Lysophospholipase 1 Antibody (YA9935)
(Synonyms: APT1; hAPT1; LPL1; LYPLA1; Lysophospholipase 1; LYSOPLA; LysoPLA I)
Based on 1 Customer Validation
Lysophospholipase 1 Antibody (YA9935) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Lysophospholipase 1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, ICC/IF, IHC-P
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol, 0.05% Sodium Azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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|---|---|---|---|
| Dilution Ratio | 1:500-1000 | 1:50 | 1:50-200 |
Product Details
Lysophospholipase 1 Antibody (YA9935) is a Rabbit-derived and non-conjugated IgG Recombinant, Monoclonal antibody, targeting to Lysophospholipase 1.
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Host Rabbit
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 25 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 25 kDa
Recombinant protein within Human Lysophospholipase 1 aa 1-160 / 230.
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol, 0.05% Sodium Azide.
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Concentration
Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Verification Images
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Western blot analysis of extracts from Hela (lane2, 20μg), K562 (lane3, 20μg), THP-1 (lane4, 20μg) and Daudi (lane5, 20μg) using Lysophospholipase 1 Antibody (HY-P810677). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/500) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
Background
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Function
Lysophospholipase 1 acts as an acyl-protein thioesterase. Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS. Acts as a palmitoyl thioesterase that catalyzes depalmitoylation of proteins, such as ADRB2, KCNMA1 and SQSTM1. Acts as a negative regulator of autophagy by mediating palmitoylation of SQSTM1, decreasing affinity between SQSTM1 and ATG8 proteins and recruitment of ubiquitinated cargo proteins to autophagosomes. Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC). Also hydrolyzes lysophosphatidylethanolamine (lyso-PE), lysophosphatidylinositol (lyso-PI) and lysophosphatidylserine (lyso-PS) (By similarity). Has much higher thioesterase activity than lysophospholipase activity. Contributes to the production of lysophosphatidic acid (LPA) during blood coagulation by recognizing and cleaving plasma phospholipids to generate lysophospholipids which in turn act as substrates for ENPP2 to produce LPA[1][2][3][4][5][6].
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Subcellular Localization
Cytoplasm; Cell membrane; Nucleus membrane; Endoplasmic reticulum
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Expression
Tissue_Specificity: Platelets. -
Isoforms & Post-Translational Modification
Lysophospholipase 1 has 2 isoforms, O75608-1: amino acid length is 230, molecular weight is 24670 Da (predicted); O75608-2: amino acid length is 214, molecular weight is 22875 Da (predicted).
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Subunit
Homodimer
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SwissProt ID
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Synonyms
APT1; hAPT1; LPL1; LYPLA1; Lysophospholipase 1; LYSOPLA; LysoPLA I
Documentation
References
[1]. Hirano T, et al. Thioesterase activity and subcellular localization of acylprotein thioesterase 1/lysophospholipase 1. Biochim Biophys Acta. 2009 Aug;1791(8):797-805. [Content Brief]
[2]. Dekker FJ, et al. Small-molecule inhibition of APT1 affects Ras localization and signaling. Nat Chem Biol. 2010 Jun;6(6):449-56. [Content Brief]
[3]. Tian L, et al. Distinct acyl protein transferases and thioesterases control surface expression of calcium-activated potassium channels. J Biol Chem. 2012 Apr 27;287(18):14718-25. [Content Brief]
[4]. Adachi N, et al. S-Palmitoylation of a Novel Site in the β2-Adrenergic Receptor Associated with a Novel Intracellular Itinerary. J Biol Chem. 2016 Sep 16;291(38):20232-46. [Content Brief]
[5]. Huang X, et al. S-acylation of p62 promotes p62 droplet recruitment into autophagosomes in mammalian autophagy. Mol Cell. 2023 Oct 5;83(19):3485-3501.e11. [Content Brief]
[6]. Bolen AL, et al. The phospholipase A1 activity of lysophospholipase A-I links platelet activation to LPA production during blood coagulation. J Lipid Res. 2011 May;52(5):958-70. [Content Brief]
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