Transcription Initiation Factor IIB Antibody (YA1055)

Cat. No.: HY-P81312A: Data Sheet COA
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Transcription Initiation Factor IIB Antibody (YA1055) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transcription Initiation Factor IIB.

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10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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Transcription Initiation Factor IIB Antibody (YA1055) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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제품 설명

Transcription Initiation Factor IIB Antibody (YA1055) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Transcription Initiation Factor IIB.

Host

Rabbit

Clonality

Recombinant,Monoclonal

분자량

Predicted band size: 35 kDa;

Observed band size: 31 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q00403

Gene ID

2959 [NCBI]

Immunogen

A synthesized peptide derived from human TF2B aa250-316/316.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IP IP: Immunoprecipitation	1:50

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from RAW264.7 (lane 2(20μg), RAW264.7 (lane 3(40μg), using Transcription Initiation Factor IIB Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

Background

Function：General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:12931194, PubMed:1517211, PubMed:1876184, PubMed:1946368, PubMed:27193682, PubMed:3029109, PubMed:3818643, PubMed:7601352, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:9420329). Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8413225, PubMed:8504927, PubMed:8515820, PubMed:8516311, PubMed:8516312). Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352). Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171). Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:10619841, PubMed:16230532, PubMed:7675079, PubMed:9420329). Modulates transcription start site selection (PubMed:10318856). Also exhibits autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194)

Subcellular Localization：Nucleus; Chromosome

Expression：
Tissue_specificity:It is expressed in the inner cell mass that forms the blastocyst (PubMed:24441171) . It was not detected (at the protein level) in the outer thin trophoblast cells (PubMed:24441171) .

Subunit：Found in a ternary complex with TATA box-bound TBP (PubMed:10619841, PubMed:29158257, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312).

RRID

AB_3103426

Database

Entrez Gene: 2959 Human ; 229906 Mouse ; 81673 Rat

SwissProt: Q00403 Human ; P62915 Mouse ; P62916 Rat

OMIM: 189963 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

General transcription factor IIB; General transcription factor TFIIB; gtf2b; RNA polymerase II transcription factor IIB; S300 II; S300-II; TF IIB; TF2B; TF2B_HUMAN; TFIIB; Transcription initiation factor IIB.

각종 서류

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Data Sheet (232 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Transcription Initiation Factor IIB Antibody (YA1055) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Transcription Initiation Factor IIB Antibody (YA1055)

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Transcription Initiation Factor IIB Antibody (YA1055) Related Classifications

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