AMSH Antibody (YA2707)

Cat. No.: HY-P82962: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

AMSH Antibody (YA2707) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AMSH.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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AMSH Antibody (YA2707) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

AMSH Antibody (YA2707) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to AMSH.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 48 kDa;

Observed band size: 48 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O95630

Gene ID

10617 [NCBI]

Immunogen

A synthetic peptide of human AMSH

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from A549(lane 2(20ug) ，HL-60(lane 3(20ug) and HepG2(lane 4(20ug) using AMSH Antibody (HY-P82962) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains (PubMed:15314065, PubMed:23542699, PubMed:34425109). Does not cleave 'Lys-48'-linked polyubiquitin chains (PubMed:15314065). Plays a role in signal transduction for cell growth and MYC induction mediated by IL-2 and GM-CSF (PubMed:10383417). Potentiates BMP (bone morphogenetic protein) signaling by antagonizing the inhibitory action of SMAD6 and SMAD7 (PubMed:11483516). Has a key role in regulation of cell surface receptor-mediated endocytosis and ubiquitin-dependent sorting of receptors to lysosomes (PubMed:15314065, PubMed:17261583). Endosomal localization of STAMBP is required for efficient EGFR degradation but not for its internalization (PubMed:15314065, PubMed:17261583). Involved in the negative regulation of PI3K-AKT-mTOR and RAS-MAP signaling pathways (PubMed:23542699)

Subcellular Localization：Nucleus; Membrane; Peripheral membrane protein; Cytoplasm; Early endosome

Expression：
Tissue_specificity:Ubiquitously expressed

Isoforms & Post-Translational Modification：O95630 has 2 isomers: O95630-1: 48077 Da (predicted); O95630-2: 39596 Da (predicted).
Phosphorylated after BMP type I receptor activation;Ubiquitinated by SMURF2 in the presence of RNF11

Subunit：Interacts with STAM (PubMed:10383417, PubMed:15314065). Interacts with SMAD6 and SMAD7 (PubMed:11483516). Interacts with CHMP3; the interaction appears to relieve the autoinhibition of CHMP3 (PubMed:17146056, PubMed:17261583).

RRID

AB_3105081

Database

Entrez Gene: 10617 Human

SwissProt: O95630 Human

OMIM: 614261 Human

Research Field

Cell Biology

Synonyms

AMSH; MICCAP

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

AMSH Antibody (YA2707) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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AMSH Antibody (YA2707)

AMSH Antibody (YA2707) Featured Recommendations:

Product Detail

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Background

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AMSH Antibody (YA2707) Related Classifications

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