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Bax Antibody (YA591)

(Synonyms: BCL2L4, BAX, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4)
10 Cited Publications
Customer Review

Based on 10 publication(s) in Google Scholar

Bax Antibody (YA591) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Bax.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP, FC

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB FC ICC
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Bax Antibody (YA591) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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10 Publications Citing Use of MCE Bax Antibody (YA591) (7)
WB
  • WB
  • WB
  • WB
  • WB
  • WB
  • WB
  • WB

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:5000 1:100 1:50-1:100 Use at an assay dependent concentration.

Product Details

Description

Bax Antibody (YA591) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Bax.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human, Mouse, Rat Predicted Reactivity: Chicken,Goat,Pig
    Info
    Note: The predicted reactivity is for reference only and should not be considered a guarantee of product performance.
  • Observed Molecular Weight
    Observed band size: 21 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 21 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human Bax.AA range:1-50.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102269

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Bax Antibody (YA591)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P80028, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bax Antibody (YA591)
    Western blot analysis of extracts from Hela (lane 2(20μg) , C6 (lane 3(20μg) , HEK293 (lane 4(20μg),using Bax Antibody (HY-P80028). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bax Antibody (YA591)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody (HY-P80028) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bax Antibody (YA591)
    Flow cytometric analysis of 1X10^6 Hela cells labeling Bax Antibody(HY-P80028, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/mL dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Bax Antibody (YA591)
    Immunocytochemistry analysis of SHSY5Y cells labeling Bax with Bax Antibody (HY-P80028) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Bax Antibody (HY-P80028) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Bax Antibody (YA591)
    Immunocytochemistry analysis of HepG2 cells labeling Bax with Bax Antibody (HY-P80028) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Bax Antibody (HY-P80028)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Plays a role in the mitochondrial apoptotic process (PubMed:10772918, PubMed:11060313, PubMed:16113678, PubMed:16199525, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:36361894, PubMed:8358790, PubMed:8521816). Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed:21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis (PubMed:10772918, PubMed:11060313, PubMed:16113678, PubMed:16199525, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:8358790, PubMed:8521816). Promotes activation of CASP3, and thereby apoptosis (PubMed:10772918, PubMed:11060313, PubMed:16113678, PubMed:16199525, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:8358790, PubMed:8521816)

  • Subcellular Localization

    Mitochondrion outer membrane; Single-pass membrane protein; Cytoplasm; Nucleus; Cytoplasm; Cytoplasm; Cytoplasm

  • Expression


    Tissue_specificity:This gene is expressed in a variety of tissues. The Psi isoform is present in gliomas. The α isoform is expressed in the spleen, breast, ovary, testis, colon, and brain, and at low levels in the skin and lungs. The Sigma isoform is expressed in the spleen, breast, ovary, testis, lung, colon, and brain, and at low levels in the skin. Both the α and Sigma isoforms are expressed in promyelocytic leukemia, histiocytic lymphoma, Burkitt lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovarian adenocarcinoma, prostate cancer, prostate adenocarcinoma, lung cancer, epidermoid carcinoma, small cell lung cancer, and colon adenocarcinoma cell lines.

  • Subunit

    Homodimer. Forms higher oligomers under stress conditions. Forms heterooligomers with BAK (PubMed:29531808). Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1 (PubMed:25609812). Interacts with SH3GLB1. Interacts with humanin; forms fibers with humanin which results in BAX conformational changes and sequestering of BAX into the fibers, preventing BAX activation (PubMed:12732850, PubMed:31690630). Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2. Interacts with RTL10/BOP. Interacts (via a C-terminal 33 residues) with NOL3 (via CARD domain); inhibits BAX activation and translocation and consequently cytochrome c release from mitochondria. Interacts with GIMAP3/IAN4 and GIMAP5/IAN5; this interaction is increased, when cells initiate apoptosis upon IL2 withdrawal (PubMed:16509771). Interacts with IRF3; the interaction is direct, increases upon Sendai virus infection and mediates the formation of the apoptosis complex TOMM70:HSP90AA1:IRF3:BAX (PubMed:25609812). Interacts with MOAP1, facilitating BAX-dependent mitochondrial outer membrane permeabilization and apoptosis (PubMed:11060313, PubMed:16199525). Interacts with BCL2L10/BCL-B (PubMed:23235460). Interacts with non-acetylated XRCC6/Ku70; this interaction leads to BAX sequestration in the cytosol, away from the mitochondria, preventing BAX-mediated apoptosis (PubMed:15023334)

