p16 ARC Antibody (YA1854)(PBS only)

Cat. No.: HY-P82109A: Data Sheet User Guide for Antibodies
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p16 ARC Antibody (YA1854) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to p16 ARC.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

p16 ARC Antibody (YA1854) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to p16 ARC.

Host

Rabbit

Molecular Weight

Predicted band size: 16 kDa;

Observed band size: 16 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

O15511

Gene ID

10092 [NCBI]

Immunogen

A synthetic peptide of human p16 ARC aa2-20.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:20

Purity	Affinity Purified	Conjugation	Non-conjugated
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947)

Subcellular Localization：Cytoplasm, cytoskeleton; Cell projection; Nucleus

Isoforms & Post-Translational Modification：O15511 has 2 isomers: O15511-1: 16320 Da (predicted); O15511-2: 16631 Da (predicted).
Polyubiquitinated by RNF128 with 'Lys-63'-linked chains, leading to proteasomal degradation

Subunit：Component of the Arp2/3 complex composed of ACTR2/ARP2, ACTR3/ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC

Research Field

Signal Transduction

Synonyms

p16 Arc; ARPC5; ARC16; Arp2/3 protein complex subunit p16

Documentation

User Guide for Antibodies (1077 KB)