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UCHL5 Antibody (YA2339)(PBS only)

(Synonyms: UCH37; CGI-70; INO80R; UCH-L5)

UCHL5 Antibody (YA2339) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to UCHL5.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF, IP

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS, pH 7.4.

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for UCHL5 Antibody (YA2339)(PBS only)
  • Experimental Validation Results for UCHL5 Antibody (YA2339)(PBS only)
  • Experimental Validation Results for UCHL5 Antibody (YA2339)(PBS only)
  • UCHL5 Antibody (YA2339)(PBS only) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:1000 1:50-1:200 1:20

Product Details

Description

UCHL5 Antibody (YA2339) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to UCHL5.

  • Host Rabbit
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 38 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 38 kDa
Immunogen

A synthetic peptide of human UCH37

Purification

Affinity Purified

Conjugation

Non-conjugated

Isotype

IgG

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS, pH 7.4.

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for UCHL5 Antibody (YA2339)(PBS only)
    Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , HEK293T(lane 3(20ug) and Jurkat(lane 4(20ug) using UCHL5 Antibody (HY-P82594) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for UCHL5 Antibody (YA2339)(PBS only)
    Immunocytochemistry analysis of SH-SY5Y cells labeling UCHL5 with UCHL5 Antibody (HY-P82594) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with UCHL5 Antibody (HY-P82594) at 1/50 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for UCHL5 Antibody (YA2339)(PBS only)
    Immunocytochemistry analysis of SH-SY5Y cells labeling UCHL5 with UCHL5 Antibody (HY-P82594) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with UCHL5 Antibody (HY-P82594) at 1/100 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    UCHL5 is a Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Isoforms & Post-Translational Modification

    Q9Y5K5 has 4 isomers: Q9Y5K5-1: 37607 Da (predicted); Q9Y5K5-2: 36079 Da (predicted); Q9Y5K5-3: 37478 Da (predicted); Q9Y5K5-4: 37353 Da (predicted).

  • Subunit

    Component of the 19S (PA700) regulatory complex of the 26S proteasome. Interacts with ADRM1 and NFRKB; in vitro ADRM1 and NFRKB compete for interaction with UCHL5. Component of the INO80 complex; specifically part of a complex module associated with N-terminus of INO80

  • SwissProt ID

    Q9Y5K5

  • Gene ID
    51377 [NCBI]
  • Synonyms

    UCH37; CGI-70; INO80R; UCH-L5

  • Research Field

    Cell Biology

UCHL5 Antibody (YA2339)(PBS only) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , HEK293T(lane 3(20ug) and Jurkat(lane 4(20ug) using UCHL5 Antibody (HY-P82594) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of SH-SY5Y cells labeling UCHL5 with UCHL5 Antibody (HY-P82594) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with UCHL5 Antibody (HY-P82594) at 1/50 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of SH-SY5Y cells labeling UCHL5 with UCHL5 Antibody (HY-P82594) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with UCHL5 Antibody (HY-P82594) at 1/100 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • UCHL5 Antibody (YA2339)(PBS only) Product Image
    1/16
  • Experimental Validation Results for UCHL5 Antibody (YA2339)(PBS only)
  • Experimental Validation Results for UCHL5 Antibody (YA2339)(PBS only)
  • Experimental Validation Results for UCHL5 Antibody (YA2339)(PBS only)
  • UCHL5 Antibody (YA2339)(PBS only) Product Image
  • Experimental Validation Results for UCHL5 Antibody (YA2339)(PBS only)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , HEK293T(lane 3(20ug) and Jurkat(lane 4(20ug) using UCHL5 Antibody (HY-P82594) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Experimental Validation Results for UCHL5 Antibody (YA2339)(PBS only)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of SH-SY5Y cells labeling UCHL5 with UCHL5 Antibody (HY-P82594) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with UCHL5 Antibody (HY-P82594) at 1/50 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for UCHL5 Antibody (YA2339)(PBS only)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of SH-SY5Y cells labeling UCHL5 with UCHL5 Antibody (HY-P82594) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with UCHL5 Antibody (HY-P82594) at 1/100 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for UCHL5 Antibody (YA2339)(PBS only)
  • Experimental Validation Results for UCHL5 Antibody (YA2339)(PBS only)
  • Experimental Validation Results for UCHL5 Antibody (YA2339)(PBS only)
  • UCHL5 Antibody (YA2339)(PBS only) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , HEK293T(lane 3(20ug) and Jurkat(lane 4(20ug) using UCHL5 Antibody (HY-P82594) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • UCHL5 Antibody (YA2339)(PBS only) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of SH-SY5Y cells labeling UCHL5 with UCHL5 Antibody (HY-P82594) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with UCHL5 Antibody (HY-P82594) at 1/50 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • UCHL5 Antibody (YA2339)(PBS only) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of SH-SY5Y cells labeling UCHL5 with UCHL5 Antibody (HY-P82594) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with UCHL5 Antibody (HY-P82594) at 1/100 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • UCHL5 Antibody (YA2339)(PBS only) Product Image WB
  • UCHL5 Antibody (YA2339)(PBS only) Product Image ICC
  • UCHL5 Antibody (YA2339)(PBS only) Product Image ICC