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  5. UCHL5 Antibody (YA2339)

UCHL5 Antibody (YA2339) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to UCHL5.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • Description

Description

UCHL5 Antibody (YA2339) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to UCHL5.
Host

Rabbit
Clonality

Recombinant,Monoclonal
Masse moléculaire
Predicted band size: 38 kDa;
Observed band size: 38 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

A synthetic peptide of human UCH37
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:50-1:200
IP
IP: Immunoprecipitation
1:20
Sensitivity Endogenous Pureté Affinity Purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 10mM PBS, pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Livraison

Shipping with blue ice.
Verification Image
ALL WB ICC

  • Western blot analysis of extracts from NIH/3T3(lane 2(20ug) , HEK293T(lane 3(20ug) and Jurkat(lane 4(20ug) using UCHL5 Antibody (HY-P82594) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of SH-SY5Y cells labeling UCHL5 with UCHL5 Antibody (HY-P82594) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with UCHL5 Antibody (HY-P82594) at 1/50 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of SH-SY5Y cells labeling UCHL5 with UCHL5 Antibody (HY-P82594) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with UCHL5 Antibody (HY-P82594) at 1/100 dilution in BSA for Immunol Staining the night at 4 ℃. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background
Function：Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1
Subcellular Localization：Cytoplasm; Nucleus
Isoforms & Post-Translational Modification：Q9Y5K5 has 4 isomers: Q9Y5K5-1: 37607 Da (predicted); Q9Y5K5-2: 36079 Da (predicted); Q9Y5K5-3: 37478 Da (predicted); Q9Y5K5-4: 37353 Da (predicted).
Subunit：Component of the 19S (PA700) regulatory complex of the 26S proteasome. Interacts with ADRM1 and NFRKB; in vitro ADRM1 and NFRKB compete for interaction with UCHL5. Component of the INO80 complex; specifically part of a complex module associated with N-terminus of INO80
RRID
Database

Entrez Gene: 51377 Human ; 56207 Mouse ; 360853 Rat

SwissProt: Q9Y5K5 Human ; Q9WUP7 Mouse ;

OMIM: 610667 Human

Research Field

Cell Biology
Synonyms
UCH37; CGI-70; INO80R; UCH-L5
Documentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

UCHL5 Antibody (YA2339) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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UCHL5 Antibody (YA2339)
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HY-P82594
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SAFETY DATA SHEET (SDS)

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