MDA5 Antibody (YA7551)
(Synonyms: MDA5, RH116, IFIH1, Interferon-induced helicase C domain-containing protein 1, Clinically amyopathic dermatomyositis autoantigen 140 kDa, Helicase with 2 CARD domains, Interferon-induced with helicase C domain protein 1, Melanoma differentiation-associated protein 5, Murabutide down-regulated protein, RIG-I-like receptor 2, RNA helicase-DEAD box protein 116, CADM-140 autoantigen, Helicard, MDA-5, RLR-2)
Based on 1 Customer Validation
MDA5 Antibody (YA7551) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to MDA5.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:2000-1:10000 | 1:200-1:1000 | 1:5000-1:20000 |
Product Details
MDA5 Antibody (YA7551) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to MDA5.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 135 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 117 kDa
The exact sequence is proprietary to MCE.
Endogenous
Protein A affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
MDA5, encoded by IFIH1, functions as a cytoplasmic RNA sensor in innate immune responses[1]. Mechanistically, MDA5 recognizes long double-stranded RNA and assembles cooperative filaments that support antiviral signal activation[2][3]. ATP hydrolysis regulates filament dynamics, allowing MDA5 to discriminate viral dsRNA length during immune sensing[4]. RNA 2′-O-methylation also contributes to self-non-self RNA discrimination through an MDA5-dependent mechanism[5]. In disease models, MDA5 participates in antiviral defense against Paramyxoviridae infection in vivo[6]. Clinically, IFIH1/MDA5 is a dermatomyositis-specific autoantigen recognized by anti-CADM-140 antibody[1]. Compared with RIG-I, MDA5 preferentially detects longer dsRNA, whereas RIG-I responds more strongly to shorter dsRNA ligands[3]. This isoform distinction makes dsRNA length a practical design variable for experiments using poly (I:C) or viral RNA mimics[3].
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Subcellular Localization
Cytoplasm,Nucleus,Mitochondrion
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Expression
Tissue_Specificity: Widely expressed, at a low level. Expression is detected at slightly highest levels in placenta, pancreas and spleen and at barely levels in detectable brain, testis and lung
Induction: By interferon (IFN) and TNF -
Isoforms & Post-Translational Modification
Q9BYX4 has two isomers: Q9BYX4-1: 116689 Da (predicted); Q9BYX4-2: 25129 Da (predicted).
Sumoylated丨Ubiquitinated by RNF125, leading to its degradation by the proteasome (PubMed:17460044)丨ISGylated by ISG15丨Phosphorylated at Ser-88 (PubMed:23499489, PubMed:33727702)丨During apoptosis, processed into 3 cleavage products丨(Microbial infection) Cleaved and inactivated by the protease 2A of coxsackievirus B3, poliovirus and enterovirus 71 allowing the virus to disrupt the host type I interferon production -
Subunit
Monomer in the absence of ligands and homodimerizes in the presence of dsRNA ligands
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SwissProt ID
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Synonyms
MDA5, RH116, IFIH1, Interferon-induced helicase C domain-containing protein 1, Clinically amyopathic dermatomyositis autoantigen 140 kDa, Helicase with 2 CARD domains, Interferon-induced with helicase C domain protein 1, Melanoma differentiation-associated protein 5, Murabutide down-regulated protein, RIG-I-like receptor 2, RNA helicase-DEAD box protein 116, CADM-140 autoantigen, Helicard, MDA-5, RLR-2
Documentation
References
[1]. Nakashima R, et al. The RIG-I-like receptor IFIH1/MDA5 is a dermatomyositis-specific autoantigen identified by the anti-CADM-140 antibody. Rheumatology (Oxford). 2010 Mar;49(3):433-40. [Content Brief]
[2]. Wu B, et al. Structural basis for dsRNA recognition, filament formation, and antiviral signal activation by MDA5. Cell. 2013 Jan 17;152(1-2):276-89. [Content Brief]
[3]. Kato H, et al. Length-dependent recognition of double-stranded ribonucleic acids by retinoic acid-inducible gene-I and melanoma differentiation-associated gene 5. J Exp Med. 2008 Jul 7;205(7):1601-10. [Content Brief]
[4]. Peisley A, et al. Cooperative assembly and dynamic disassembly of MDA5 filaments for viral dsRNA recognition. Proc Natl Acad Sci U S A. 2011 Dec 27;108(52):21010-5. [Content Brief]
[5]. Züst R, et al. Ribose 2'-O-methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5. Nat Immunol. 2011 Feb;12(2):137-43. [Content Brief]
[6]. Gitlin L, et al. Melanoma differentiation-associated gene 5 (MDA5) is involved in the innate immune response to Paramyxoviridae infection in vivo. PLoS Pathog. 2010 Jan 22;6(1):e1000734. [Content Brief]
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