Bcl-2 Antibody (YA590)

Cat. No.: HY-P80566: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

Bcl-2 Antibody (YA590) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Bcl-2.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

or Bulk Inquiry

* Please select Quantity before adding items.

Bcl-2 Antibody (YA590) Featured Recommendations:

10 Publications Citing Use of MCE Bcl-2 Antibody (YA590)

: Bcl-2 Antibody (YA590) purchased from MCE. Usage Cited in: J Med Chem. 2025 Aug 14;68(15):16138-16171. [Abstract]; MDA-MB-231 cells were incubated with either 59 or DMSO for a period of 48h. After the treatment was completed, the cells were collected and washed three times with 1 mL of cold PBS. Subsequently, they were lysed in 50 μL of ice-cold RIPA buffer for 10 min. The cell lysates were then subjected to centrifugation at 12,000 rpm for 15 min at 4 °C. The protein concentration within the lysates was determined using the BCA assay. After lysis, the proteins were separated by SDS-PAGE and then transferred onto PVDF membranes. These membranes were then individually treated with a series of antibodies, including ROR1 antibody , Phosphorylated ROR1 antibody, cleaved PARP antibody (HY-P80448), Bax antibody (HY-P80028), BCL-2 antibody (HY-P80566) and GAPDH antibody. Afterward, the membranes were probed with a secondary antibody conjugated with horseradish peroxidase (obtained from Cell Signaling Technology). Finally, the blots were visualized using an enhanced chemiluminescence system.

: Bcl-2 Antibody (YA590) purchased from MCE. Usage Cited in: Colloids Surf A Physicochem Eng Asp. 2025 Aug 20.; Western blot analysis of the expressions of Bcl-2 and Bax, in C5WN1 cells after treated with Control, DOX@MPDA-GR, and DQ@MPDA-GR.

: Bcl-2 Antibody (YA590) purchased from MCE. Usage Cited in: Int Dent J. 2025 Oct 8;75(6):103897. [Abstract]; OSCC cells were treated with different concentrations of glucose for 48 hours, and Western blots were performed to detect the expression of N-cadherin, E-cadherin, vimentin, Bax, and Bcl-2.

: Bcl-2 Antibody (YA590) purchased from MCE. Usage Cited in: Rep Phys Sci. 2024 July 01.; Western blotting analysis of the apoptosis-related protein expression (cleaved caspase-3 and Bcl-2) in tumor tissues after different treatments (n = 3).

: Bcl-2 Antibody (YA590) purchased from MCE. Usage Cited in: Respir Res. 2024 Nov 7;25(1):399. [Abstract]; The relative protein levels of BCL2, BAX, and β-catenin were determined by western blot analysis and quantified.

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Documentation

Description

Bcl-2 Antibody (YA590) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Bcl-2.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 26 kDa;

Observed band size: 26 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

P10415

Gene ID

596 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Bcl-2 aa1-211.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

ALL WB IHC-P

Western blot analysis of extracts from NIH3T3 (lane 2(20μg), K562 (lane 3(20μg) and Hela(lane 4(20μg) using Bcl2 (HY-P80566) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from HeLa (lane 1(20μg)) 、SH-SY5Y (lane 2(20μg)) 、HepG2 (lane 3(20μg)) 、HEK293 (lane 4(20μg)) 、Jurkat (lane 5(20μg)) 、HL-60 (lane 6(20μg)) 、U937 (lane 7(20μg)) and MCF7 (lane 8(20μg)) using Bcl-2 Antibody (HY-P80566) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
Western blot analysis was performed on protein extracts (30 μg) from HT-1080 (lane 2), SH-SY5Y (lane 3), HeLa (lane 4), A549 (lane 5), and Caco-2 (lane 6) using Bcl-2 antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot^® Turbo^™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. Thhe primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:2500) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit IgG (H&L) secondary antibody (HY-P8001) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Bcl2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using Bcl2 Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:20889974, PubMed:21358617). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785)

Subcellular Localization：Mitochondrion outer membrane; Single-pass membrane protein; Nucleus membrane; Single-pass membrane protein; Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasm

Expression：
Tissue_specificity:Expression in multiple organizations

Isoforms & Post-Translational Modification：P10415 has 2 isomers: P10415-1: 26266 Da (predicted); P10415-2: 22337 Da (predicted).
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity (PubMed:11368354). Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle (PubMed:11368354). In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases (PubMed:11368354). Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, which stimulates starvation-induced autophagy (PubMed:10567572, PubMed:18570871). Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity);Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity;Monoubiquitinated by PRKN, leading to an increase in its stability (PubMed:20889974). Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome (PubMed:23431138). Ubiquitinated by XIAP, leading to its degradation by the proteasome (PubMed:29020630)

Subunit：Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (PubMed:25609812, PubMed:8183370).

RRID

AB_3102133

Database

Entrez Gene: 596 Human ; 12043 Mouse ;

SwissProt: P10415 Human ; P10417 Mouse ;

OMIM: 151430 Human

Research Field

Cell Biology

Synonyms

BCL2; Apoptosis regulator Bcl-2

Documentation

Select Batch:

Data Sheet (232 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Bcl-2 Antibody (YA590) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Your Recently Viewed Products:

Product Name

Requested Quantity *

Applicant Name *

Salutation

Email Address *

Phone Number *

Department

Organization Name *

City

State

Country or Region *

Remarks

Product Name

Lot #

Applicant Name *

Country or Region *

Email Address *

Remarks

Name
Email *

	Sorry, but the email address you supplied was invalid.

Bcl-2 Antibody (YA590)

Bcl-2 Antibody (YA590) Featured Recommendations:

10 Publications Citing Use of MCE Bcl-2 Antibody (YA590)

Product Detail

Verification Image

Background

Documentation

Bcl-2 Antibody (YA590) Related Classifications

Your Recently Viewed Products:

Request for HNMR Report

SAFETY DATA SHEET (SDS)

Request for HNMR Report