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Mitofusin-2 Antibody (YA6027)

(Synonyms: MFN2; CPRP1; KIAA0214; Mitofusin-2; Transmembrane GTPase MFN2)
Customer Review

Based on 1 Customer Validation

Mitofusin-2 Antibody (YA6027) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Mitofusin-2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for Mitofusin-2 Antibody (YA6027)
  • Experimental Validation Results for Mitofusin-2 Antibody (YA6027)
  • Experimental Validation Results for Mitofusin-2 Antibody (YA6027)
  • Mitofusin-2 Antibody (YA6027) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Data Download
Related Products:
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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:200-1:1000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

Mitofusin-2 Antibody (YA6027) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Mitofusin-2.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 86 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 86 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719121

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Mitofusin-2 Antibody (YA6027)
    Western blot analysis of extracts from HEK293 (lane 2(20μg), PC-3 (lane 3(20μg), Raji (lane 4(20μg) and HL-60 (lane 5(20μg) using Mitofusin-2 Antibody (HY-P86335) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Mitofusin-2 Antibody (YA6027)
    Immunocytochemistry analysis of Hela cells labeling Mitofusin-2 with Mitofusin-2 Antibody (HY-P86335) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Mitofusin-2 Antibody (HY-P86335) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Mitofusin-2 Antibody (YA6027)
    Immunocytochemistry analysis of PC-3 cells labeling Mitofusin-2 with Mitofusin-2 Antibody (HY-P86335) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Mitofusin-2 Antibody (HY-P86335) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Mitofusin-2 is a Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks. Membrane clustering requires GTPase activity and may involve a major rearrangement of the coiled coil domains (Probable). Plays a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes. Plays an important role in the regulation of vascular smooth muscle cell proliferation. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). Is required for PRKN recruitment to dysfunctional mitochondria. Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress. Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions[1][2][3][4][5][6].

  • Subcellular Localization

    Mitochondrion outer membrane; Multi-pass membrane protein

  • Expression


    Tissue_specificity:It is widely distributed; its expression level is low. It is highly expressed in the heart and kidneys.

  • Isoforms & Post-Translational Modification

    O95140 has 2 isomers: O95140-1: 86402 Da (predicted); O95140-2: 50041 Da (predicted).
    Phosphorylated by PINK1;Ubiquitinated by non-degradative ubiquitin by PRKN, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion (PubMed:23620051). Ubiquitinated by HUWE1 when dietary stearate (C18:0) levels are low; ubiquitination inhibits mitochondrial fusion (PubMed:26214738, PubMed:30217973)

  • Subunit

    Forms homomultimers and heteromultimers with MFN1 (PubMed:26085578). Interacts with STOML2; may form heterooligomers (PubMed:17121834). Interacts (phosphorylated) with PRKN (PubMed:23620051).

  • SwissProt ID

    O95140

  • Gene ID
    9927 [NCBI]
  • Synonyms

    MFN2; CPRP1; KIAA0214; Mitofusin-2; Transmembrane GTPase MFN2

Mitofusin-2 Antibody (YA6027) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2(20μg), PC-3 (lane 3(20μg), Raji (lane 4(20μg) and HL-60 (lane 5(20μg) using Mitofusin-2 Antibody (HY-P86335) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Mitofusin-2 with Mitofusin-2 Antibody (HY-P86335) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Mitofusin-2 Antibody (HY-P86335) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling Mitofusin-2 with Mitofusin-2 Antibody (HY-P86335) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Mitofusin-2 Antibody (HY-P86335) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Mitofusin-2 Antibody (YA6027) Product Image
    1/16
  • Experimental Validation Results for Mitofusin-2 Antibody (YA6027)
  • Experimental Validation Results for Mitofusin-2 Antibody (YA6027)
  • Experimental Validation Results for Mitofusin-2 Antibody (YA6027)
  • Mitofusin-2 Antibody (YA6027) Product Image
  • Experimental Validation Results for Mitofusin-2 Antibody (YA6027)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HEK293 (lane 2(20μg), PC-3 (lane 3(20μg), Raji (lane 4(20μg) and HL-60 (lane 5(20μg) using Mitofusin-2 Antibody (HY-P86335) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Mitofusin-2 Antibody (YA6027)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling Mitofusin-2 with Mitofusin-2 Antibody (HY-P86335) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Mitofusin-2 Antibody (HY-P86335) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Mitofusin-2 Antibody (YA6027)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of PC-3 cells labeling Mitofusin-2 with Mitofusin-2 Antibody (HY-P86335) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Mitofusin-2 Antibody (HY-P86335) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Mitofusin-2 Antibody (YA6027)
  • Experimental Validation Results for Mitofusin-2 Antibody (YA6027)
  • Experimental Validation Results for Mitofusin-2 Antibody (YA6027)
  • Mitofusin-2 Antibody (YA6027) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HEK293 (lane 2(20μg), PC-3 (lane 3(20μg), Raji (lane 4(20μg) and HL-60 (lane 5(20μg) using Mitofusin-2 Antibody (HY-P86335) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000)and Loading control antibody (Beta Actin, HY-P80993, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Mitofusin-2 Antibody (YA6027) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling Mitofusin-2 with Mitofusin-2 Antibody (HY-P86335) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Mitofusin-2 Antibody (HY-P86335) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Mitofusin-2 Antibody (YA6027) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of PC-3 cells labeling Mitofusin-2 with Mitofusin-2 Antibody (HY-P86335) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Mitofusin-2 Antibody (HY-P86335) at 1/300 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Mitofusin-2 Antibody (YA6027) Product Image WB
  • Mitofusin-2 Antibody (YA6027) Product Image ICC
  • Mitofusin-2 Antibody (YA6027) Product Image ICC