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Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616)

(Synonyms: ACH4; AChR; Acra4; BFNC; CHRNA4; EBN; EBN1; NACHR; NACRA4)
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Based on 1 Customer Validation

Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nicotinic Acetylcholine Receptor alpha 4.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616)
  • Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
Dilution Ratio 1:500-1:1000

Product Details

Description

Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nicotinic Acetylcholine Receptor alpha 4.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 70 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 70 kDa
Species Reactivity Database
Immunogen

A synthesized peptide derived from human ACHA4 aa540-600/627.

Sensitivity

Endogenous

Purification

Affinity Purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616)
    Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2), Mouse brain (lane 3), Ramos (lane 4) and HeLa (lane 5) using Nicotinic Acetylcholine Receptor alpha 4 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Nicotinic Acetylcholine Receptor alpha 4 component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators. CHRNA4 forms heteropentameric neuronal acetylcholine receptors with CHRNB2 and CHRNB4, as well as CHRNA5 and CHRNB3 as accesory subunits. Is the most abundant nAChR subtype expressed in the central nervous system. Found in two major stoichiometric forms,(CHRNA4)3:(CHRNB2)2 and (CHRNA4)2:(CHRNB2)3, the two stoichiometric forms differ in their unitary conductance, calcium permeability, ACh sensitivity and potentiation by divalent cation. Involved in the modulation of calcium-dependent signaling pathways, influences the release of neurotransmitters, including dopamine, glutamate and GABA[1][2][3][4][5].

  • Subcellular Localization

    Synaptic cell membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein

  • Isoforms & Post-Translational Modification

    P43681 has 2 isomers: P43681-1: 69957 Da (predicted); P43681-2: 60994 Da (predicted).

  • Subunit

    Neuronal AChR is composed of two different types of subunits: alpha and beta (PubMed:22361591). CHRNA4 forms heteropentameric neuronal acetylcholine receptors with CHRNB2 and CHRNB4, as well as CHRNA5 and CHRNB3 as accesory subunits (PubMed:20881005, PubMed:22361591, PubMed:27698419, PubMed:29720657, PubMed:38454578). Found in two major stoichiometric forms, LS (low agonist sensitivity): (CHRNA4)3:(CHRNB2)2 and HS (high agonist sensitivity): (CHRNA4)2:(CHRNB2)3, the two stoichiometric forms differ in their unitary conductance, calcium permeability, ACh sensitivity and potentiation by divalent cation (PubMed:12527804, PubMed:17132685, PubMed:22361591, PubMed:27698419, PubMed:29720657, PubMed:38454578). Cells produce predominantly an (CHRNA4)3:(CHRNB2)2 nAChR. The (CHRNA4)2:(CHRNB2)3 expression is selectively up-regulated by nicotine and has lower single channel conductance and calcium permeability (PubMed:17132685). In the striatum, also forms CHRNA4:CHRNA6:CHRNB2 complexes (By similarity). Also found in the stoichiometric form: (CHRNA4:CHRNB2)2:CHRNB3 (PubMed:16835356). Interacts with RIC3; which is required for proper folding and assembly (PubMed:16120769). Interacts with LYPD6 (PubMed:27344019)

  • SwissProt ID

    P43681

  • Gene ID
    1137 [NCBI]
  • Synonyms

    ACH4; AChR; Acra4; BFNC; CHRNA4; EBN; EBN1; NACHR; NACRA4

  • Research Field

    -

Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2), Mouse brain (lane 3), Ramos (lane 4) and HeLa (lane 5) using Nicotinic Acetylcholine Receptor alpha 4 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616) Product Image
    1/16
  • Experimental Validation Results for Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616)
  • Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616) Product Image
  • Experimental Validation Results for Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2), Mouse brain (lane 3), Ramos (lane 4) and HeLa (lane 5) using Nicotinic Acetylcholine Receptor alpha 4 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616)
  • Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from SH-SY5Y (lane 2), Mouse brain (lane 3), Ramos (lane 4) and HeLa (lane 5) using Nicotinic Acetylcholine Receptor alpha 4 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616) Product Image WB