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IQGAP1 Antibody (YA2388)

(Synonyms: IQGAP1; KIAA0051; Ras GTPase-activating-like protein IQGAP1; p195)
Customer Review

Based on 1 Customer Validation

IQGAP1 Antibody (YA2388) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IQGAP1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, FC

  • Reactivity :

    Human, Mouse

  • Formulation:

    Supplied in rabbit IgG in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
ALL WB IHC-P FC
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • IQGAP1 Antibody (YA2388) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:200 1:50-1:100

Product Details

Description

IQGAP1 Antibody (YA2388) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IQGAP1.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse
  • Observed Molecular Weight
    Observed band size: 180 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 189 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human IQGAP1 aa1-58/1657.

Sensitivity

Endogenous

Purification

Affinity Chromatography

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3104743

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 10 mM PBS, pH 7.4, 150 mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
    Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), HUVEC (lane 2, 20 μg), Jurkat (lane 3, 20 μg), 293T (lane 4, 20 μg), 293 (lane 5, 20 μg), and Mouse thymus (lane 6, 20 μg) using IQGAP1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
    Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
    Immunohistochemical analysis of paraffin-embedded human placenta tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
    Immunohistochemical analysis of paraffin-embedded human testis tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
    Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
    Immunohistochemical analysis of paraffin-embedded mouse rectum tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
    Flow cytometric analysis of 1X106 HeLa cells labeling IQGAP1 Antibody (HY-P82643, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

  • Function

    IQGAP1 plays a crucial role in regulating the dynamics and assembly of the actin cytoskeleton. Recruited to the cell cortex by interaction with ILK which allows it to cooperate with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane. Binds to activated CDC42 but does not stimulate its GTPase activity. Associates with calmodulin. May promote neurite outgrowth. May play a possible role in cell cycle regulation by contributing to cell cycle progression after DNA replication arrest[1][2].

  • Subcellular Localization

    Cell membrane; Nucleus; Cytoplasm; Cytoplasm, cell cortex; Apical cell membrane; Basolateral cell membrane

  • Expression


    Tissue_specificity:Expressed in the placenta, lung, and kidney (PubMed:8051149) . A lower level expression is seen in the heart, liver, skeletal muscle and pancreas (PubMed:8051149)

  • Subunit

    Interacts with CDC42; the interaction is demonstrated with IQGAP1 in GTP-bound and in nucleotide-free state (PubMed:15355962). Interacts with RAC1 (PubMed:15355962). Does not interact with RHOA. Interacts with TSG101 (PubMed:17853893). Interacts with PAK6 (PubMed:18642328). Interacts with TMEM14B; this interaction increases IQGAP1 phosphorylation and induces its nuclear translocation (PubMed:29033352). Interacts with SASH1 (PubMed:23333244). Interacts with PJVK (By similarity). Interacts with SLC26A4; this interaction enhances the chloride-bicarbonate exchange activity of SLC26A4 (PubMed:35601831). Interacts with SVEP1 (By similarity). Interacts with ILK; the interaction is required for localization of IQGAP to the cell cortex (By similarity)

  • SwissProt ID

    P46940

  • Gene ID
    8826 [NCBI]
  • Synonyms

    IQGAP1; KIAA0051; Ras GTPase-activating-like protein IQGAP1; p195

  • Research Field

    Signal Transduction

IQGAP1 Antibody (YA2388) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), HUVEC (lane 2, 20 μg), Jurkat (lane 3, 20 μg), 293T (lane 4, 20 μg), 293 (lane 5, 20 μg), and Mouse thymus (lane 6, 20 μg) using IQGAP1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lung tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse colon tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse rectum tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling IQGAP1 Antibody (HY-P82643, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • IQGAP1 Antibody (YA2388) Product Image
    1/16
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • IQGAP1 Antibody (YA2388) Product Image
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
    1/1
    MedchemExpress Validation
     Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), HUVEC (lane 2, 20 μg), Jurkat (lane 3, 20 μg), 293T (lane 4, 20 μg), 293 (lane 5, 20 μg), and Mouse thymus (lane 6, 20 μg) using IQGAP1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human placenta tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human testis tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse lung tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse colon tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse rectum tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling IQGAP1 Antibody (HY-P82643, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • Experimental Validation Results for IQGAP1 Antibody (YA2388)
  • IQGAP1 Antibody (YA2388) Product Image
    MedchemExpress Validation

    Western blot analysis was performed on extracts from Hela (lane 1, 20 μg), HUVEC (lane 2, 20 μg), Jurkat (lane 3, 20 μg), 293T (lane 4, 20 μg), 293 (lane 5, 20 μg), and Mouse thymus (lane 6, 20 μg) using IQGAP1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

  • IQGAP1 Antibody (YA2388) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IQGAP1 Antibody (YA2388) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IQGAP1 Antibody (YA2388) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human testis tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IQGAP1 Antibody (YA2388) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IQGAP1 Antibody (YA2388) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IQGAP1 Antibody (YA2388) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IQGAP1 Antibody (YA2388) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse rectum tissue using IQGAP1 Antibody (HY-P82643, 1/100). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • IQGAP1 Antibody (YA2388) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling IQGAP1 Antibody (HY-P82643, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/100 dilution for an hour at 4℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • IQGAP1 Antibody (YA2388) Product Image WB
  • IQGAP1 Antibody (YA2388) Product Image IHC-P
  • IQGAP1 Antibody (YA2388) Product Image IHC-P
  • IQGAP1 Antibody (YA2388) Product Image IHC-P
  • IQGAP1 Antibody (YA2388) Product Image IHC-P
  • IQGAP1 Antibody (YA2388) Product Image IHC-P
  • IQGAP1 Antibody (YA2388) Product Image IHC-P
  • IQGAP1 Antibody (YA2388) Product Image IHC-P
  • IQGAP1 Antibody (YA2388) Product Image FC