S100A9 Antibody(YA3464)
(Synonyms: Leukocyte L1 complex heavy chain antibody; 60B8AG antibody; CAGB antibody; Calgranulin B antibody; Calgranulin-B antibody; Calprotectin L1H subunit antibody; CFAG antibody; CGLB antibody; Cystic fibrosis antigen B antibody; L1AG antibody; Leukocyte L1 complex heavy chain antibody; LIAG antibody; MAC387 antibody; MIF antibody; Migration inhibitory factor related protein 14 antibody; Migration inhibitory factor-related protein 14 antibody; MRP 14 antibody; MRP-14 antibody; MRP14 antibody; Myeloid-related protein 14 antibody; NIF antibody; OTTHUMP00000015331 antibody; p14 antibody; Protein S100-A9 antibody; S100 A9 antibody; S100 calcium binding protein A9 antibody; S100 calcium binding protein A9 calgranulin B antibody; S100 calcium-binding protein A9 antibody; S100A9 antibody; S10A9_HUMAN antibody; )Based on 1 Customer Validation
S100A9 Antibody(YA3464) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to S100A9.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, IF-Tissue, mIHC
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Reactivity :
Human
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Formulation:
Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
Applications
| Application |
WB
WB: Western Blot
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IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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mIHC
mIHC: Multiplex Immunohistochemical
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| Dilution Ratio | 1:1000 | 1:50-1:200 | 1:1000-1:8000 | 1:1000 |
Product Details
S100A9 Antibody(YA3464) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to S100A9.
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Host Rabbit
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Clonality Monoclonal,Recombinant
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 13 kDaNote: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 13 kDa
Synthetic peptide within Human S100A9 aa 1-42 / 114.
Affinity Purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using S100A9 Antibody (HY-P83734, 1/5000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using S100A9 Antibody (HY-P83734, 1/5000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using S100A9 Antibody (HY-P83734, 1/5000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using S100A9 Antibody (HY-P83734, 1/5000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human skin cancer tissue using S100A9 Antibody (HY-P83734, 1/5000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using S100A9 Antibody (HY-P83734, 1/5000). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Background
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Function
S100A9 is a calcium-binding S100 family protein released mainly by neutrophils and monocytes, and it commonly functions with S100A8 as the S100A8/A9 calprotectin heterodimer in innate immune inflammation[1]. Mechanistically, extracellular S100A9 engages TLR4 with CD14 as a co-receptor, inducing NF-κB activation and a pro-inflammatory cytokine response in monocytes[2]. The S100A8/A9 complex also activates inflammatory signaling through TLR4, RAGE, NF-κB, and MAPK pathways, linking leukocyte recruitment, cytokine secretion, infection defense, and inflammation-associated disease models[1][3]. In tumor models, S100A9-TLR4 signaling promoted tumor growth, whereas S100A9 deficiency, TLR4 deficiency, or ABR-215050/tasquinimod reduced EL4 lymphoma growth[4]. Compared with related isoforms, S100A9 differs from S100A8 and S100A12 because its best-characterized inflammatory role often depends on heterodimerization with S100A8 and receptor-specific TLR4/CD14 signaling[1][2][3]. For experimental applications, paquinimod reduced synovial activation, osteophyte formation, and cartilage damage in collagenase-induced osteoarthritis with high synovial activation[5].- S100A9 supports inflammation studies through TLR4/CD14-dependent cytokine induction in monocyte models[2]. - S100A9 inhibitors enable disease-model testing in tumor growth and osteoarthritis pathology[4][5].
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Subcellular Localization
Secreted; Cytoplasm; Cytoplasm, cytoskeleton; Cell membrane; Peripheral membrane protein
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Expression
Tissue_specificity:Calprotectin (S100A8/9) is primarily expressed in myeloid cells. Except in inflammatory states, its expression is limited to specific stages of myeloid differentiation, as both proteins are expressed in circulating neutrophils and monocytes, but absent in normal tissue macrophages and lymphocytes. Calprotectin is also expressed in the epidermis in cH2O2ic inflammatory states, such as psoriasis and malignancies. Calprotectin concentrations are higher in serum at sites of inflammation or in patients with inflammatory diseases such as rheumatoid arthritis, cystic fibrosis, inflammatory bowel disease, Crohn's disease, giant cell arteritis, Sjgren's syndrome, systemic lupus erythematosus, and progressive systemic sclerosis. Calprotectin is involved in the formation and deposition of amyloid proteins (called amyloid inclusion bodies) in the aging prostate. It is significantly upregulated in a variety of tumors, including gastric cancer, esophageal cancer, colon cancer, pancreatic cancer, bladder cancer, ovarian cancer, thyroid cancer, breast cancer, and skin cancer.
Positive sample: Human cervical cancer, human breast cancer tissue, human spleen tissue. -
Subunit
Homodimer (PubMed:11851337, PubMed:16258195). Preferentially exists as a heterodimer or heterotetramer with S100A8 known as calprotectin (S100A8/A9) (PubMed:16258195, PubMed:17553524, PubMed:19087201, PubMed:19122197, PubMed:25417112, PubMed:8423249, PubMed:9083090).
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SwissProt ID
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Synonyms
Leukocyte L1 complex heavy chain antibody; 60B8AG antibody; CAGB antibody; Calgranulin B antibody; Calgranulin-B antibody; Calprotectin L1H subunit antibody; CFAG antibody; CGLB antibody; Cystic fibrosis antigen B antibody; L1AG antibody; Leukocyte L1 complex heavy chain antibody; LIAG antibody; MAC387 antibody; MIF antibody; Migration inhibitory factor related protein 14 antibody; Migration inhibitory factor-related protein 14 antibody; MRP 14 antibody; MRP-14 antibody; MRP14 antibody; Myeloid-related protein 14 antibody; NIF antibody; OTTHUMP00000015331 antibody; p14 antibody; Protein S100-A9 antibody; S100 A9 antibody; S100 calcium binding protein A9 antibody; S100 calcium binding protein A9 calgranulin B antibody; S100 calcium-binding protein A9 antibody; S100A9 antibody; S10A9_HUMAN antibody;
Documentation
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Data Sheet (262 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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User Guide for Antibodies (1077 KB)
References
[1]. Wang S, et al. S100A8/A9 in Inflammation. Front Immunol. 2018 Jun 11;9:1298. [Content Brief]
[2]. He Z, et al. CD14 Is a Co-Receptor for TLR4 in the S100A9-Induced Pro-Inflammatory Response in Monocytes. PLoS One. 2016 May 26;11(5):e0156377. [Content Brief]
[3]. Xia P, et al. Roles of S100A8, S100A9 and S100A12 in infection, inflammation and immunity. Immunology. 2024 Mar;171(3):365-376. [Content Brief]
[4]. Källberg E, et al. S100A9 interaction with TLR4 promotes tumor growth. PLoS One. 2012;7(3):e34207. [Content Brief]
[5]. Schelbergen RF, et al. Prophylactic treatment with S100A9 inhibitor paquinimod reduces pathology in experimental collagenase-induced osteoarthritis. Ann Rheum Dis. 2015 Dec;74(12):2254-8. [Content Brief]