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Histone H3 (acetyl K27) Antibody (YA735)

(Synonyms: H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l)
2 Cited Publications
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Based on 2 publication(s) in Google Scholar

Histone H3 (acetyl K27) Antibody (YA735) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Histone H3 (acetyl K27).

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IHC-P, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Histone H3 (acetyl K27) Antibody (YA735)
  • Experimental Validation Results for Histone H3 (acetyl K27) Antibody (YA735)
  • Histone H3 (acetyl K27) Antibody (YA735) Product Image
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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:2000 1:100 1:600 1:500

Product Details

Description

Histone H3 (acetyl K27) Antibody (YA735) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Histone H3 (acetyl K27).

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 15 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 15 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Histone H3.AA range:20-40.

Sensitivity

Endogenous

Purification

Protein G affinity purified.

Conjugation

Non-conjugated

Modification

Acetylated

Isotype

IgG

RRID

AB_3102327

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Histone H3 (acetyl K27) Antibody (YA735)
    Western blot analysis of extracts from Hela (lane 1) and Jurkat (lane 2) using Histone H3 (acetyl K27) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Histone H3 (acetyl K27) Antibody (YA735)
    Flow cytometric analysis of 1X106 HeLa cells labeling Histone H3 (acetyl K27) Antibody (HY-P80169, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/500 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    Histone H3 core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling

  • Subcellular Localization

    Nucleus; Chromosome

  • Subunit

    The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with TONSL; CHAF1A; CHAF1B; MCM2 and DNAJC9 (PubMed:33857403). Interacts with NASP; NASP is a histone chaperone that stabilizes and maintains a soluble pool of Histone H3-H4 dimers (PubMed:22195965)

  • SwissProt ID

    P68431

  • Gene ID
    8350 [NCBI]
  • Synonyms

    H3FA, HIST1H3A, H3C2, H3FL, HIST1H3B, H3C3, H3FC HIST1H3C, H3C4, H3FB, HIST1H3D, H3C6, H3FD, HIST1H3E, H3C7, H3FI, HIST1H3F, H3C8, H3FH, HIST1H3G, H3C10, H3FK, HIST1H3H, H3C11, H3FF, HIST1H3I, H3C12, H3FJ, HIST1H3J, HIST1H3C, H3FC, H3C1, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

  • Research Field

    Epigenetics and Nuclear Signaling

Histone H3 (acetyl K27) Antibody (YA735) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 1) and Jurkat (lane 2) using Histone H3 (acetyl K27) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling Histone H3 (acetyl K27) Antibody (HY-P80169, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/500 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Histone H3 (acetyl K27) Antibody (YA735) Product Image
    1/16
  • Experimental Validation Results for Histone H3 (acetyl K27) Antibody (YA735)
  • Experimental Validation Results for Histone H3 (acetyl K27) Antibody (YA735)
  • Histone H3 (acetyl K27) Antibody (YA735) Product Image
  • Experimental Validation Results for Histone H3 (acetyl K27) Antibody (YA735)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 1) and Jurkat (lane 2) using Histone H3 (acetyl K27) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
  • Experimental Validation Results for Histone H3 (acetyl K27) Antibody (YA735)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling Histone H3 (acetyl K27) Antibody (HY-P80169, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/500 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Histone H3 (acetyl K27) Antibody (YA735)
  • Experimental Validation Results for Histone H3 (acetyl K27) Antibody (YA735)
  • Histone H3 (acetyl K27) Antibody (YA735) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 1) and Jurkat (lane 2) using Histone H3 (acetyl K27) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

  • Histone H3 (acetyl K27) Antibody (YA735) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling Histone H3 (acetyl K27) Antibody (HY-P80169, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/500 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Histone H3 (acetyl K27) Antibody (YA735) Product Image WB
  • Histone H3 (acetyl K27) Antibody (YA735) Product Image FC