Transcription Initiation Factor IIB Antibody (YA1055)
(Synonyms: General transcription factor IIB; General transcription factor TFIIB; gtf2b; RNA polymerase II transcription factor IIB; S300 II; S300-II; TF IIB; TF2B; TF2B_HUMAN; TFIIB; Transcription initiation factor IIB.)
Based on 1 Customer Validation
Transcription Initiation Factor IIB Antibody (YA1055) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transcription Initiation Factor IIB.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IP
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IP
IP: Immunoprecipitation
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|---|---|---|
| Dilution Ratio | 1:500-1:1000 | 1:50 |
Product Details
Transcription Initiation Factor IIB Antibody (YA1055) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transcription Initiation Factor IIB.
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Host Rabbit
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Clonality Recombinant,Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 31 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 35 kDa
Entrez Gene: 2959 Human ; 229906 Mouse ; 81673 Rat
SwissProt: Q00403 Human ; P62915 Mouse ; P62916 Rat
OMIM: 189963 Human
A synthesized peptide derived from human TF2B aa250-316/316.
Endogenous
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from RAW264.7 (lane 2(20μg), RAW264.7 (lane 3(40μg), using Transcription Initiation Factor IIB Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
Background
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Function
Transcription Initiation Factor IIB is a General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA. Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex. Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle. Associates with chromatin to core promoter-specific regions. Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element. Modulates transcription start site selection. Also exhibits autoacetyltransferase activity that contributes to the activated transcription[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19].
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Subcellular Localization
Nucleus; Chromosome
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Expression
Tissue_specificity:It is expressed in the inner cell mass that forms the blastocyst (PubMed:24441171) . It was not detected (at the protein level) in the outer thin trophoblast cells (PubMed:24441171) . -
Subunit
Found in a ternary complex with TATA box-bound TBP (PubMed:10619841, PubMed:29158257, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312).
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SwissProt ID
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Synonyms
General transcription factor IIB; General transcription factor TFIIB; gtf2b; RNA polymerase II transcription factor IIB; S300 II; S300-II; TF IIB; TF2B; TF2B_HUMAN; TFIIB; Transcription initiation factor IIB.
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Research Field
Epigenetics and Nuclear Signaling
Documentation
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Data Sheet (262 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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User Guide for Antibodies (1077 KB)
References
[1]. Choi CH, et al. Transcription factor IIB acetylates itself to regulate transcription. Nature. 2003 Aug 21;424(6951):965-9. [Content Brief]
[2]. Ing NH, et al. Members of the steroid hormone receptor superfamily interact with TFIIB (S300-II). J Biol Chem. 1992 Sep 5;267(25):17617-23. [Content Brief]
[3]. Ha I, et al. Cloning of a human gene encoding the general transcription initiation factor IIB. Nature. 1991 Aug 22;352(6337):689-95. [Content Brief]
[4]. Malik S, et al. Sequence of general transcription factor TFIIB and relationships to other initiation factors. Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9553-7. [Content Brief]
[5]. He Y, et al. Near-atomic resolution visualization of human transcription promoter opening. Nature. 2016 May 19;533(7603):359-65. [Content Brief]
[6]. Reinberg D, et al. Factors involved in specific transcription by mammalian RNA polymerase II. Purification and functional analysis of initiation factors IIB and IIE. J Biol Chem. 1987 Mar 5;262(7):3310-21. [Content Brief]
[7]. Reinberg D, et al. Factors involved in specific transcription in mammalian RNA polymerase II. Functional analysis of initiation factors IIA and IID and identification of a new factor operating at sequences downstream of the initiation site. J Biol Chem. 1987 Mar 5;262(7):3322-30. [Content Brief]
[8]. Zawel L, et al. Recycling of the general transcription factors during RNA polymerase II transcription. Genes Dev. 1995 Jun 15;9(12):1479-90. [Content Brief]
[9]. Malik S, et al. Potential RNA polymerase II-induced interactions of transcription factor TFIIB. Mol Cell Biol. 1993 Oct;13(10):6253-9. [Content Brief]
[10]. Hisatake K, et al. Functional dissection of TFIIB domains required for TFIIB-TFIID-promoter complex formation and basal transcription activity. Nature. 1993 Jun 24;363(6431):744-7. [Content Brief]
[11]. Barberis A, et al. Delineation of two functional regions of transcription factor TFIIB. Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5628-32. [Content Brief]
[12]. Buratowski S, et al. Functional domains of transcription factor TFIIB. Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5633-7. [Content Brief]
[13]. Lagrange T, et al. New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor IIB. Genes Dev. 1998 Jan 1;12(1):34-44. [Content Brief]
[14]. Ha I, et al. Multiple functional domains of human transcription factor IIB: distinct interactions with two general transcription factors and RNA polymerase II. Genes Dev. 1993 Jun;7(6):1021-32. [Content Brief]
[15]. Gelev V, et al. A new paradigm for transcription factor TFIIB functionality. Sci Rep. 2014 Jan 20;4:3664. [Content Brief]
[16]. Tsai FT, et al. Structural basis of preinitiation complex assembly on human pol II promoters. EMBO J. 2000 Jan 4;19(1):25-36. [Content Brief]
[17]. Deng W, et al. A core promoter element downstream of the TATA box that is recognized by TFIIB. Genes Dev. 2005 Oct 15;19(20):2418-23. [Content Brief]
[18]. Nikolov DB, et al. Crystal structure of a TFIIB-TBP-TATA-element ternary complex. Nature. 1995 Sep 14;377(6545):119-28. [Content Brief]
[19]. Hawkes NA, et al. The role of human TFIIB in transcription start site selection in vitro and in vivo. J Biol Chem. 1999 May 14;274(20):14337-43. [Content Brief]
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