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Histone H3 (acetyl K18) Antibody (YA736)

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Histone H3 (acetyl K18) Antibody (YA736) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Histone H3 (acetyl K18).

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Rat

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Histone H3 (acetyl K18) Antibody (YA736)
  • Experimental Validation Results for Histone H3 (acetyl K18) Antibody (YA736)
  • Experimental Validation Results for Histone H3 (acetyl K18) Antibody (YA736)
  • Histone H3 (acetyl K18) Antibody (YA736) Product Image
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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:2000 1:500

Product Details

Description

Histone H3 (acetyl K18) Antibody (YA736) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Histone H3 (acetyl K18).

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Rat
  • Observed Molecular Weight
    Observed band size: 15 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 15 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Histone H3.AA range:1-50.

Sensitivity

Endogenous

Purification

Protein G affinity purified.

Conjugation

Non-conjugated

Modification

Acetylated

Isotype

IgG1

RRID

AB_3102842

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Histone H3 (acetyl K18) Antibody (YA736)
    Western blot analysis of extracts from C6 (lane 2, 20ug), C6 treated with 0.5μM Trichostatin A for 18 hours (lane3, 20μg) and C6 treated with 1μM Trichostatin A for 18 hours (lane4, 20μg) using Histone H3 (acetyl K18) Antibody (HY-P80168). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Histone H3 (acetyl K18) Antibody (YA736)
    Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Histone H3 (acetyl K18) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80168, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Histone H3 (acetyl K18) Antibody (YA736)
    Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Histone H3 (acetyl K18) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80168, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Histone H3 core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling

  • Subcellular Localization

    Nucleus; Chromosome

  • Subunit

    The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with TONSL; CHAF1A; CHAF1B; MCM2 and DNAJC9 (PubMed:33857403). Interacts with NASP; NASP is a histone chaperone that stabilizes and maintains a soluble pool of Histone H3-H4 dimers (PubMed:22195965)

  • SwissProt ID

    P68431

  • Gene ID
    8350 [NCBI]
  • Research Field

    Epigenetics and Nuclear Signaling

Histone H3 (acetyl K18) Antibody (YA736) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane 2, 20ug), C6 treated with 0.5μM Trichostatin A for 18 hours (lane3, 20μg) and C6 treated with 1μM Trichostatin A for 18 hours (lane4, 20μg) using Histone H3 (acetyl K18) Antibody (HY-P80168). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Histone H3 (acetyl K18) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80168, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Histone H3 (acetyl K18) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80168, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Histone H3 (acetyl K18) Antibody (YA736) Product Image
    1/16
  • Experimental Validation Results for Histone H3 (acetyl K18) Antibody (YA736)
  • Experimental Validation Results for Histone H3 (acetyl K18) Antibody (YA736)
  • Experimental Validation Results for Histone H3 (acetyl K18) Antibody (YA736)
  • Histone H3 (acetyl K18) Antibody (YA736) Product Image
  • Experimental Validation Results for Histone H3 (acetyl K18) Antibody (YA736)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from C6 (lane 2, 20ug), C6 treated with 0.5μM Trichostatin A for 18 hours (lane3, 20μg) and C6 treated with 1μM Trichostatin A for 18 hours (lane4, 20μg) using Histone H3 (acetyl K18) Antibody (HY-P80168). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Histone H3 (acetyl K18) Antibody (YA736)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Histone H3 (acetyl K18) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80168, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Histone H3 (acetyl K18) Antibody (YA736)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Histone H3 (acetyl K18) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80168, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Histone H3 (acetyl K18) Antibody (YA736)
  • Experimental Validation Results for Histone H3 (acetyl K18) Antibody (YA736)
  • Experimental Validation Results for Histone H3 (acetyl K18) Antibody (YA736)
  • Histone H3 (acetyl K18) Antibody (YA736) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from C6 (lane 2, 20ug), C6 treated with 0.5μM Trichostatin A for 18 hours (lane3, 20μg) and C6 treated with 1μM Trichostatin A for 18 hours (lane4, 20μg) using Histone H3 (acetyl K18) Antibody (HY-P80168). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004,1/10,000) was used for 1 hour at room temperature.

  • Histone H3 (acetyl K18) Antibody (YA736) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Histone H3 (acetyl K18) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80168, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Histone H3 (acetyl K18) Antibody (YA736) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue using Histone H3 (acetyl K18) Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80168, 1/500) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Histone H3 (acetyl K18) Antibody (YA736) Product Image WB
  • Histone H3 (acetyl K18) Antibody (YA736) Product Image IHC-P
  • Histone H3 (acetyl K18) Antibody (YA736) Product Image IHC-P