SLUG Antibody (YA5060)

Cat. No.: HY-P85368: Data Sheet User Guide for Antibodies
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SLUG Antibody (YA5060) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to SLUG.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

SLUG Antibody (YA5060) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to SLUG.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 30 kDa;

Species Reactivity

Human

SwissProt ID

O43623

Gene ID

6591 [NCBI]

Immunogen

Purified recombinant fragment of human SLUG expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:2000
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells (By similarity). Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis

Subcellular Localization：Nucleus; Cytoplasm

Expression：
Tissue_specificity:It is expressed in most adult tissues, including the spleen, thymus, prostate, testes, ovaries, small intestine, colon, heart, brain, placenta, lungs, liver, skeletal muscle, kidneys, and pancreas. It is not detected in peripheral blood leukocytes. It is expressed in all layers of the dermis and epidermis, with higher expression levels in the basal layer (protein level) . It is expressed in osteoblasts (protein level) . It is expressed in mesenchymal stem cells (protein level) . It is expressed in breast tumor cells (protein level) .

Subunit：Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains) (By similarity). Interacts (via zinc fingers) with KPNA2, KPNB1, and TNPO1. May interact (via zinc fingers) with IPO7

Synonyms

SNAI2; SLUG; SLUGH; Zinc finger protein SNAI2; Neural crest transcription factor Slug; Protein snail homolog 2

Documentation

User Guide for Antibodies (1077 KB)