LEF1 Antibody (YA5902)
(Synonyms: LEF1; Lymphoid enhancer-binding factor 1; LEF-1; T cell-specific transcription factor 1-alpha; TCF1-alpha)LEF1 Antibody (YA5902) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LEF1.
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Host:
Rabbit
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Isotype:
IgG
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Application:
WB, IHC-P, ICC/IF, IP, ELISA
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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WB
WB: Western Blot
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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IP
IP: Immunoprecipitation
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|---|---|---|---|---|---|
| Dilution Ratio | 1:1000-1:4000 | 1:2000-1:10000 | 1:200-1:1000 | 1:5000-1:20000 | 1:50-1:200 |
Product Details
LEF1 Antibody (YA5902) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to LEF1.
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Host Rabbit
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Observed Molecular WeightObserved band size: 45-60 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 44 kDa
Protein A
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
LEF1 transcription factor that binds DNA in a sequence-specific manner. Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression. Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling. May play a role in hair cell differentiation and follicle morphogenesis; Transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells; Lacks the CTNNB1 interaction domain and may therefore be an antagonist for Wnt signaling; Transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells[1][2][3].
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Subcellular Localization
Nucleus
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Expression
Tissue_specificity:Detected in the thymus. Not detected in normal colon, but highly expressed in colon cancer biopsy tissue and colon cancer cell lines. Expressed in various pancreatic tumors, and weakly expressed in normal pancreatic tissue. Isomers 1 and 5 were detected in various pancreatic cell lines. -
Isoforms & Post-Translational Modification
Q9UJU2 has 7 isomers: Q9UJU2-1: 44201 Da (predicted); Q9UJU2-2: 35672 Da (predicted); Q9UJU2-3: 31919 Da (predicted); Q9UJU2-4: 23390 Da (predicted); Q9UJU2-5: 41162 Da (predicted); Q9UJU2-6: 42676 Da (predicted); Q9UJU2-7: 34103 Da (predicted).
Phosphorylated at Thr-155 and/or Ser-166 by NLK. Phosphorylation by NLK at these sites represses LEF1-mediated transcriptional activation of target genes of the canonical Wnt signaling pathway -
Subunit
Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG (By similarity). Binds ALYREF/THOC4, MDFI and MDFIC. Interacts with NLK. Interacts with DAZAP2 (PubMed:19304756)
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SwissProt ID
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Synonyms
LEF1; Lymphoid enhancer-binding factor 1; LEF-1; T cell-specific transcription factor 1-alpha; TCF1-alpha
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