RBPMS Antibody (YA7747)

(Synonyms: HERMES)
Customer Review

Based on 1 Customer Validation

RBPMS Antibody (YA7747) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RBPMS.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Conjugation:
    Non-conjugated

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
Dilution Ratio 1:2000-3000 1:150-500 1:100

Product Details

Description

RBPMS Antibody (YA7747) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to RBPMS.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Calculated Molecular Weight Predicted band size: 24.1 kDa
Immunogen

Full length human recombinant protein of human RBPMS produced in E.coli.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Spplied in PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for RBPMS Antibody (YA7747)
    Immunohistochemical analysis of paraffin-embedded rat kidney tissue using RBPMS Antibody (HY-P88063, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for RBPMS Antibody (YA7747)
    Immunohistochemical analysis of paraffin-embedded rat stomach tissue using RBPMS Antibody (HY-P88063, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for RBPMS Antibody (YA7747)
    Immunohistochemical analysis of paraffin-embedded rat rectum tissue using RBPMS Antibody (HY-P88063, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for RBPMS Antibody (YA7747)
    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using RBPMS Antibody (HY-P88063, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for RBPMS Antibody (YA7747)
    Immunohistochemical analysis of paraffin-embedded rat testis tissue using RBPMS Antibody (HY-P88063, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for RBPMS Antibody (YA7747)
    Immunohistochemical analysis of paraffin-embedded human salivary gland tissue using RBPMS Antibody (HY-P88063, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for RBPMS Antibody (YA7747)
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using RBPMS Antibody (HY-P88063, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for RBPMS Antibody (YA7747)
    Immunohistochemical analysis of paraffin-embedded human small intestine tissue using RBPMS Antibody (HY-P88063, 1/500). The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 8 minutes. The tissues were blocked with quick block buffer for 0.5 hours at room temperature, washed with PBS and PBST, and then incubated with the primary antibody overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    RBPMS is a RNA binding protein that mediates the regulation of pre-mRNA alternative splicing (AS). Acts either as activator (FLNB, HSPG2, LIPA1, MYOCD, PTPRF and PPFIBP1) or repressor (TPM1, ACTN1, ITGA7, PIEZO1, LSM14B, MBNL1 and MBML2) of splicing events on specific pre-mRNA targets. Together with RNA binding proteins RBFOX2 and MBNL1/2, activates a splicing program associated with differentiated contractile vascular smooth muscle cells (SMC) by regulating AS of numerous pre-mRNA involved in actin cytoskeleton and focal adhesion machineries, suggesting a role in promoting a cell differentiated state. Binds to introns, exons and 3'-UTR associated with tandem CAC trinucleotide motifs separated by a variable spacer region, at a minimum as a dimer. The minimal length of RNA required for RBPMS-binding tandem CAC motifs is 15 nt, with spacing ranging from 1 to 9 nt. Can also bind to CA dinucleotide repeats. Mediates repression of TPM1 exon 3 by binding to CAC tandem repeats in the flanking intronic regions, followed by higher-order oligomerization and heterotypic interactions with other splicing regulators including MBNL1 and RBFOX2, which prevents assembly of ATP-dependent splicing complexes|Acts as a regulator of pre-mRNA alternative splicing (AS). Binds mRNA. Regulates AS of ACTN1, FLNB, although with lower efficiency than isoform A / RBPMSA. Acts as coactivator of SMAD transcriptional activity in a TGFB1-dependent manner, possibly through increased phosphorylation of SMAD2 and SMAD3 at the C-terminal SSXS regions and promotion of the nuclear accumulation of SMAD proteins[1][2][3].

  • Subcellular Localization

    Nucleus,Cytoplasm,Cytoplasm, Stress granule,Cytoplasm, P-body

  • Expression


    Tissue_Specificity: Ubiquitously expressed, at various levels depending on the isoform and the tissue (PubMed:8855282). Strongly expressed in the heart, prostate, small intestine, large intestine, and ovary; moderately expressed in the placenta, lung, liver, kidney, pancreas, and testis; and poorly expressed in the skeletal muscle, spleen, thymus and peripheral leukocytes (PubMed:8855282)

  • Isoforms & Post-Translational Modification

    Q93062 has 5 isomers: Q93062-1: 21802 Da (predicted); Q93062-2: 22416 Da (predicted); Q93062-3: 24277 Da (predicted); Q93062-4: 15794 Da (predicted); Q93062-5: 17022 Da (predicted).

  • Subunit

    Homodimer; each protein chain binds one RNA molecule via the external surface of the homodimer (PubMed:26347403)

  • SwissProt ID

    Q93062

  • Synonyms

    HERMES

RBPMS Antibody (YA7747) Related Classifications

MOQ
Minimum order quantity
100 mg

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