PTGIS Antibody
(Synonyms: Cyp8, Prostacyclin synthase, Hydroperoxy icosatetraenoate dehydratase, Prostaglandin I2 synthase)PTGIS Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to PTGIS.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
WB
WB: Western Blot
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IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
|---|---|---|---|
| Dilution Ratio | 1:1000-2000 | 1:100-200 | 1:50-200 |
Product Details
PTGIS Antibody is a Rabbit-derived and non-conjugated IgG Polyclonal antibody, targeting to PTGIS.
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Host Rabbit
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Clonality Polyclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 57 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 57 kDa
Synthetic peptide corresponding to the C-term region of human PTGIS.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH 7.4), containing 30% glycerol, and 0.01% sodium azide.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
PTGIS catalyzes the biosynthesis and metabolism of eicosanoids. Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2), a potent mediator of vasodilation and inhibitor of platelet aggregation. Additionally, displays dehydratase activity, toward hydroperoxyeicosatetraenoates (HPETEs), especially toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE)[1][2][3][4][5].
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Subcellular Localization
Endoplasmic reticulum membrane
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Expression
Tissue_Specificity: Widely expressed; particularly abundant in ovary, heart, skeletal muscle, lung and prostate. -
Isoforms & Post-Translational Modification
PTGIS has an amino acid length of 500, molecular weight is 57104 Da.
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SwissProt ID
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Synonyms
Cyp8, Prostacyclin synthase, Hydroperoxy icosatetraenoate dehydratase, Prostaglandin I2 synthase
Documentation
[1]. Nakayama T, et al. Splicing mutation of the prostacyclin synthase gene in a family associated with hypertension. Biochem Biophys Res Commun. 2002 Oct 11;297(5):1135-9. [Content Brief]
[2]. Wada M, et al. Purification and characterization of recombinant human prostacyclin synthase. J Biochem. 2004 Apr;135(4):455-63. [Content Brief]
[3]. Li YC, et al. Structures of prostacyclin synthase and its complexes with substrate analog and inhibitor reveal a ligand-specific heme conformation change. J Biol Chem. 2008 Feb 1;283(5):2917-26. [Content Brief]
[4]. Cho SA, et al. Analysis of genetic polymorphism and biochemical characterization of a functionally decreased variant in prostacyclin synthase gene (CYP8A1) in humans. Arch Biochem Biophys. 2015 Mar 1;569:10-8. [Content Brief]
[5]. Yeh HC, et al. Reaction mechanisms of 15-hydroperoxyeicosatetraenoic acid catalyzed by human prostacyclin and thromboxane synthases. Arch Biochem Biophys. 2007 May 15;461(2):159-68. [Content Brief]
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