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Bax Antibody (YA825)

(Synonyms: Apoptosis regulator BAX; BAX; Bcl-2-like protein 4; BCL2-associated X protein; Bcl2-L-4; BCL2L4)
3 Cited Publications
Customer Review

Based on 3 publication(s) in Google Scholar

Bax Antibody (YA825) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Bax.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2b

  • Application:

    WB, IHC-P

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
ALL WB IHC IHC-P
  • Experimental Validation Results for Bax Antibody (YA825)
  • Experimental Validation Results for Bax Antibody (YA825)
  • Experimental Validation Results for Bax Antibody (YA825)
  • Experimental Validation Results for Bax Antibody (YA825)
  • Experimental Validation Results for Bax Antibody (YA825)
  • Experimental Validation Results for Bax Antibody (YA825)
  • Bax Antibody (YA825) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

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Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
Dilution Ratio 1:500-1:1000 1:50-1:100

Product Details

Description

Bax Antibody (YA825) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to Bax.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 21 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 21 kDa
Species Reactivity Database
Immunogen

Synthetic peptide corresponding to Human BAX.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2b

RRID

AB_3102268

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Bax Antibody (YA825)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P80027, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bax Antibody (YA825)
    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody (HY-P80027) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bax Antibody (YA825)
    Western blot analysis of extracts from Hela (lane2(20μg), MCF-7 (lane3(20μg), C2C12 (lane4(20μg) and C6 (lane5(20μg) using Bax Antibody (HY-P80027). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bax Antibody (YA825)
    Immunohistochemical analysis of paraffin-embedded endometrial cancer tissue using Bax Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80027, 1/100) , competitor's antibody (1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Bax Antibody (YA825)
    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using BAX Antibody (YA825). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80027, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Bax Antibody (YA825)
    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using BAX Antibody (YA825). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80027, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

  • Function

    Plays a role in the mitochondrial apoptotic process (PubMed:10772918, PubMed:11060313, PubMed:16113678, PubMed:16199525, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:36361894, PubMed:8358790, PubMed:8521816). Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed:21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis (PubMed:10772918, PubMed:11060313, PubMed:16113678, PubMed:16199525, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:8358790, PubMed:8521816). Promotes activation of CASP3, and thereby apoptosis (PubMed:10772918, PubMed:11060313, PubMed:16113678, PubMed:16199525, PubMed:18948948, PubMed:21199865, PubMed:21458670, PubMed:25609812, PubMed:8358790, PubMed:8521816)

  • Subcellular Localization

    Mitochondrion outer membrane; Single-pass membrane protein; Cytoplasm; Nucleus; Cytoplasm; Cytoplasm; Cytoplasm

  • Expression


    Tissue_specificity:This gene is expressed in a variety of tissues. The Psi isoform is present in gliomas. The α isoform is expressed in the spleen, breast, ovary, testis, colon, and brain, and at low levels in the skin and lungs. The Sigma isoform is expressed in the spleen, breast, ovary, testis, lung, colon, and brain, and at low levels in the skin. Both the α and Sigma isoforms are expressed in promyelocytic leukemia, histiocytic lymphoma, Burkitt lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovarian adenocarcinoma, prostate cancer, prostate adenocarcinoma, lung cancer, epidermoid carcinoma, small cell lung cancer, and colon adenocarcinoma cell lines.

  • Subunit

    Homodimer. Forms higher oligomers under stress conditions. Forms heterooligomers with BAK (PubMed:29531808). Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1 (PubMed:25609812). Interacts with SH3GLB1. Interacts with humanin; forms fibers with humanin which results in BAX conformational changes and sequestering of BAX into the fibers, preventing BAX activation (PubMed:12732850, PubMed:31690630). Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells. Interacts with FAIM2/LFG2. Interacts with RTL10/BOP. Interacts (via a C-terminal 33 residues) with NOL3 (via CARD domain); inhibits BAX activation and translocation and consequently cytochrome c release from mitochondria. Interacts with GIMAP3/IAN4 and GIMAP5/IAN5; this interaction is increased, when cells initiate apoptosis upon IL2 withdrawal (PubMed:16509771). Interacts with IRF3; the interaction is direct, increases upon Sendai virus infection and mediates the formation of the apoptosis complex TOMM70:HSP90AA1:IRF3:BAX (PubMed:25609812). Interacts with MOAP1, facilitating BAX-dependent mitochondrial outer membrane permeabilization and apoptosis (PubMed:11060313, PubMed:16199525). Interacts with BCL2L10/BCL-B (PubMed:23235460). Interacts with non-acetylated XRCC6/Ku70; this interaction leads to BAX sequestration in the cytosol, away from the mitochondria, preventing BAX-mediated apoptosis (PubMed:15023334)

