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TCF3 / E2A Antibody (YA3815)

(Synonyms: E2A; ITF1; VDIR; bHLHb21; MGC129647; MGC129648; TCF3)
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Based on 1 Customer Validation

TCF3 / E2A Antibody (YA3815) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TCF3 / E2A.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide.

  • Conjugation:
    Non-conjugated
ALL WB FC
  • Experimental Validation Results for TCF3 / E2A Antibody (YA3815)
  • Experimental Validation Results for TCF3 / E2A Antibody (YA3815)
  • Experimental Validation Results for TCF3 / E2A Antibody (YA3815)
  • TCF3 / E2A Antibody (YA3815) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:500-1:2000 1:200-1:400 1:10000

Product Details

Description

TCF3 / E2A Antibody (YA3815) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TCF3 / E2A.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 68 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 67.6 kDa
Immunogen

Purified recombinant fragment of human TCF3 aa 1-200.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718840

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for TCF3 / E2A Antibody (YA3815)
    Western blot analysis of extracts from Ramos (lane 2(20μg), K562 (lane 3(20μg), HepG2 (lane 4(20μg), MCF-7 (lane 5(20μg) using TCF3 / E2A Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TCF3 / E2A Antibody (YA3815)
    Western blot analysis of extracts from HCT116(lane 1(40μg) ),PC-3(lane 2(40μg) ),COS-7(lane 3(40μg) ),Jurkat(lane 4(40μg) ),Raji(lane 5(40μg) ),Hela(lane 6(40μg) )and A549(lane 7(40μg) ) using TCF3 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH， 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TCF3 / E2A Antibody (YA3815)
    Flow cytometric analysis of 1X10^6 Ramos cells labeling TCF3 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    TCF3 / E2A transcriptional regulator involved in the initiation of neuronal differentiation and mesenchymal to epithelial transition. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Together with TCF15, required for the mesenchymal to epithelial transition. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region; Facilitates ATOH7 binding to DNA at the consensus sequence 5'-CAGGTG-3', and positively regulates transcriptional activity[1].

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    P15923 has 3 isomers: P15923-1: 67600 Da (predicted); P15923-2: 67265 Da (predicted); P15923-3: 68510 Da (predicted).
    Phosphorylated following NGF stimulation;Undergoes Notch-induced ubiquitination and subsequent proteasomal degradation which is mediated by ASB1 or ASB2, the substrate-recognition components of probable ECS E3 ubiquitin-protein ligase complexes

  • Subunit

    Homodimer (PubMed:14752053, PubMed:2112746). Heterodimer; efficient DNA binding requires dimerization with another bHLH protein (By similarity). Forms a heterodimer with ASH1, TWIST1 and TWIST2 (By similarity). Forms a heterodimer with MYOG; heterodimerization enhances MYOG DNA-binding and transcriptional activities (By similarity). Forms a heterodimer with NEUROD1; the heterodimer is inhibited in presence of ID2, but not NR0B2, to E-box element (PubMed:14752053). Forms a heterodimer with TCF15; the heterodimer binds E-box element (By similarity). Forms a heterodimer with ATOH8; repress transcription of TCF3 and TCF3/NEUROG3 dimer-induced transactivation of E box-dependent promoters (By similarity). Component of a nuclear TAL-1 complex composed at least of CBFA2T3, LDB1, TAL1 and TCF3 (By similarity). Interacts with NEUROD2, PTF1A and TGFB1I1 (By similarity). Interacts with EP300 and UBE2I (PubMed:14752053, PubMed:9409784). Interacts with BHLHA9 (PubMed:25466284). Interacts with ASB2; the interaction is mediated by SKP2 and targets TCF3 for Notch-induced proteasomal degradation (PubMed:21119685)

  • SwissProt ID

    P15923

  • Gene ID
    6929 [NCBI]
  • Synonyms

    E2A; ITF1; VDIR; bHLHb21; MGC129647; MGC129648; TCF3

TCF3 / E2A Antibody (YA3815) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Ramos (lane 2(20μg), K562 (lane 3(20μg), HepG2 (lane 4(20μg), MCF-7 (lane 5(20μg) using TCF3 / E2A Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HCT116(lane 1(40μg) ),PC-3(lane 2(40μg) ),COS-7(lane 3(40μg) ),Jurkat(lane 4(40μg) ),Raji(lane 5(40μg) ),Hela(lane 6(40μg) )and A549(lane 7(40μg) ) using TCF3 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH， 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Ramos cells labeling TCF3 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • TCF3 / E2A Antibody (YA3815) Product Image
    1/16
  • Experimental Validation Results for TCF3 / E2A Antibody (YA3815)
  • Experimental Validation Results for TCF3 / E2A Antibody (YA3815)
  • Experimental Validation Results for TCF3 / E2A Antibody (YA3815)
  • TCF3 / E2A Antibody (YA3815) Product Image
  • Experimental Validation Results for TCF3 / E2A Antibody (YA3815)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Ramos (lane 2(20μg), K562 (lane 3(20μg), HepG2 (lane 4(20μg), MCF-7 (lane 5(20μg) using TCF3 / E2A Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TCF3 / E2A Antibody (YA3815)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HCT116(lane 1(40μg) ),PC-3(lane 2(40μg) ),COS-7(lane 3(40μg) ),Jurkat(lane 4(40μg) ),Raji(lane 5(40μg) ),Hela(lane 6(40μg) )and A549(lane 7(40μg) ) using TCF3 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH， 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for TCF3 / E2A Antibody (YA3815)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Ramos cells labeling TCF3 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for TCF3 / E2A Antibody (YA3815)
  • Experimental Validation Results for TCF3 / E2A Antibody (YA3815)
  • Experimental Validation Results for TCF3 / E2A Antibody (YA3815)
  • TCF3 / E2A Antibody (YA3815) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Ramos (lane 2(20μg), K562 (lane 3(20μg), HepG2 (lane 4(20μg), MCF-7 (lane 5(20μg) using TCF3 / E2A Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • TCF3 / E2A Antibody (YA3815) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HCT116(lane 1(40μg) ),PC-3(lane 2(40μg) ),COS-7(lane 3(40μg) ),Jurkat(lane 4(40μg) ),Raji(lane 5(40μg) ),Hela(lane 6(40μg) )and A549(lane 7(40μg) ) using TCF3 antibody. Proteins were transferred to a NC membrane and blocked with 5% Skim milk in TBST for 2 hour at room temperature. The primary antibody ( 1/1000) and Loading control antibody (GAPDH， 1/1000) was used in 5% Skim milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • TCF3 / E2A Antibody (YA3815) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Ramos cells labeling TCF3 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • TCF3 / E2A Antibody (YA3815) Product Image WB
  • TCF3 / E2A Antibody (YA3815) Product Image WB
  • TCF3 / E2A Antibody (YA3815) Product Image FC