  • SwissProt ID

    Q07812

  • Gene ID
    581 [NCBI]
  • Synonyms

    BCL2L4, BAX, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4

  • Research Field

    Cell Biology

Bax Antibody (YA591) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P80028, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) , C6 (lane 3(20μg) , HEK293 (lane 4(20μg),using Bax Antibody (HY-P80028). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody (HY-P80028) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling Bax Antibody(HY-P80028, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/mL dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of SHSY5Y cells labeling Bax with Bax Antibody (HY-P80028) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Bax Antibody (HY-P80028) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Bax with Bax Antibody (HY-P80028) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Bax Antibody (HY-P80028)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    Bax Antibody (YA591) purchased from MedChemExpress. Usage Cited in: J Photochem Photobiol B. 2026 Jan:274:113350. [Abstract]

    Customer Validation
     Alterations in apoptosis-associated proteins (caspase-3, caspase-8, Bax, Bcl-2) in HOS cells assessed by western blot.

  • 1/16

    Bax Antibody (YA591) purchased from MedChemExpress. Usage Cited in: J Med Chem. 2025 Aug 14;68(15):16138-16171. [Abstract]

    Customer Validation
     MDA-MB-231 cells were incubated with either 59 or DMSO for a period of 48h. After the treatment was completed, the cells were collected and washed three times with 1 mL of cold PBS. Subsequently, they were lysed in 50 μL of ice-cold RIPA buffer for 10 min. The cell lysates were then subjected to centrifugation at 12,000 rpm for 15 min at 4 °C. The protein concentration within the lysates was determined using the BCA assay. After lysis, the proteins were separated by SDS-PAGE and then transferred onto PVDF membranes. These membranes were then individually treated with a series of antibodies, including ROR1 antibody , Phosphorylated ROR1 antibody, cleaved PARP antibody (HY-P80448), Bax antibody (HY-P80028), BCL-2 antibody (HY-P80566) and GAPDH antibody. Afterward, the membranes were probed with a secondary antibody conjugated with horseradish peroxidase (obtained from Cell Signaling Technology). Finally, the blots were visualized using an enhanced chemiluminescence system.

  • 1/16

    Bax Antibody (YA591) purchased from MedChemExpress. Usage Cited in: Life Sci. 2025 Sep 1:376:123746. [Abstract]

    Customer Validation
     WB detection of Bcl-2, Bax, c-caspase3, and caspase3 expression.

  • 1/16

    Bax Antibody (YA591) purchased from MedChemExpress. Usage Cited in: Colloids Surf A Physicochem Eng Asp. 2025 Aug 20.

    Customer Validation
     Western blot analysis of the expressions of Bcl-2 and Bax, in C5WN1 cells after treated with Control, DOX@MPDA-GR, and DQ@MPDA-GR.

  • 1/16

    Bax Antibody (YA591) purchased from MedChemExpress. Usage Cited in: Int Dent J. 2025 Oct 8;75(6):103897. [Abstract]

    Customer Validation
     OSCC cells were treated with different concentrations of glucose for 48 hours, and Western blots were performed to detect the expression of N-cadherin, E-cadherin, vimentin, Bax, and Bcl-2. b-actin was used as the internal loading control.

  • 1/16

    Bax Antibody (YA591) purchased from MedChemExpress. Usage Cited in: Int Dent J. 2025 Oct 8;75(6):103897. [Abstract]

    Customer Validation
     Expression of O-GlcNAcylation, OGT, and representative EMT and cell apoptosis markers of OSCC cells exposed to a medium containing 25 mM glucose and/or OGT knockdown were examined using Western blot analysis.

  • 1/16

    Bax Antibody (YA591) purchased from MedChemExpress. Usage Cited in: Transl Cancer Res. 2024 Jun 30;13(6):2596-2604. [Abstract]

    Customer Validation
     The expression of PCNA, Bax, vimentin, and E-cadherin was analyzed by western blotting in RKO cells. β-actin was used as a control and the control group was homogenized.

  • Bax Antibody (YA591) Product Image
    1/16
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Bax Antibody (YA591) Product Image
  • Experimental Validation Results for Bax Antibody (YA591)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P80028, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bax Antibody (YA591)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Hela (lane 2(20μg) , C6 (lane 3(20μg) , HEK293 (lane 4(20μg),using Bax Antibody (HY-P80028). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bax Antibody (YA591)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody (HY-P80028) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bax Antibody (YA591)
    1/1
    MedchemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling Bax Antibody(HY-P80028, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/mL dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for Bax Antibody (YA591)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of SHSY5Y cells labeling Bax with Bax Antibody (HY-P80028) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Bax Antibody (HY-P80028) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Bax Antibody (YA591)
    1/1
    MedchemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling Bax with Bax Antibody (HY-P80028) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Bax Antibody (HY-P80028)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)
  • Experimental Validation Results for Bax Antibody (YA591)

  • Bax Antibody (YA591) purchased from MedChemExpress. Usage Cited in: J Photochem Photobiol B. 2026 Jan:274:113350. [Abstract]

    Alterations in apoptosis-associated proteins (caspase-3, caspase-8, Bax, Bcl-2) in HOS cells assessed by western blot.