  • SwissProt ID

    Q07812

  • Gene ID
    581 [NCBI]
  • Synonyms

    Apoptosis regulator BAX; BAX; Bcl-2-like protein 4; BCL2-associated X protein; Bcl2-L-4; BCL2L4

  • Research Field

    Cell Biology

Bax Antibody (YA825) Related Classifications

MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P80027, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody (HY-P80027) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), MCF-7 (lane3(20μg), C2C12 (lane4(20μg) and C6 (lane5(20μg) using Bax Antibody (HY-P80027). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded endometrial cancer tissue using Bax Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80027, 1/100) , competitor's antibody (1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using BAX Antibody (YA825). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80027, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • 1/16

    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using BAX Antibody (YA825). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80027, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Bax Antibody (YA825) Product Image
    1/16
  • Experimental Validation Results for Bax Antibody (YA825)
  • Experimental Validation Results for Bax Antibody (YA825)
  • Experimental Validation Results for Bax Antibody (YA825)
  • Experimental Validation Results for Bax Antibody (YA825)
  • Experimental Validation Results for Bax Antibody (YA825)
  • Experimental Validation Results for Bax Antibody (YA825)
  • Bax Antibody (YA825) Product Image
  • Experimental Validation Results for Bax Antibody (YA825)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P80027, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bax Antibody (YA825)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody (HY-P80027) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bax Antibody (YA825)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Hela (lane2(20μg), MCF-7 (lane3(20μg), C2C12 (lane4(20μg) and C6 (lane5(20μg) using Bax Antibody (HY-P80027). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Bax Antibody (YA825)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded endometrial cancer tissue using Bax Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80027, 1/100) , competitor's antibody (1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.
  • Experimental Validation Results for Bax Antibody (YA825)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using BAX Antibody (YA825). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80027, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Bax Antibody (YA825)
    1/1
    MedchemExpress Validation
     Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using BAX Antibody (YA825). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80027, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Experimental Validation Results for Bax Antibody (YA825)
  • Experimental Validation Results for Bax Antibody (YA825)
  • Experimental Validation Results for Bax Antibody (YA825)
  • Experimental Validation Results for Bax Antibody (YA825)
  • Experimental Validation Results for Bax Antibody (YA825)
  • Experimental Validation Results for Bax Antibody (YA825)
  • Bax Antibody (YA825) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (HY-P80027, 1/1000) , competitor's antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Bax Antibody (YA825) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from HeLa (lane 1(20μg)) 、HepG2 (lane 2(20μg)) 、A549 (lane 3(20μg)) 、C2C12 (lane 4(20μg)) 、C6 (lane 5(20μg)) 、HEK293 (lane 6(20μg)) 、Mouse kidney (lane 7(20μg)) 、Rat kidney (lane 8(20μg)) and Mouse spleen (lane 9(20μg)) using Bax Antibody (HY-P80027) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Hsp90, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Bax Antibody (YA825) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Hela (lane2(20μg), MCF-7 (lane3(20μg), C2C12 (lane4(20μg) and C6 (lane5(20μg) using Bax Antibody (HY-P80027). Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80993, 1/10,000) was used in 5% non-fat milk in TBST for 2 hour at room temperature. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • Bax Antibody (YA825) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded endometrial cancer tissue using Bax Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH 9.0) for 20 minutes. The tissues were probed with the primary antibody (HY-P80027, 1/100) , competitor's antibody (1/100) overnight at 4℃. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with neutral balsam.

  • Bax Antibody (YA825) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using BAX Antibody (YA825). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80027, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Bax Antibody (YA825) Product Image
    MedchemExpress Validation

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using BAX Antibody (YA825). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80027, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Bax Antibody (YA825) Product Image WB
  • Bax Antibody (YA825) Product Image WB
  • Bax Antibody (YA825) Product Image WB
  • Bax Antibody (YA825) Product Image IHC
  • Bax Antibody (YA825) Product Image IHC-P
  • Bax Antibody (YA825) Product Image IHC-P