SAP49 Antibody
(Synonyms: SAP49, SF3B4, Splicing factor 3B subunit 4, Pre-mRNA-splicing factor SF3b 49 kDa subunit, Spliceosome-associated protein 49, SAP 49)SAP49 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to SAP49.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 |
Product Details
SAP49 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to SAP49.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 49 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 44 kDa
Synthetic peptide corresponding to the center region of human SAP49.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
SAP49 is a Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3B4 is part of the SF3B subcomplex, which is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA. Sequence independent binding of SF3A and SF3B subcomplexes upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex. Also acts as a component of the minor spliceosome, which is involved in the splicing of U12-type introns in pre-mRNAs[1][2][3][4][5][6].
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Subcellular Localization
Nucleus
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Isoforms & Post-Translational Modification
SAP49 has an amino acid length of 424, molecular weight is 44386 Da.SAP49 的氨基酸个数为 424 个，分子量为 44386 Da。
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Subunit
Component of the 17S U2 SnRNP complex, a ribonucleoprotein complex that contains small nuclear RNA (snRNA) U2 and a number of specific proteins.
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SwissProt ID
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Synonyms
SAP49, SF3B4, Splicing factor 3B subunit 4, Pre-mRNA-splicing factor SF3b 49 kDa subunit, Spliceosome-associated protein 49, SAP 49
Documentation
[1]. Das R, et al. Functional association of U2 snRNP with the ATP-independent spliceosomal complex E. Mol Cell. 2000 May;5(5):779-87. [Content Brief]
[2]. Will CL, et al. Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein. EMBO J. 2002 Sep 16;21(18):4978-88. [Content Brief]
[3]. Cretu C, et al. Molecular Architecture of SF3b and Structural Consequences of Its Cancer-Related Mutations. Mol Cell. 2016 Oct 20;64(2):307-319. [Content Brief]
[4]. Zhang Z, et al. Molecular architecture of the human 17S U2 snRNP. Nature. 2020 Jul;583(7815):310-313. [Content Brief]
[5]. Will CL, et al. The human 18S U11/U12 snRNP contains a set of novel proteins not found in the U2-dependent spliceosome. RNA. 2004 Jun;10(6):929-41. [Content Brief]
[6]. Bai R, et al. Structure of the activated human minor spliceosome. Science. 2021 Mar 19;371(6535):. [Content Brief]
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