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Acetyl-Histone H4 (Lys8) Antibody (YA7589)

(Synonyms: H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4, H4K5ac)
Customer Review

Based on 1 Customer Validation

Acetyl-Histone H4 (Lys8) Antibody (YA7589) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Acetyl-Histone H4 (Lys8).

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-P, ICC/IF, IP, ELISA

  • Reactivity :

    Human, Mouse, Rat

  • Formulation:

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Acetyl-Histone H4 (Lys8) Antibody (YA7589)
  • Acetyl-Histone H4 (Lys8) Antibody (YA7589) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
IP Info
IP: Immunoprecipitation
Dilution Ratio 1:500-1:2000 1:2000-1:10000 1:200-1:1000 1:5000-1:20000 1:50-1:200

Product Details

Description

Acetyl-Histone H4 (Lys8) Antibody (YA7589) is a Rabbit-derived and non-conjugated IgG, Kappa monoclonal antibody, targeting to Acetyl-Histone H4 (Lys8).

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Human, Mouse, Rat
  • Observed Molecular Weight
    Observed band size: 11 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 11 kDa
Immunogen

The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

Protein A affinity purified

Conjugation

Non-conjugated

Modification

Acetylated

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4) containing 50% glycerol, 0.05% Proclin 300, 0.05%BSA

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Acetyl-Histone H4 (Lys8) Antibody (YA7589)
    Western blot analysis of extracts from NIH-3T3 (lane 1(20μg)) 、NIH-3T3+TSA (lane 2(20μg)) 、C6 (lane 3(20μg)) 、C6+TSA (lane 4(20μg)) using Histone H4 (Acetyl Lys8) Antibody (HY-P87904) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Acetyl-Histone H4 core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling

  • Subcellular Localization

    Nucleus,Chromosome

  • Isoforms & Post-Translational Modification

    P62805: 103 amino acids, molecular weight 11367 Da.

  • Subunit

    The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers

  • SwissProt ID

    P62805

  • Synonyms

    H4/A, H4FA, HIST1H4A, H4C2, H4/I, H4FI, HIST1H4B, H4C3, H4/G, H4FG, HIST1H4C, H4C4, H4/B, H4FB, HIST1H4D, H4C5, H4/J, H4FJ, HIST1H4E, H4C6, H4/C, H4FC, HIST1H4F, H4C8, H4/H, H4FH, HIST1H4H, H4C9, H4/M, H4FM, HIST1H4I, H4C11, H4/E, H4FE, HIST1H4J, H4C12, H4/D, H4FD, HIST1H4K, H4C13, H4/K, H4FK, HIST1H4L, H4C14, H4/N, H4F2, H4FN, HIST2H4, HIST2H4A, H4C15, H4/O, H4FO, HIST2H4B, H4C16, H4-16, HIST4H4, H4C1, Histone H4, H4K5ac

Acetyl-Histone H4 (Lys8) Antibody (YA7589) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from NIH-3T3 (lane 1(20μg)) 、NIH-3T3+TSA (lane 2(20μg)) 、C6 (lane 3(20μg)) 、C6+TSA (lane 4(20μg)) using Histone H4 (Acetyl Lys8) Antibody (HY-P87904) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Acetyl-Histone H4 (Lys8) Antibody (YA7589) Product Image
    1/16
  • Experimental Validation Results for Acetyl-Histone H4 (Lys8) Antibody (YA7589)
  • Acetyl-Histone H4 (Lys8) Antibody (YA7589) Product Image
  • Experimental Validation Results for Acetyl-Histone H4 (Lys8) Antibody (YA7589)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from NIH-3T3 (lane 1(20μg)) 、NIH-3T3+TSA (lane 2(20μg)) 、C6 (lane 3(20μg)) 、C6+TSA (lane 4(20μg)) using Histone H4 (Acetyl Lys8) Antibody (HY-P87904) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for Acetyl-Histone H4 (Lys8) Antibody (YA7589)
  • Acetyl-Histone H4 (Lys8) Antibody (YA7589) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from NIH-3T3 (lane 1(20μg)) 、NIH-3T3+TSA (lane 2(20μg)) 、C6 (lane 3(20μg)) 、C6+TSA (lane 4(20μg)) using Histone H4 (Acetyl Lys8) Antibody (HY-P87904) . Proteins were transferred to a PVDF membrane and blocked with 5% nonfat dry milk in TBST for 1.5 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (β Actin, 1/10000) was used in 5% nonfat dry milk in TBST at 4℃ overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/10,000) was used for 1 hour at room temperature.

  • Acetyl-Histone H4 (Lys8) Antibody (YA7589) Product Image WB