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Phospho-RIP(S166) Antibody (YA1284)

(Synonyms: Cell death protein RIP,Receptor-interacting protein 1)
2 Cited Publications
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Based on 2 publication(s) in Google Scholar

Phospho-RIP(S166) Antibody (YA1284) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-RIP(S166).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, IHC-F, ICC/IF, IP

  • Reactivity :

    Mouse

  • Formulation:

    Supplied in PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.

  • Conjugation:
    Non-conjugated
  • Phospho-RIP(S166) Antibody (YA1284) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
IP Info
IP: Immunoprecipitation
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
Dilution Ratio 1:1000-1:2000 1:50-1:500 1:100-1:500 1:100-1:500 1:100-1:500

Product Details

Description

Phospho-RIP(S166) Antibody (YA1284) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Phospho-RIP(S166).

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Mouse
  • Observed Molecular Weight
    Observed band size:75 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 75 kDa,
Species Reactivity Database
Immunogen

A synthetic phospho-peptide corresponding to residues surrounding serine 166 of Mouse RIPK1

Sensitivity

Endogenous

Purification

ProA affinity purified IgG

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3644174

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    RIP is a Serine-threonine kinase which is a key regulator of TNF-mediated apoptosis, necroptosis and inflammatory pathways. Exhibits kinase activity-dependent functions that regulate cell death and kinase-independent scaffold functions regulating inflammatory signaling and cell survival. Has kinase-independent scaffold functions: upon binding of TNF to TNFR1, RIPK1 is recruited to the TNF-R1 signaling complex (TNF-RSC also known as complex I) where it acts as a scaffold protein promoting cell survival, in part, by activating the canonical NF-kappa-B pathway. Kinase activity is essential to regulate necroptosis and apoptosis, two parallel forms of cell death: upon activation of its protein kinase activity, regulates assembly of two death-inducing complexes, namely complex IIa (RIPK1-FADD-CASP8), which drives apoptosis, and the complex IIb (RIPK1-RIPK3-MLKL), which drives necroptosis. RIPK1 is required to limit CASP8-dependent TNFR1-induced apoptosis. In normal conditions, RIPK1 acts as an inhibitor of RIPK3-dependent necroptosis, a process mediated by RIPK3 component of complex IIb, which catalyzes phosphorylation of MLKL upon induction by ZBP1. Inhibits RIPK3-mediated necroptosis via FADD-mediated recruitment of CASP8, which cleaves RIPK1 and limits TNF-induced necroptosis. Required to inhibit apoptosis and necroptosis during embryonic development: acts by preventing the interaction of TRADD with FADD thereby limiting aberrant activation of CASP8. In addition to apoptosis and necroptosis, also involved in inflammatory response by promoting transcriptional production of pro-inflammatory cytokines, such as interleukin-6 (IL6). Phosphorylates RIPK3: RIPK1 and RIPK3 undergo reciprocal auto- and trans-phosphorylation. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Required for ZBP1-induced NF-kappa-B activation in response to DNA damage[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19].

  • Subcellular Localization

    Cytoplasm; Cell membrane

  • Expression


    Tissue_specificity:Found at low levels in all tissues

    Induction:In concanavalin A-treated splenocytes

  • Subunit

    Homodimer (PubMed:29440439). Interacts (via RIP homotypic interaction motif) with RIPK3 (via RIP homotypic interaction motif); this interaction induces RIPK1 phosphorylation and formation of a RIPK1-RIPK3 necroptosis-inducing complex (PubMed:28842570, PubMed:31519887). Upon TNF-induced necrosis, the RIPK1-RIPK3 dimer further interacts with PGAM5 and MLKL; the formation of this complex leads to PGAM5 phosphorylation and increase in PGAM5 phosphatase activity (By similarity). Interacts (via the death domain) with TNFRSF6 (via the death domain) and TRADD (via the death domain) (By similarity). Is recruited by TRADD to TNFRSF1A in a TNF-dependent process (By similarity). Binds RNF216, EGFR, IKBKG, TRAF1, TRAF2 and TRAF3 (By similarity). Interacts with BNLF1 (By similarity). Interacts with SQSTM1 upon TNF-alpha stimulation (By similarity). May interact with MAVS/IPS1 (By similarity). Interacts with ZFAND5 (By similarity). Interacts with RBCK1 (By similarity). Interacts with ZBP1 (PubMed:19590578, PubMed:23283962, PubMed:33397971). Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2 and XIAP/BIRC4. Interacts (via kinase domain) with DAB2IP (via Ras-GAP domain); the interaction occurs in a TNF-alpha-dependent manner (By similarity). Interacts with ARHGEF2 (By similarity). Interacts (via protein kinase domain) with RFFL; involved in RIPK1 ubiquitination (By similarity). Interacts with RNF34; involved in RIPK1 ubiquitination (By similarity). Interacts with TICAM1 and this interaction is enhanced in the presence of WDFY1 (By similarity). Interacts with PELI1 (PubMed:29883609). Interacts (via death domain) with CRADD (via death domain); the interaction is direct (By similarity). Component of complex IIa composed of at least RIPK1, FADD and CASP8 (PubMed:29440439). Component of the AIM2 PANoptosome complex, a multiprotein complex that drives inflammatory cell death (PANoptosis) (PubMed:34471287). Interacts with MAP3K7, CFLAR and CASP8 (PubMed:31519887). Interacts with FADD (PubMed:28842570, PubMed:29440439, PubMed:31519887). Interacts with NEMO (PubMed:31519886). Interacts with TAX1BP1; this interaction negatively regulates RIPK1 ubiquitination (PubMed:18239685). Interacts with GRB2 (By similarity). Interacts with DDX24; this interaction disrupts RLR signaling activation of IFN-dependent transcription factor IRF7 (By similarity)

  • SwissProt ID

    Q60855

  • Gene ID
    8737 [NCBI]
  • Synonyms

    Cell death protein RIP,Receptor-interacting protein 1

  • Research Field

    Cell Biology

Phospho-RIP(S166) Antibody (YA1284) Related Classifications

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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