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CD163 Antibody

(Synonyms: Scavenger receptor cysteine-rich type 1 protein M130; CD 163; CD163 antigen; CD163 molecule; Hemoglobin Scavenger Receptor; M130; M130 antigen precursor; Macrophage associated antigen; MM130; C163A_HUMAN.)
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Based on 2 publication(s) in Google Scholar

CD163 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to CD163.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.02% or 0.03% Proclin300 and 50% Glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for CD163 Antibody
  • CD163 Antibody Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-2000 1:100-500 1:100-500 1:100-500

Product Details

Description

CD163 Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to CD163.

  • Host Rabbit
  • Clonality Polyclonal
  • Species Reactivity
    Human, Mouse Predicted Reactivity: Rat,Dog,Pig,Horse
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 150 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 125 kDa
Species Reactivity Database
Immunogen

KLH conjugated synthetic peptide derived from human CD163: 1001-1121/1156

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3103089

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.02% or 0.03% Proclin300 and 50% Glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for CD163 Antibody
    Western blot analysis of extracts from RAW264.7 using CD163 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

  • Function

    Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells; After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions

  • Subcellular Localization

    Secreted; Cell membrane; Single-pass type I membrane protein

  • Expression


    Tissue_specificity:This protein is expressed in monocytes and mature macrophages, such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident macrophages in the bone marrow, and meningeal macrophages in the central nervous system. It is also expressed in the blood. Isotype 1 is present in the lowest concentration in the blood. Isotype 2 is present in the lowest concentrations in the liver and spleen. Isotype 3 is the predominant isotype detected in the blood.

    Induction:Induced by anti-inflammatory mediators such as glucocorticoids, interleukin-6/IL6 and interleukin-10/IL10; suppressed by pro-inflammatory mediators like bacterial lipopolysaccharides (LPS) , IFNG/IFN-gamma and TNF

  • Isoforms & Post-Translational Modification

    Q86VB7 has 4 isomers: Q86VB7-1: 125451 Da (predicted); Q86VB7-2: 125982 Da (predicted); Q86VB7-3: 121609 Da (predicted); Q86VB7-4: 124958 Da (predicted).
    A soluble form (sCD163) is produced by proteolytic shedding which can be induced by lipopolysaccharide, phorbol ester and Fc region of immunoglobulin gamma. This cleavage is dependent on protein kinase C and tyrosine kinases and can be blocked by protease inhibitors. The shedding is inhibited by the tissue inhibitor of metalloproteinase TIMP3, and thus probably induced by membrane-bound metalloproteinases ADAMs;Phosphorylated

  • Subunit

    Interacts with CSNK2B

  • SwissProt ID

    Q86VB7

  • Gene ID
    9332 [NCBI]
  • Synonyms

    Scavenger receptor cysteine-rich type 1 protein M130; CD 163; CD163 antigen; CD163 molecule; Hemoglobin Scavenger Receptor; M130; M130 antigen precursor; Macrophage associated antigen; MM130; C163A_HUMAN.

CD163 Antibody Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from RAW264.7 using CD163 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • CD163 Antibody Product Image
    1/16
  • Experimental Validation Results for CD163 Antibody
  • CD163 Antibody Product Image
  • Experimental Validation Results for CD163 Antibody
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from RAW264.7 using CD163 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for CD163 Antibody
  • CD163 Antibody Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from RAW264.7 using CD163 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • CD163 Antibody Product Image WB