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  4. Monoclonal Antibodies Recombinant Antibodies
  5. PFDN5 Antibody (YA1717)

PFDN5 Antibody (YA1717) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PFDN5.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

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  • 제품 설명

  • References

제품 설명

PFDN5 Antibody (YA1717) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to PFDN5.
Host

Rabbit
Clonality

Recombinant, Monoclonal
분자량
Predicted band size: 17 kDa;
Observed band size: 17 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human PFDN5 aa80-154.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:100
IP
IP: Immunoprecipitation
1:50
Sensitivity Endogenous Purity Affinity Chromatography
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
선적

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis was performed on extracts from 293T (lane 1, 20 μg), and HeLa (lane 2, 20 μg) using PFDN5 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (GAPDH, HY-P2804, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background
Function：Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Represses the transcriptional activity of MYC
Subcellular Localization：Nucleus; Cytoplasm; Nucleus
Expression：
Tissue_specificity:Highly expressed in pancreas and skeletal muscle and moderately in other tissues
Isoforms & Post-Translational Modification：Q99471 has 3 isomers: Q99471-1: 17328 Da (predicted); Q99471-2: 7481 Da (predicted); Q99471-3: 12379 Da (predicted).
Subunit：Heterohexamer of two PFD-alpha type and four PFD-beta type subunits. Binds to MYC; interacts with its N-terminal domain
RRID
Database

Entrez Gene: 5204 Human ; 56612 Mouse ; 300257 Rat

SwissProt: Q99471 Human ; Q9WU28 Mouse ;

OMIM: 604899 Human

Research Field

Signal Transduction
Synonyms
EIG 1; Eig1; MM1; PFD5; PFDN5; Prefoldin 5
각종 서류
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)
References

PFDN5 Antibody (YA1717) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:
PFDN5 Antibody (YA1717)
Cat. No.:
HY-P81972
수량:
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SAFETY DATA SHEET (SDS)

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