Hela (lane 2) using eIF4A1 Antibody (YA774) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for eIF4A1 Antibody (YA774)
    Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), 293 (lane 2, 20 μg), HeLa (lane 3, 20 μg), MCF-7 (lane 4, 20 μg), RAW264.7 (lane 5, 20 μg), and C6 (lane 6, 20 μg) using eIF4A1 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for eIF4A1 Antibody (YA774)
    Immunocytochemistry analysis of Hela cells labeling eIF4A1 with eIF4A1 Antibody (HY-P80650) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with eIF4A1 Antibody (HY-P80650) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for eIF4A1 Antibody (YA774)
    Immunocytochemistry analysis of CACO-2 cells labeling eIF4A1 with eIF4A1 Antibody (HY-P80650) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with eIF4A1 Antibody (HY-P80650) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    eIF4A1 is an ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. As a result, promotes cell proliferation and growth[1].

  • Subcellular Localization

    Cytoplasm, perinuclear region; Cell membrane; Cytoplasm, Stress granule

  • Isoforms & Post-Translational Modification

    P60842 has 3 isomers: P60842-1: 46154 Da (predicted); P60842-2: 39548 Da (predicted); P60842-3: 22805 Da (predicted).

  • Subunit

    eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with PAIP1, EIF4E and UPF2. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. May interact with NOM1. Interacts with PDCD4; this interferes with the interaction between EIF4A and EIF4G. Interacts with RBM4. Interacts with DDX3X in an RNA-independent manner (PubMed:18596238). Interacts with PKP1 (via N-terminus); the interaction promotes EIF4A1 recruitment to the cap-dependent translation complex and EIF4A1 ATPase activity (PubMed:20156963, PubMed:23444369)

  • SwissProt ID

    P60842

  • Gene ID
    1973 [NCBI]
  • Synonyms

    Eukaryotic initiation factor 4A-I; eIF-4A-I; eIF4A-I; ATP-dependent RNA helicase eIF4A-1

  • Research Field

    Epigenetics and Nuclear Signaling

eIF4A1 Antibody (YA774) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from 293T (lane 1) and Hela (lane 2) using eIF4A1 Antibody (YA774) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), 293 (lane 2, 20 μg), HeLa (lane 3, 20 μg), MCF-7 (lane 4, 20 μg), RAW264.7 (lane 5, 20 μg), and C6 (lane 6, 20 μg) using eIF4A1 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling eIF4A1 with eIF4A1 Antibody (HY-P80650) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with eIF4A1 Antibody (HY-P80650) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of CACO-2 cells labeling eIF4A1 with eIF4A1 Antibody (HY-P80650) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with eIF4A1 Antibody (HY-P80650) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • eIF4A1 Antibody (YA774) Product Image
    1/16
  • Experimental Validation Results for eIF4A1 Antibody (YA774)
  • Experimental Validation Results for eIF4A1 Antibody (YA774)
  • Experimental Validation Results for eIF4A1 Antibody (YA774)
  • Experimental Validation Results for eIF4A1 Antibody (YA774)
  • eIF4A1 Antibody (YA774) Product Image
  • Experimental Validation Results for eIF4A1 Antibody (YA774)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from 293T (lane 1) and Hela (lane 2) using eIF4A1 Antibody (YA774) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for eIF4A1 Antibody (YA774)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), 293 (lane 2, 20 μg), HeLa (lane 3, 20 μg), MCF-7 (lane 4, 20 μg), RAW264.7 (lane 5, 20 μg), and C6 (lane 6, 20 μg) using eIF4A1 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for eIF4A1 Antibody (YA774)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling eIF4A1 with eIF4A1 Antibody (HY-P80650) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with eIF4A1 Antibody (HY-P80650) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for eIF4A1 Antibody (YA774)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of CACO-2 cells labeling eIF4A1 with eIF4A1 Antibody (HY-P80650) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with eIF4A1 Antibody (HY-P80650) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for eIF4A1 Antibody (YA774)
  • Experimental Validation Results for eIF4A1 Antibody (YA774)
  • Experimental Validation Results for eIF4A1 Antibody (YA774)
  • Experimental Validation Results for eIF4A1 Antibody (YA774)
  • eIF4A1 Antibody (YA774) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from 293T (lane 1) and Hela (lane 2) using eIF4A1 Antibody (YA774) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • eIF4A1 Antibody (YA774) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), 293 (lane 2, 20 μg), HeLa (lane 3, 20 μg), MCF-7 (lane 4, 20 μg), RAW264.7 (lane 5, 20 μg), and C6 (lane 6, 20 μg) using eIF4A1 Mouse mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • eIF4A1 Antibody (YA774) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling eIF4A1 with eIF4A1 Antibody (HY-P80650) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with eIF4A1 Antibody (HY-P80650) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • eIF4A1 Antibody (YA774) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of CACO-2 cells labeling eIF4A1 with eIF4A1 Antibody (HY-P80650) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with eIF4A1 Antibody (HY-P80650) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • eIF4A1 Antibody (YA774) Product Image WB
  • eIF4A1 Antibody (YA774) Product Image WB
  • eIF4A1 Antibody (YA774) Product Image ICC
  • eIF4A1 Antibody (YA774) Product Image ICC