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Beclin 1 Antibody (YA6066)

(Synonyms: Beclin-1 (Coiled-coil myosin-like BCL2-interacting protein) (Protein GT197))
Customer Review

Based on 1 Customer Validation

Beclin 1 Antibody (YA6066) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Beclin 1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P ICC
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Beclin 1 Antibody (YA6066) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:200-1:1000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

Beclin 1 Antibody (YA6066) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Beclin 1.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 60 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 52 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719139

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
    Western blot analysis of extracts from HeLa (lane 2(20μg), HepG2 (lane 3(20μg), 3T3 (lane 4(20μg), C6 (lane 5(20μg) using Beclin 1 Antibody（HY-P86374). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
    Immunohistochemical analysis of paraffin-embedded human pancreas tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
    Immunohistochemical analysis of paraffin-embedded human testis tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
    Immunohistochemical analysis of paraffin-embedded human spleen tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
    Immunohistochemical analysis of paraffin-embedded human stomach tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
    Immunohistochemical analysis of paraffin-embedded human colon tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
    Immunocytochemistry analysis of MCF-7 cells labeling Beclin 1 with Beclin 1 antibody (HY-P86374) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Beclin 1 antibody (HY-P86374) at 1/50 dilution in BSA for Immunol Staining at 4℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
    Immunocytochemistry analysis of HeLa cells labeling Beclin 1 with Beclin 1 antibody (HY-P86374) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Beclin 1 antibody (HY-P86374) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Beclin 1, also named Atg6, is highly conserved in eukaryotes and belongs to theautophagy-related (Atg) family of proteins. Beclin 1 is a well-established regulator of autophagy, a process essential for mammalian survival. Beclin 1 performs both of its autophagy and membrane trafficking functions by interacting with several other proteins—primarily vacuolar protein sorting-associated protein 15 (VPS15), VPS34, UV radiation resistance-associated gene product (UVRAG), and autophagy-related protein 14 (ATG14) . Together, these assemble into two different Class III PI3K complexes, Complex 1 (C1) and Complex 2 (C2), depending on whether ATG14 or UVRAG respectively is present. Within these complexes, the catalytic lipid kinase subunit, VSP34, is responsible for the phosphorylation of PtdIns, which then mediates autophagy and/or membrane trafficking functions through the recruitment of effector proteins.

  • Subcellular Localization

    Cytoplasm; Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein; Endosome membrane; Peripheral membrane protein; Endoplasmic reticulum membrane; Peripheral membrane protein; Mitochondrion membrane; Peripheral membrane protein; Endosome; Cytoplasmic vesicle, autophagosome; Mitochondrion; Nucleus; Cytoplasm; Mitochondrion

  • Expression


    Tissue_specificity:general expression

  • Subunit

    A homodimeric form is proposed to exist; this metastable form readily transits to ATG14- or UVRAG-containing complexes with BECN1:UVRAG being more stable than BECN1:ATG14 (By similarity).

  • SwissProt ID

    Q14457

  • Gene ID
    8678 [NCBI]
  • Synonyms

    Beclin-1 (Coiled-coil myosin-like BCL2-interacting protein) (Protein GT197)

Beclin 1 Antibody (YA6066) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 2(20μg), HepG2 (lane 3(20μg), 3T3 (lane 4(20μg), C6 (lane 5(20μg) using Beclin 1 Antibody（HY-P86374). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Beclin 1 with Beclin 1 antibody (HY-P86374) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Beclin 1 antibody (HY-P86374) at 1/50 dilution in BSA for Immunol Staining at 4℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling Beclin 1 with Beclin 1 antibody (HY-P86374) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Beclin 1 antibody (HY-P86374) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Beclin 1 Antibody (YA6066) Product Image
    1/16
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Beclin 1 Antibody (YA6066) Product Image
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 2(20μg), HepG2 (lane 3(20μg), 3T3 (lane 4(20μg), C6 (lane 5(20μg) using Beclin 1 Antibody（HY-P86374). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human pancreas tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human stomach tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human colon tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Beclin 1 with Beclin 1 antibody (HY-P86374) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Beclin 1 antibody (HY-P86374) at 1/50 dilution in BSA for Immunol Staining at 4℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling Beclin 1 with Beclin 1 antibody (HY-P86374) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Beclin 1 antibody (HY-P86374) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Experimental Validation Results for Beclin 1 Antibody (YA6066)
  • Beclin 1 Antibody (YA6066) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 2(20μg), HepG2 (lane 3(20μg), 3T3 (lane 4(20μg), C6 (lane 5(20μg) using Beclin 1 Antibody（HY-P86374). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/3000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001 ,1/10,000) was used for 1 hour at room temperature.

  • Beclin 1 Antibody (YA6066) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Beclin 1 Antibody (YA6066) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human testis tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Beclin 1 Antibody (YA6066) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Beclin 1 Antibody (YA6066) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Beclin 1 Antibody (YA6066) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human stomach tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Beclin 1 Antibody (YA6066) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human colon tissue using Beclin 1 Antibody (HY-P86374, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Beclin 1 Antibody (YA6066) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling Beclin 1 with Beclin 1 antibody (HY-P86374) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Beclin 1 antibody (HY-P86374) at 1/50 dilution in BSA for Immunol Staining at 4℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Beclin 1 Antibody (YA6066) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling Beclin 1 with Beclin 1 antibody (HY-P86374) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Beclin 1 antibody (HY-P86374) at 1/50 dilution in BSA for Immunol Staining at 4 ℃,Stay overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Beclin 1 Antibody (YA6066) Product Image WB
  • Beclin 1 Antibody (YA6066) Product Image IHC-P
  • Beclin 1 Antibody (YA6066) Product Image IHC-P
  • Beclin 1 Antibody (YA6066) Product Image IHC-P
  • Beclin 1 Antibody (YA6066) Product Image IHC-P
  • Beclin 1 Antibody (YA6066) Product Image IHC-P
  • Beclin 1 Antibody (YA6066) Product Image IHC-P
  • Beclin 1 Antibody (YA6066) Product Image ICC
  • Beclin 1 Antibody (YA6066) Product Image ICC