GNB2 Antibody (YA1613)

製品番号: HY-P81868: データシート SDS COA User Guide for Antibodies
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GNB2 Antibody (YA1613) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GNB2.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$84	在庫あり	Estimated Time of Arrival: December 31
50 μL	$222	在庫あり	Estimated Time of Arrival: December 31
100 μL	$360	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明
参考文献

製品説明

GNB2 Antibody (YA1613) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GNB2.

Host

Rabbit

Clonality

Recombinant, Monoclonal

分子量

Predicted band size: 37 kDa;

Observed band size: 32 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P62879

Gene ID

2783 [NCBI]

Immunogen

A synthetic peptide of human GNB2

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	純度	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis was performed on extracts from Hela (lane 1, 15 μg), SH-SY5Y (lane 2, 15 μg), Jurkat (lane 3, 15 μg), C6 (lane 4, 15 μg), Mouse brain (lane 5, 15 μg), and Rat brain (lane 6, 15 μg) using GNB2 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:20000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

Background

Function：Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction

Subcellular Localization：Cytoplasm, perinuclear region; Cell membrane

Expression：
Tissue_specificity:Expressed in all cardiac subcompartments and in the brain, with highest levels in the atrioventricular node and brain

Isoforms & Post-Translational Modification：P62879 has 2 isomers: P62879-1: 37331 Da (predicted); P62879-2: 25931 Da (predicted).

Subunit：G proteins are composed of 3 units, alpha, beta and gamma. In this context, interacts with GNAI2 and GNG2 (PubMed:28219978). Interacts with ARHGEF18 and RASD2. Interacts with ATXN10. Interacts with SCN8A (PubMed:26900580)

Database

Entrez Gene: 2783 Human ; 14693 Mouse ; 81667 Rat

SwissProt: P62879 Human ; P62880 Mouse ; P54313 Rat

OMIM: 619503 Human

Research Field

Signal Transduction

Synonyms

Gnb2; Gnb2l1; RACK1; Transducin beta chain 2

ドキュメンテーション

Select Batch:

データシート (258 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/a549-cell.html [Content Brief]