2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. Ubiquitin D Antibody (YA021)

Ubiquitin D Antibody (YA021)

Cat. No.: HY-P80364
COA
SDS
User Guide for Antibodies Technical Support

Ubiquitin D Antibody (YA021) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ubiquitin D.

For research use only. We do not sell to patients.

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Top Publications Citing Use of Products

1 Publications Citing Use of MCE Ubiquitin D Antibody (YA021)

  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

  • Background

  • Documentation

  • References

Description

Ubiquitin D Antibody (YA021) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Ubiquitin D.
Host

Rabbit
Clonality

Recombinant, Monoclonal
Molecular Weight
Predicted band size: 18 kDa;
Observed band size: 23 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

Synthetic peptide corresponding to Human Ubiquitin D.AA range:114-158.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000-1:2000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:100-1:500
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:200
Sensitivity Endogenous Purity Protein A affinity purified.
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from Ramos using Ubiquitin D antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background
Function：Ubiquitin-like protein modifier which can be covalently attached to target proteins and subsequently leads to their degradation by the 26S proteasome, in a NUB1-dependent manner (PubMed:15831455, PubMed:16707496, PubMed:19166848). Conjugation to the target protein is activated by UBA6 via adenylation of its C-terminal glycine (PubMed:17889673, PubMed:35970836). Promotes the expression of the proteasome subunit beta type-9 (PSMB9/LMP2). Regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha (PubMed:19959714). Required for TNF-alpha-induced p65 nuclear translocation in renal tubular epithelial cells (RTECs). May be involved in dendritic cell (DC) maturation, the process by which immature dendritic cells differentiate into fully competent antigen-presenting cells that initiate T-cell responses (PubMed:19028597). Mediates mitotic non-disjunction and chromosome instability, in long-term in vitro culture and cancers, by abbreviating mitotic phase and impairing the kinetochore localization of MAD2L1 during the prometaphase stage of the cell cycle (PubMed:16495226). May be involved in the formation of aggresomes when proteasome is saturated or impaired (PubMed:19033385). Mediates apoptosis in a caspase-dependent manner, especially in renal epithelium and tubular cells during renal diseases such as polycystic kidney disease and Human immunodeficiency virus (HIV)-associated nephropathy (HIVAN) (PubMed:16495380)
Subcellular Localization：Nucleus; Cytoplasm
Expression：
Tissue_specificity:Constitutively expressed in mature dendritic cells and B-cells. Mostly expressed in the reticuloendothelial system (e.g. thymus, spleen) , the gastrointestinal system, kidney, lung and prostate gland

Induction:Rapidly degraded by the proteasome. Cell-cycle regulation with highest expression during the S-phase (at protein level) . Induced during dendritic cell maturation. Negatively regulated by p53/TP53. High levels in various gastrointestinal and gynecological cancer cells. Induced in RTECs in common renal diseases including diabetic nephropathy (DN) , IgA nephropathy (IgAN) , and hypertensive nephrosclerosis (HN) , as well as in hepatocellular carcinoma (HCC) and during HIVAN. Inducible by the pro-inflammatory cytokines IFNG/IFN-gamma and TNF in cancers of liver and colon. Repressed by NUB1 (at protein level)
Subunit：Interacts directly with the 26S proteasome (PubMed:16707496). Interacts with NUB1; this interaction facilitates the linking of UBD-conjugated target protein to the proteasome complex and accelerates its own degradation and that of its conjugates (PubMed:14757770, PubMed:16707496, PubMed:25422469). Interacts (via ubiquitin-like 1 domain) with the spindle checkpoint protein MAD2L1 during mitosis (PubMed:10200259, PubMed:16495226, PubMed:25422469). Present in aggresomes of proteasome inhibited cells. Interacts with HDAC6 under proteasome impairment conditions (PubMed:19033385, PubMed:25422469). Forms a thioester with UBA6 in cells stimulated with tumor necrosis factor-alpha (TNFa) and interferon-gamma (IFNg) (PubMed:17889673, PubMed:25422469). Interacts with SQSTM1 and TP53/p53 (PubMed:25422469)
RRID
Database

Entrez Gene: 10537 Human ; 24108 Mouse ; 29168 Rat

SwissProt: O15205 Human ; P63072 Mouse ; Q921A3 Rat

OMIM: 606050 Human

Research Field

Microbiology
Documentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)
References

Ubiquitin D Antibody (YA021) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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