Histone H3 (acetyl K27) Antibody (YA735)

Cat. No.: HY-P80169: Fiche technique COA
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Histone H3 (acetyl K27) Antibody (YA735) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Histone H3 (acetyl K27).

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Size	Stock	Quantité
20 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtenir un devis

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Histone H3 (acetyl K27) Antibody (YA735) Recommandations en vedette:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Description

Description

Histone H3 (acetyl K27) Antibody (YA735) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Histone H3 (acetyl K27).

Host

Mouse

Clonality

Monoclonal

Masse moléculaire

Predicted band size: 15 kDa;

Observed band size: 15 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P68431

Gene ID

8350 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Histone H3.AA range:20-40.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:600
FC FC: Flow Cytometry	1:500

Sensitivity	Endogenous	Pureté	Protein G affinity purified.
Conjugation	Non-conjugated	Modification	Acetylated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane 1) and Jurkat (lane 2) using Histone H3 (acetyl K27) antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

Background

Function：Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling

Subcellular Localization：Nucleus; Chromosome

Subunit：The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with TONSL; CHAF1A; CHAF1B; MCM2 and DNAJC9 (PubMed:33857403). Interacts with NASP; NASP is a histone chaperone that stabilizes and maintains a soluble pool of Histone H3-H4 dimers (PubMed:22195965)

RRID

AB_3102327

Database

Entrez Gene: 8350 Human ; 8351 Human ; 8352 Human ; 8353 Human ; 8354 Human ; 8355 Human ; 8356 Human ; 8357 Human ; 8358 Human ; 8968 Human ; 319152 Mouse ; 319153 Mouse ; 360198 Mouse ; 97908 Mouse ; 100364501 Rat 100365669

SwissProt: P68431 Human ; P84243 Human ; Q16695 Human ; Q6NXT2 Human ; Q71DI3 Human ; P68433 Mouse ; P84228 Mouse

OMIM: 601128 Human ; 602810 Human ; 602811 Human ; 602812 Human ; 602813 Human ; 602814 Human ; 602815 Human ; 602816 Human ; 602817 Human ; 602818 Human ; 602819 Human ; Human

Research Field

Epigenetics and Nuclear Signaling

Documentation

Select Batch:

Fiche technique (235 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Histone H3 (acetyl K27) Antibody (YA735) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Histone H3 (acetyl K27) Antibody (YA735)

Histone H3 (acetyl K27) Antibody (YA735) Recommandations en vedette:

Product Detail

Verification Image

Background

Description

Histone H3 (acetyl K27) Antibody (YA735) Related Classifications

Vos produits récemment consultés:

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