CD163 Antibody

Cat. No.: HY-P81177: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

CD163 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to CD163.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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CD163 Antibody Featured Recommendations:

2 Publications Citing Use of MCE CD163 Antibody

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

CD163 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to CD163.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 125 kDa;

Observed band size: 150 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse Predicted Reactivity: Rat,Dog,Pig,Horse

Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.

SwissProt ID

Q86VB7

Gene ID

9332 [NCBI]

Immunogen

KLH conjugated synthetic peptide derived from human CD163: 1001-1121/1156

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-2000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-500
IHC-F IHC-F: Immunohistochemistry-Frozen	1:100-500
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100-500

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.02% or 0.03% Proclin300 and 50% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from RAW264.7 using CD163 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

Function：Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells; After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions

Subcellular Localization：Secreted; Cell membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:This protein is expressed in monocytes and mature macrophages, such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident macrophages in the bone marrow, and meningeal macrophages in the central nervous system. It is also expressed in the blood. Isotype 1 is present in the lowest concentration in the blood. Isotype 2 is present in the lowest concentrations in the liver and spleen. Isotype 3 is the predominant isotype detected in the blood.

Induction:Induced by anti-inflammatory mediators such as glucocorticoids, interleukin-6/IL6 and interleukin-10/IL10; suppressed by pro-inflammatory mediators like bacterial lipopolysaccharides (LPS) , IFNG/IFN-gamma and TNF

Isoforms & Post-Translational Modification：Q86VB7 has 4 isomers: Q86VB7-1: 125451 Da (predicted); Q86VB7-2: 125982 Da (predicted); Q86VB7-3: 121609 Da (predicted); Q86VB7-4: 124958 Da (predicted).
A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs;Phosphorylated

Subunit：Interacts with CSNK2B

RRID

AB_3103089

Database

Entrez Gene: 9332 Human ; 93671 Mouse

SwissProt: Q86VB7 Human ; Q2VLH6 Mouse

OMIM: 605545 Human

Synonyms

Scavenger receptor cysteine-rich type 1 protein M130; CD 163; CD163 antigen; CD163 molecule; Hemoglobin Scavenger Receptor; M130; M130 antigen precursor; Macrophage associated antigen; MM130; C163A_HUMAN.

Documentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

CD163 Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CD163 Antibody

CD163 Antibody Featured Recommendations:

2 Publications Citing Use of MCE CD163 Antibody

Product Detail

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Background

Documentation

CD163 Antibody Related Classifications

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