PMEPA1 Antibody
(Synonyms: STAG1, TMEPAI, PMEPA1, Protein TMEPAI, Prostate transmembrane protein androgen induced 1, Solid tumor-associated 1 protein, Transmembrane prostate androgen-induced protein)PMEPA1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to PMEPA1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 |
Product Details
PMEPA1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to PMEPA1.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse
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Observed Molecular WeightObserved band size: 32 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 31 kDa
Synthetic peptide corresponding to the center region of human PMEPA1.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PMEPA1 functions as a negative regulator of TGF-beta signaling and thereby probably plays a role in cell proliferation, differentiation, apoptosis, motility, extracellular matrix production and immunosuppression. In the canonical TGF-beta pathway, ZFYVE9/SARA recruits the intracellular signal transducer and transcriptional modulators SMAD2 and SMAD3 to the TGF-beta receptor. Phosphorylated by the receptor, SMAD2 and SMAD3 then form a heteromeric complex with SMAD4 that translocates to the nucleus to regulate transcription. Through interaction with SMAD2 and SMAD3, LDLRAD4 may compete with ZFYVE9 and SMAD4 and prevent propagation of the intracellular signal. Also involved in down-regulation of the androgen receptor (AR), enhancing ubiquitination and proteasome-mediated degradation of AR, probably by recruiting NEDD4[1][2][3].
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Subcellular Localization
Early endosome membrane; Golgi apparatus membrane
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Expression
Tissue_Specificity: Highest expression in prostate. Also expressed in ovary.
Induction: Up-regulated by androgen and TGF-beta (at protein level). -
Isoforms & Post-Translational Modification
PMEPA1 has 3 isoforms, Q969W9-1: amino acid length is 287, molecular weight is 31609 Da (predicted); Q969W9-2: amino acid length is 252, molecular weight is 27900 Da (predicted); Q969W9-3: amino acid length is 237, molecular weight is 26201 Da (predicted).
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Subunit
Interacts with NEDD4 (via PPxY motifs).
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SwissProt ID
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Synonyms
STAG1, TMEPAI, PMEPA1, Protein TMEPAI, Prostate transmembrane protein androgen induced 1, Solid tumor-associated 1 protein, Transmembrane prostate androgen-induced protein
Documentation
[1]. Watanabe Y, et al. TMEPAI, a transmembrane TGF-beta-inducible protein, sequesters Smad proteins from active participation in TGF-beta signaling. Mol Cell. 2010 Jan 15;37(1):123-34. [Content Brief]
[2]. Nakano N, et al. C18 ORF1, a novel negative regulator of transforming growth factor-β signaling. J Biol Chem. 2014 May 2;289(18):12680-92. [Content Brief]
[3]. Li H, et al. A feedback loop between the androgen receptor and a NEDD4-binding protein, PMEPA1, in prostate cancer cells. J Biol Chem. 2008 Oct 24;283(43):28988-95. [Content Brief]
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