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Bcl-2 Antibody (YA6113)

(Synonyms: BCL2; Apoptosis regulator Bcl-2)
Customer Review

Based on 1 Customer Validation

Bcl-2 Antibody (YA6113) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Bcl-2.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
ALL WB IHC-P
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Bcl-2 Antibody (YA6113) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:2000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

Bcl-2 Antibody (YA6113) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Bcl-2.

  • Host Rabbit
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 25 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 26 kDa
Purification

Protein A

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3719152

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    Western blot analysis of extracts from Hela (lane2, 20μg) using Bcl-2 Antibody (HY-P86421). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、SH-SY5Y (lane 2(20μg)) 、HepG2 (lane 3(20μg)) 、HEK293 (lane 4(20μg)) 、Jurkat (lane 5(20μg)) 、HL-60 (lane 6(20μg)) 、U937 (lane 7(20μg)) and MCF7 (lane 8(20μg)) using Bcl-2 Antibody (HY-P86421) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、SH-SY5Y (lane 2(20μg)) 、HepG2 (lane 3(20μg)) 、HEK293 (lane 4(20μg)) 、Jurkat (lane 5(20μg)) 、HL-60 (lane 6(20μg)) 、U937 (lane 7(20μg)) and MCF7 (lane 8(20μg)) using Bcl-2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P86421, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Bcl-2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/400) , competitor's antibody (1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    Immunohistochemical analysis of paraffin-embedded human follicular lymphoma tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell Lymphoma tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    Immunohistochemical analysis of paraffin-embedded human hodgkin's lymphoma tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    Immunohistochemical analysis of paraffin-embedded human spleen tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

Background

  • Function

    Bcl-2 (B-cell lymphoma 2) is a central anti-apoptotic regulator that preserves mitochondrial integrity by preventing mitochondrial outer membrane permeabilization (MOMP), a critical checkpoint controlling cytochrome c release and caspase activation during intrinsic apoptosis[1][2]. Mechanistically, Bcl-2 functions through direct interactions with pro-apoptotic BCL-2 family proteins, including Bax and Bak, thereby suppressing their oligomerization and limiting mitochondrial membrane disruption[3][4]. The protein also integrates signals transmitted by BH3-only proteins, which regulate the balance between cell survival and programmed cell death under physiological and pathological stress conditions[3][5]. Dysregulated Bcl-2 expression contributes to apoptosis resistance in multiple malignancies and supports tumor cell survival by maintaining mitochondrial homeostasis despite cellular stress[6][7]. In disease models, the anti-apoptotic activity of Bcl-2 is closely linked to mitochondrial apoptosis pathways that represent major therapeutic targets in hematologic and solid cancers[6][8]. Compared with related anti-apoptotic isoforms such as Mcl-1 and Bcl-B, Bcl-2 displays broader interactions with both Bax and Bak, whereas Mcl-1 and Bcl-B show preferential regulation of Bak- and Bax-dependent apoptotic pathways, respectively[4]. For experimental applications, BH3-mimetic inhibitors disrupt the interaction between Bcl-2 and pro-apoptotic partners, thereby restoring mitochondrial apoptosis and providing a widely used strategy for mechanistic studies and targeted cancer research[7][8].

  • Subcellular Localization

    Mitochondrion outer membrane; Single-pass membrane protein; Nucleus membrane; Single-pass membrane protein; Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasm

  • Expression


    Tissue_specificity:Expression in multiple organizations

  • Isoforms & Post-Translational Modification

    P10415 has 2 isomers: P10415-1: 26266 Da (predicted); P10415-2: 22337 Da (predicted).
    Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity (PubMed:11368354). Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle (PubMed:11368354). In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases (PubMed:11368354). Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, which stimulates starvation-induced autophagy (PubMed:10567572, PubMed:18570871). Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity);Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity;Monoubiquitinated by PRKN, leading to an increase in its stability (PubMed:20889974). Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome (PubMed:23431138). Ubiquitinated by XIAP, leading to its degradation by the proteasome (PubMed:29020630)

  • Subunit

    Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (PubMed:25609812, PubMed:8183370).

  • SwissProt ID

    P10415

  • Gene ID
    596 [NCBI]
  • Synonyms

    BCL2; Apoptosis regulator Bcl-2

References

[1]. Harris MH, et al. The role of the Bcl-2 family in the regulation of outer mitochondrial membrane permeability. Cell Death Differ. 2000;7:1182-1191.

[2]. Breckenridge DG, et al. Regulation of mitochondrial membrane permeabilization by BCL-2 family proteins and caspases. Curr Opin Cell Biol. 2004;16(6):647-652.

[3]. Kale J, et al. BCL-2 family proteins: changing partners in the dance towards death. Cell Death Differ. 2018;25:65-80.

[4]. Zhai D, et al. Differential regulation of Bax and Bak by anti-apoptotic Bcl-2 family proteins Bcl-B and Mcl-1. J Biol Chem. 2008 Apr 11;283(15):9580-6. [Content Brief]

[5]. Lomonosova E, et al. BH3-only proteins in apoptosis and beyond: an overview. Oncogene. 2008 Dec;27 Suppl 1(Suppl 1):S2-19. [Content Brief]

[6]. Danial NN. BCL-2 family proteins: critical checkpoints of apoptotic cell death. Clin Cancer Res. 2007;13(24):7254-7263.