  • Bax Antibody (YA591) purchased from MedChemExpress. Usage Cited in: J Med Chem. 2025 Aug 14;68(15):16138-16171. [Abstract]

    MDA-MB-231 cells were incubated with either 59 or DMSO for a period of 48h. After the treatment was completed, the cells were collected and washed three times with 1 mL of cold PBS. Subsequently, they were lysed in 50 μL of ice-cold RIPA buffer for 10 min. The cell lysates were then subjected to centrifugation at 12,000 rpm for 15 min at 4 °C. The protein concentration within the lysates was determined using the BCA assay. After lysis, the proteins were separated by SDS-PAGE and then transferred onto PVDF membranes. These membranes were then individually treated with a series of antibodies, including ROR1 antibody , Phosphorylated ROR1 antibody, cleaved PARP antibody (HY-P80448), Bax antibody (HY-P80028), BCL-2 antibody (HY-P80566) and GAPDH antibody. Afterward, the membranes were probed with a secondary antibody conjugated with horseradish peroxidase (obtained from Cell Signaling Technology). Finally, the blots were visualized using an enhanced chemiluminescence system.

  • Bax Antibody (YA591) purchased from MedChemExpress. Usage Cited in: Life Sci. 2025 Sep 1:376:123746. [Abstract]

    WB detection of Bcl-2, Bax, c-caspase3, and caspase3 expression.

  • Bax Antibody (YA591) purchased from MedChemExpress. Usage Cited in: Colloids Surf A Physicochem Eng Asp. 2025 Aug 20.

    Western blot analysis of the expressions of Bcl-2 and Bax, in C5WN1 cells after treated with Control, DOX@MPDA-GR, and DQ@MPDA-GR.

  • Bax Antibody (YA591) purchased from MedChemExpress. Usage Cited in: Int Dent J. 2025 Oct 8;75(6):103897. [Abstract]

    OSCC cells were treated with different concentrations of glucose for 48 hours, and Western blots were performed to detect the expression of N-cadherin, E-cadherin, vimentin, Bax, and Bcl-2. b-actin was used as the internal loading control.

  • Bax Antibody (YA591) purchased from MedChemExpress. Usage Cited in: Int Dent J. 2025 Oct 8;75(6):103897. [Abstract]

    Expression of O-GlcNAcylation, OGT, and representative EMT and cell apoptosis markers of OSCC cells exposed to a medium containing 25 mM glucose and/or OGT knockdown were examined using Western blot analysis.

  • Bax Antibody (YA591) purchased from MedChemExpress. Usage Cited in: Transl Cancer Res. 2024 Jun 30;13(6):2596-2604. [Abstract]

    The expression of PCNA, Bax, vimentin, and E-cadherin was analyzed by western blotting in RKO cells. β-actin was used as a control and the control group was homogenized.

  • Bax Antibody (YA591) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P80028, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Bax Antibody (YA591) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Hela (lane 2(20μg) , C6 (lane 3(20μg) , HEK293 (lane 4(20μg),using Bax Antibody (HY-P80028). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody and Loading control antibody (Beta Actin, HY-P80438, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

  • Bax Antibody (YA591) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody (HY-P80028) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Bax Antibody (YA591) Product Image
    MedchemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling Bax Antibody(HY-P80028, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1μg/mL dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Bax Antibody (YA591) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of SHSY5Y cells labeling Bax with Bax Antibody (HY-P80028) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Bax Antibody (HY-P80028) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Bax Antibody (YA591) Product Image
    MedchemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling Bax with Bax Antibody (HY-P80028) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Bax Antibody (HY-P80028)at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 594-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8003,Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Bax Antibody (YA591) Product Image WB
  • Bax Antibody (YA591) Product Image WB
  • Bax Antibody (YA591) Product Image WB
  • Bax Antibody (YA591) Product Image FC
  • Bax Antibody (YA591) Product Image ICC
  • Bax Antibody (YA591) Product Image ICC