Copy Link Print Information

EXT1 Antibody (YA8961)

(Synonyms: Exostosin-1, Exostosin glycosyltransferase 1, Heparan sulfate co-polymerase subunit EXT1, Multiple exostoses protein 1, N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase, EXT1)
Customer Review

Based on 1 Customer Validation

EXT1 Antibody (YA8961) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EXT1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IF-Tissue, IP, ELISA

  • Reactivity :

    human, mouse, rat

  • Formulation:

    Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for EXT1 Antibody (YA8961)
  • EXT1 Antibody (YA8961) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IF-Tissue Info
IF-Tissue: Immunofluorescence-Tissue
IP Info
IP: Immunoprecipitation
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:100-1000 1:50-500 1:50-500 1-2μg per 100-500μg Total protein 1:30-3000

Product Details

Description

EXT1 Antibody (YA8961) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to EXT1.

  • Host Mouse
  • Species Reactivity
    human, mouse, rat
  • Calculated Molecular Weight Predicted band size: 86 kDa
Species Reactivity Database

OMIM: 608177

Immunogen

A synthesized peptide derived from human EXT1.

Sensitivity

Endogenous

Purification

Affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS(pH7.4) containing 0.1% gelatin and < 0.1% sodium azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at 2-8°C for 1 year, do not freeze.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for EXT1 Antibody (YA8961)
    Western blot analysis was performed on protein extracts (35 μg) from U87 (lane 2) using EXT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    EXT1 is a Glycosyltransferase forming with EXT2 the heterodimeric heparan sulfate polymerase which catalyzes the elongation of the heparan sulfate glycan backbone. Glycan backbone extension consists in the alternating transfer of (1->4)-beta-D-GlcA and (1->4)-alpha-D-GlcNAc residues from their respective UDP-sugar donors. Both EXT1 and EXT2 are required for the full activity of the polymerase since EXT1 bears the N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity within the complex while EXT2 carries the glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity. Heparan sulfate proteoglycans are ubiquitous components of the extracellular matrix and play an important role in tissue homeostasis and signaling[1][2][3][4][5][6].

  • Subcellular Localization

    Golgi apparatus membrane; Golgi apparatus, cis-Golgi network membrane; Endoplasmic reticulum membrane

  • Expression


    Tissue_Specificity: Widely expressed.

  • Isoforms & Post-Translational Modification

    EXT1 has an amino acid length of 746, molecular weight is 86255 Da.
    N-glycosylated

  • Subunit

    Part of the heparan sulfate polymerase, a dimeric complex composed of EXT1 and EXT2.

  • SwissProt ID

    Q16394

  • Gene ID
    2131 [NCBI]
  • Synonyms

    Exostosin-1, Exostosin glycosyltransferase 1, Heparan sulfate co-polymerase subunit EXT1, Multiple exostoses protein 1, N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase, EXT1

[1]. McCormick C, et al. The putative tumor suppressors EXT1 and EXT2 form a stable complex that accumulates in the Golgi apparatus and catalyzes the synthesis of heparan sulfate. Proc Natl Acad Sci U S A. 2000 Jan 18;97(2):668-73. [Content Brief]

[2]. Baietti MF, et al. Syndecan-syntenin-ALIX regulates the biogenesis of exosomes. Nat Cell Biol. 2012 Jun 3;14(7):677-85. [Content Brief]

[3]. Leisico F, et al. Structure of the human heparan sulfate polymerase complex EXT1-EXT2. Nat Commun. 2022 Nov 19;13(1):7110. [Content Brief]

[4]. Li H, et al. Structural basis for heparan sulfate co-polymerase action by the EXT1-2 complex. Nat Chem Biol. 2023 May;19(5):565-574. [Content Brief]

[5]. McCormick C, et al. The putative tumour suppressor EXT1 alters the expression of cell-surface heparan sulfate. Nat Genet. 1998 Jun;19(2):158-61. [Content Brief]

[6]. Cheung PK, et al. Etiological point mutations in the hereditary multiple exostoses gene EXT1: a functional analysis of heparan sulfate polymerase activity. Am J Hum Genet. 2001 Jul;69(1):55-66. [Content Brief]

View More

EXT1 Antibody (YA8961) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (35 μg) from U87 (lane 2) using EXT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • EXT1 Antibody (YA8961) Product Image
    1/16
  • Experimental Validation Results for EXT1 Antibody (YA8961)
  • EXT1 Antibody (YA8961) Product Image
  • Experimental Validation Results for EXT1 Antibody (YA8961)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (35 μg) from U87 (lane 2) using EXT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for EXT1 Antibody (YA8961)
  • EXT1 Antibody (YA8961) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (35 μg) from U87 (lane 2) using EXT1 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • EXT1 Antibody (YA8961) Product Image WB