Cat. No.: HY-P80565

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Bcl-2 Antibody (YA824)

(Synonyms: BCL2; Apoptosis regulator Bcl-2)

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Bcl-2 Antibody (YA824) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Bcl-2.

For research use only. We do not sell to patients.

Host:
Mouse
Isotype:
IgG
Application:
WB
Reactivity :
Human, Rat, Mouse

Formulation:
Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Conjugation:
Non-conjugated

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Quantity

Amount: USD 0.00

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Applications
Product Details
Product Properties
- Appearance
- Formulation
- Concentration
- Storage & Stability
- Shipping
Verification Images
Background
Documentation
References
Help & FAQs
Classifications
Bioz

Applications

Application	WB WB: Western Blot
Dilution Ratio	1:500-1:1000

Product Details

Description

Bcl-2 Antibody (YA824) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Bcl-2.

Host Mouse
Clonality Monoclonal
Species Reactivity
Human, Rat, Mouse
Observed Molecular Weight

Observed band size: 26 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Calculated Molecular Weight Predicted band size: 26 kDa

Species Reactivity Database

Entrez Gene: 596 Human ; 12043 Mouse ; 24224 Rat

SwissProt: P10415 Human ; P10417 Mouse ; P49950 Rat

OMIM: 151430 Human

Immunogen

Synthetic peptide corresponding to Human Bcl-2 conjugated to KLH.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102132

Product Properties

Appearance

Solution
Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.
Concentration

Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Shipping

Shipping with blue ice.

Verification Images

Western blot analysis of extracts from HeLa (lane 1(20μg)) 、SH-SY5Y (lane 2(20μg)) 、HepG2 (lane 3(20μg)) 、HEK293 (lane 4(20μg)) 、Jurkat (lane 5(20μg)) 、HL-60 (lane 6(20μg)) 、U937 (lane 7(20μg)) and MCF7 (lane 8(20μg)) using Bcl-2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P80565, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg), 3T3(lane 2, 20 μg) using AP2M1 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.
Western blot analysis of extracts from HeLa (lane 1(20μg)) 、SH-SY5Y (lane 2(20μg)) 、HepG2 (lane 3(20μg)) 、HEK293 (lane 4(20μg)) 、Jurkat (lane 5(20μg)) 、HL-60 (lane 6(20μg)) 、U937 (lane 7(20μg)) and MCF7 (lane 8(20μg)) using Bcl-2 Antibody (HY-P80565) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
Western blot analysis of extracts from Jurkat(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HL-60(lane 4(20ug) using Bcl-2 Antibody (HY-P80565) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function

Bcl-2 (B-cell lymphoma 2) is a central anti-apoptotic regulator that preserves mitochondrial integrity by preventing mitochondrial outer membrane permeabilization (MOMP)， a critical checkpoint controlling cytochrome c release and caspase activation during intrinsic apoptosis^[1]^[2]. Mechanistically, Bcl-2 functions through direct interactions with pro-apoptotic BCL-2 family proteins, including Bax and Bak, thereby suppressing their oligomerization and limiting mitochondrial membrane disruption^[3]^[4]. The protein also integrates signals transmitted by BH3-only proteins, which regulate the balance between cell survival and programmed cell death under physiological and pathological stress conditions^[3]^[5]. Dysregulated Bcl-2 expression contributes to apoptosis resistance in multiple malignancies and supports tumor cell survival by maintaining mitochondrial homeostasis despite cellular stress^[6]^[7]. In disease models, the anti-apoptotic activity of Bcl-2 is closely linked to mitochondrial apoptosis pathways that represent major therapeutic targets in hematologic and solid cancers^[6]^[8]. Compared with related anti-apoptotic isoforms such as Mcl-1 and Bcl-B, Bcl-2 displays broader interactions with both Bax and Bak, whereas Mcl-1 and Bcl-B show preferential regulation of Bak- and Bax-dependent apoptotic pathways, respectively^[4]. For experimental applications, BH3-mimetic inhibitors disrupt the interaction between Bcl-2 and pro-apoptotic partners, thereby restoring mitochondrial apoptosis and providing a widely used strategy for mechanistic studies and targeted cancer research^[7]^[8].
Subcellular Localization

Mitochondrion outer membrane; Single-pass membrane protein; Nucleus membrane; Single-pass membrane protein; Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasm
Expression

Tissue_specificity:Expression in multiple organizations
Isoforms & Post-Translational Modification

P10415 has 2 isomers: P10415-1: 26266 Da (predicted); P10415-2: 22337 Da (predicted).
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity (PubMed:11368354). Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle (PubMed:11368354). In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases (PubMed:11368354). Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, which stimulates starvation-induced autophagy (PubMed:10567572, PubMed:18570871). Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity);Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity;Monoubiquitinated by PRKN, leading to an increase in its stability (PubMed:20889974). Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome (PubMed:23431138). Ubiquitinated by XIAP, leading to its degradation by the proteasome (PubMed:29020630)
Subunit

Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (PubMed:25609812, PubMed:8183370).
SwissProt ID

P10415
Gene ID

596 [NCBI]
Synonyms

BCL2; Apoptosis regulator Bcl-2
Research Field

Cell Biology