Copy Link Print Information

IFT88 Antibody (YA5910)

Customer Review

Based on 1 Customer Validation

IFT88 Antibody (YA5910) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IFT88.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG/Kappa

  • Application:

    WB, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for IFT88 Antibody (YA5910)
  • IFT88 Antibody (YA5910) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

IFT88 Antibody (YA5910) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IFT88.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 94 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 94 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG/Kappa

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IFT88 Antibody (YA5910)
    Western blot analysis of extracts from K562 (lane 2, 20ug), U251 (lane3, 20μg) using IFT88 Antibody (HY-P86218). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    IFT88 is a Positively regulates primary cilium biogenesis. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment[1].

  • Subcellular Localization

    Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole; Cell projection, cilium; Cytoplasm, cytoskeleton, cilium basal body; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm; Cell projection, cilium, flagellum; Cytoplasm, cytoskeleton

  • Expression


    Tissue_specificity:It is expressed in the heart, brain, liver, lungs, kidneys, skeletal muscle, and pancreas.

  • Isoforms & Post-Translational Modification

    Q13099 has 3 isomers: Q13099-2: 93192 Da (predicted); Q13099-1: 94270 Da (predicted); Q13099-3: 92381 Da (predicted).

  • Subunit

    Component of the IFT complex B, at least composed of IFT20, IFT22, IFT25, IFT27, IFT46, IFT52, TRAF3IP1/IFT54, IFT57, IFT74, IFT80, IFT81, and IFT88 (By similarity)

  • SwissProt ID

    Q13099

  • Gene ID
    8100 [NCBI]

IFT88 Antibody (YA5910) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2, 20ug), U251 (lane3, 20μg) using IFT88 Antibody (HY-P86218). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • IFT88 Antibody (YA5910) Product Image
    1/16
  • Experimental Validation Results for IFT88 Antibody (YA5910)
  • IFT88 Antibody (YA5910) Product Image
  • Experimental Validation Results for IFT88 Antibody (YA5910)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from K562 (lane 2, 20ug), U251 (lane3, 20μg) using IFT88 Antibody (HY-P86218). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for IFT88 Antibody (YA5910)
  • IFT88 Antibody (YA5910) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from K562 (lane 2, 20ug), U251 (lane3, 20μg) using IFT88 Antibody (HY-P86218). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • IFT88 Antibody (YA5910) Product Image WB