IQGAP1 Antibody (YA2388)

Cat. No.: HY-P82643: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

IQGAP1 Antibody (YA2388) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IQGAP1.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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IQGAP1 Antibody (YA2388) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

IQGAP1 Antibody (YA2388) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IQGAP1.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 189 kDa;

Observed band size: 180 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P46940

Gene ID

8826 [NCBI]

Immunogen

A synthesized peptide derived from human IQGAP1 aa1-58/1657.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
FC FC: Flow Cytometry	1:50-1:100

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in rabbit IgG in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

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Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), HUVEC (lane 2, 20 μg), Jurkat (lane 3, 20 μg), 293T (lane 4, 20 μg), 293 (lane 5, 20 μg), and Mouse thymus (lane 6, 20 μg) using IQGAP1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. Recruited to the cell cortex by interaction with ILK which allows it to cooperate with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane (By similarity). Binds to activated CDC42 but does not stimulate its GTPase activity. Associates with calmodulin. May promote neurite outgrowth (PubMed:15695813). May play a possible role in cell cycle regulation by contributing to cell cycle progression after DNA replication arrest (PubMed:20883816)

Subcellular Localization：Cell membrane; Nucleus; Cytoplasm; Cytoplasm, cell cortex; Apical cell membrane; Basolateral cell membrane

Expression：
Tissue_specificity:Expressed in the placenta, lung, and kidney (PubMed:8051149) . A lower level expression is seen in the heart, liver, skeletal muscle and pancreas (PubMed:8051149)

Subunit：Interacts with CDC42; the interaction is demonstrated with IQGAP1 in GTP-bound and in nucleotide-free state (PubMed:15355962). Interacts with RAC1 (PubMed:15355962). Does not interact with RHOA. Interacts with TSG101 (PubMed:17853893). Interacts with PAK6 (PubMed:18642328). Interacts with TMEM14B; this interaction increases IQGAP1 phosphorylation and induces its nuclear translocation (PubMed:29033352). Interacts with SASH1 (PubMed:23333244). Interacts with PJVK (By similarity). Interacts with SLC26A4; this interaction enhances the chloride-bicarbonate exchange activity of SLC26A4 (PubMed:35601831). Interacts with SVEP1 (By similarity). Interacts with ILK; the interaction is required for localization of IQGAP to the cell cortex (By similarity)

RRID

AB_3104743

Database

Entrez Gene: 8826 Human ; 29875 Mouse ;

SwissProt: P46940 Human ; Q9JKF1 Mouse ;

OMIM: 603379 Human

Research Field

Signal Transduction

Synonyms

IQGAP1; KIAA0051; Ras GTPase-activating-like protein IQGAP1; p195

Documentation

Select Batch:

Data Sheet (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

IQGAP1 Antibody (YA2388) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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IQGAP1 Antibody (YA2388)

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