2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies Recombinant Antibodies
  5. Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616)

Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616)

Cat. No.: HY-P82871
COA
SDS
User Guide for Antibodies Technical Support

Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nicotinic Acetylcholine Receptor alpha 4.

For research use only. We do not sell to patients.

Size Price Stock Quantity
20 μL In-stock
50 μL In-stock
100 μL In-stock
250 μL   Get quote  
or Bulk Inquiry

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Background

  • Documentation

Description

Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Nicotinic Acetylcholine Receptor alpha 4.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Molecular Weight
Predicted band size: 70 kDa;
Observed band size: 70 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthesized peptide derived from human ACHA4 aa540-600/627.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Purity Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Solution
Formulation

Supplied in rabbit IgG in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.
Background
Function：Component of neuronal acetylcholine receptors (nAChRs) that function as pentameric, ligand-gated cation channels with high calcium permeability among other activities. nAChRs are excitatory neurotrasnmitter receptors formed by a collection of nAChR subunits known to mediate synaptic transmission in the nervous system and the neuromuscular junction. Each nAchR subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, cation permeability, and binding to allosteric modulators (PubMed:22361591, PubMed:27698419, PubMed:29720657, PubMed:38454578). CHRNA4 forms heteropentameric neuronal acetylcholine receptors with CHRNB2 and CHRNB4, as well as CHRNA5 and CHRNB3 as accesory subunits. Is the most abundant nAChR subtype expressed in the central nervous system (PubMed:16835356, PubMed:22361591, PubMed:27698419, PubMed:29720657, PubMed:38454578). Found in two major stoichiometric forms,(CHRNA4)3:(CHRNB2)2 and (CHRNA4)2:(CHRNB2)3, the two stoichiometric forms differ in their unitary conductance, calcium permeability, ACh sensitivity and potentiation by divalent cation (PubMed:27698419, PubMed:29720657, PubMed:38454578). Involved in the modulation of calcium-dependent signaling pathways, influences the release of neurotransmitters, including dopamine, glutamate and GABA (By similarity)
Subcellular Localization：Synaptic cell membrane; Multi-pass membrane protein; Cell membrane; Multi-pass membrane protein
Isoforms & Post-Translational Modification：P43681 has 2 isomers: P43681-1: 69957 Da (predicted); P43681-2: 60994 Da (predicted).
Subunit：Neuronal AChR is composed of two different types of subunits: alpha and beta (PubMed:22361591). CHRNA4 forms heteropentameric neuronal acetylcholine receptors with CHRNB2 and CHRNB4, as well as CHRNA5 and CHRNB3 as accesory subunits (PubMed:20881005, PubMed:22361591, PubMed:27698419, PubMed:29720657, PubMed:38454578). Found in two major stoichiometric forms, LS (low agonist sensitivity): (CHRNA4)3:(CHRNB2)2 and HS (high agonist sensitivity): (CHRNA4)2:(CHRNB2)3, the two stoichiometric forms differ in their unitary conductance, calcium permeability, ACh sensitivity and potentiation by divalent cation (PubMed:12527804, PubMed:17132685, PubMed:22361591, PubMed:27698419, PubMed:29720657, PubMed:38454578). Cells produce predominantly an (CHRNA4)3:(CHRNB2)2 nAChR. The (CHRNA4)2:(CHRNB2)3 expression is selectively up-regulated by nicotine and has lower single channel conductance and calcium permeability (PubMed:17132685). In the striatum, also forms CHRNA4:CHRNA6:CHRNB2 complexes (By similarity). Also found in the stoichiometric form: (CHRNA4:CHRNB2)2:CHRNB3 (PubMed:16835356). Interacts with RIC3; which is required for proper folding and assembly (PubMed:16120769). Interacts with LYPD6 (PubMed:27344019)
Database

Entrez Gene: 1137 Human ; 11438 Mouse ; 25590 Rat

SwissProt: P43681 Human ; O70174 Mouse ; P09483 Rat

OMIM: 600513 Human

Research Field

-
Synonyms
ACH4; AChR; Acra4; BFNC; CHRNA4; EBN; EBN1; NACHR; NACRA4
Documentation
SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Nicotinic Acetylcholine Receptor alpha 4 Antibody (YA2616)
Cat. No.:
HY-P82871
Quantity:
MCE Japan Authorized Agent:

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Country or Region *

Email Address *

     

Phone Number *

 

Organization Name *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (252 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.