[7]. Akl H, et al. A dual role for the anti-apoptotic Bcl-2 protein in cancer: Mitochondria versus endoplasmic reticulum. Biochim Biophys Acta. 2014;1843(10):2240-2252.

[8]. Kang MH, et al. Bcl-2 inhibitors: targeting mitochondrial apoptotic pathways in cancer therapy. Clin Cancer Res. 2009 Feb 15;15(4):1126-32. [Content Brief]

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Bcl-2 Antibody (YA6113) Related Classifications

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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg) using Bcl-2 Antibody (HY-P86421). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、SH-SY5Y (lane 2(20μg)) 、HepG2 (lane 3(20μg)) 、HEK293 (lane 4(20μg)) 、Jurkat (lane 5(20μg)) 、HL-60 (lane 6(20μg)) 、U937 (lane 7(20μg)) and MCF7 (lane 8(20μg)) using Bcl-2 Antibody (HY-P86421) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、SH-SY5Y (lane 2(20μg)) 、HepG2 (lane 3(20μg)) 、HEK293 (lane 4(20μg)) 、Jurkat (lane 5(20μg)) 、HL-60 (lane 6(20μg)) 、U937 (lane 7(20μg)) and MCF7 (lane 8(20μg)) using Bcl-2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P86421, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Bcl-2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/400) , competitor's antibody (1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human follicular lymphoma tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell Lymphoma tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human hodgkin's lymphoma tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymphoma tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Bcl-2 Antibody (YA6113) Product Image
    1/16
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Bcl-2 Antibody (YA6113) Product Image
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2, 20μg) using Bcl-2 Antibody (HY-P86421). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、SH-SY5Y (lane 2(20μg)) 、HepG2 (lane 3(20μg)) 、HEK293 (lane 4(20μg)) 、Jurkat (lane 5(20μg)) 、HL-60 (lane 6(20μg)) 、U937 (lane 7(20μg)) and MCF7 (lane 8(20μg)) using Bcl-2 Antibody (HY-P86421) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、SH-SY5Y (lane 2(20μg)) 、HepG2 (lane 3(20μg)) 、HEK293 (lane 4(20μg)) 、Jurkat (lane 5(20μg)) 、HL-60 (lane 6(20μg)) 、U937 (lane 7(20μg)) and MCF7 (lane 8(20μg)) using Bcl-2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P86421, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Bcl-2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/400) , competitor's antibody (1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human follicular lymphoma tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell Lymphoma tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human hodgkin's lymphoma tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human lymphoma tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
    1/1
    MedChemExpress Validation
     Immunohistochemical analysis of paraffin-embedded human spleen tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Experimental Validation Results for Bcl-2 Antibody (YA6113)
  • Bcl-2 Antibody (YA6113) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2, 20μg) using Bcl-2 Antibody (HY-P86421). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/5000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HY-P8001,1/10,000) was used for 1 hour at room temperature.

  • Bcl-2 Antibody (YA6113) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、SH-SY5Y (lane 2(20μg)) 、HepG2 (lane 3(20μg)) 、HEK293 (lane 4(20μg)) 、Jurkat (lane 5(20μg)) 、HL-60 (lane 6(20μg)) 、U937 (lane 7(20μg)) and MCF7 (lane 8(20μg)) using Bcl-2 Antibody (HY-P86421) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Bcl-2 Antibody (YA6113) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、SH-SY5Y (lane 2(20μg)) 、HepG2 (lane 3(20μg)) 、HEK293 (lane 4(20μg)) 、Jurkat (lane 5(20μg)) 、HL-60 (lane 6(20μg)) 、U937 (lane 7(20μg)) and MCF7 (lane 8(20μg)) using Bcl-2 Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P86421, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Bcl-2 Antibody (YA6113) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using Bcl-2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/400) , competitor's antibody (1/400) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Bcl-2 Antibody (YA6113) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Bcl-2 Antibody (YA6113) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Bcl-2 Antibody (YA6113) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human follicular lymphoma tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Bcl-2 Antibody (YA6113) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell Lymphoma tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Bcl-2 Antibody (YA6113) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human hodgkin's lymphoma tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Bcl-2 Antibody (YA6113) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Bcl-2 Antibody (YA6113) Product Image
    MedChemExpress Validation

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using Bcl-2 Antibody (YA6113). The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P86421, 1/300) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Bcl-2 Antibody (YA6113) Product Image WB
  • Bcl-2 Antibody (YA6113) Product Image WB
  • Bcl-2 Antibody (YA6113) Product Image WB
  • Bcl-2 Antibody (YA6113) Product Image IHC-P
  • Bcl-2 Antibody (YA6113) Product Image IHC-P
  • Bcl-2 Antibody (YA6113) Product Image IHC-P
  • Bcl-2 Antibody (YA6113) Product Image IHC-P
  • Bcl-2 Antibody (YA6113) Product Image IHC-P
  • Bcl-2 Antibody (YA6113) Product Image IHC-P
  • Bcl-2 Antibody (YA6113) Product Image IHC-P
  • Bcl-2 Antibody (YA6113) Product Image IHC-P