ARFIP1 Antibody
(Synonyms: Arfaptin-1, ADP-ribosylation factor-interacting protein 1, ARFIP1)ARFIP1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ARFIP1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:100-500 |
Product Details
ARFIP1 Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to ARFIP1.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 42 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 41 kDa
Synthetic peptide corresponding to the C-term region of human ARFIP1.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
ARFIP1 plays a role in controlling biogenesis of secretory granules at the trans-Golgi network. Mechanistically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold. Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs. In turn, ARFs induce fission. Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells[1][2].
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Subcellular Localization
Golgi apparatus; Golgi apparatus, trans-Golgi network membrane
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Expression
Tissue_Specificity: Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart. -
Isoforms & Post-Translational Modification
ARFIP1 has 3 isoforms, P53367-1: amino acid length is 373, molecular weight is 41738 Da (predicted); P53367-2: amino acid length is 341, molecular weight is 38599 Da (predicted); P53367-3: amino acid length is 193, molecular weight is 22055 Da (predicted).
Phosphorylated by PRKD1; phosphorylation delocalizes ARFIP1 from the Golgi and disrupts its ability to inhibit the activity of ADP-ribosylation factor, an important component of the vesicle scission machinery. -
Subunit
Forms homodimers or heterodimers with ARFIP2.
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SwissProt ID
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Synonyms
Arfaptin-1, ADP-ribosylation factor-interacting protein 1, ARFIP1
Documentation
[1]. Gehart H, et al. The BAR domain protein Arfaptin-1 controls secretory granule biogenesis at the trans-Golgi network. Dev Cell. 2012 Oct 16;23(4):756-68. [Content Brief]
[2]. Kanoh H, et al. Arfaptin 1, a putative cytosolic target protein of ADP-ribosylation factor, is recruited to Golgi membranes. J Biol Chem. 1997 Feb 28;272(9):5421-9. [Content Brief]
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