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M-CSF Antibody (YA301)

(Synonyms: CSF1; Macrophage colony-stimulating factor 1; CSF-1; M-CSF; MCSF; Lanimostim)
Customer Review

Based on 1 Customer Validation

M-CSF Antibody (YA301) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to M-CSF.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for M-CSF Antibody (YA301)
  • Experimental Validation Results for M-CSF Antibody (YA301)
  • M-CSF Antibody (YA301) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200

Product Details

Description

M-CSF Antibody (YA301) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to M-CSF.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 60 kDa
Species Reactivity Database

Entrez Gene: 1435 Human

SwissProt: P09603 Human

Immunogen

Synthetic peptide corresponding to Human M-CSF.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102692

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for M-CSF Antibody (YA301)
    Western blot analysis of extracts from Jurkat(lane 2(20μg) or lane 3(40μg) ) and K562(lane 4(20μg) or lane 5(40μg)) using M-CSF(HY-P80750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80750, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for M-CSF Antibody (YA301)
    Western blot analysis was performed on protein extracts (25 μg) from Jurkat (lane 2, Negative), K562 (lane 3), Caco-2 (lane 4), THP-1 (lane 5), 293T (lane 6), and HeLa (lane 7) using MCS-F antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Secreted, extracellular space

  • Isoforms & Post-Translational Modification

    P09603 has 3 isomers: P09603-1: 60179 Da (predicted); P09603-2: 47903 Da (predicted); P09603-3: 29215 Da (predicted).
    N-glycosylated;N-glycosylated;N-glycosylated;O-glycosylated; contains chondroitin sulfate (PubMed:1531650, PubMed:8051056). O-glycosylated with core 1 or possibly core 8 glycans (PubMed:22171320, PubMed:23234360, PubMed:3264877);O-glycosylated

  • Subunit

    Homodimer or heterodimer; disulfide-linked (PubMed:1531650, PubMed:3264877, PubMed:8422357). Likely to exist in multiple forms: homodimer consisting of 2 identical 150-200 kDa proteoglycan subunits, heterodimer consisting of a 150-200 kDa proteoglycan subunit and a truncated 43 kDa subunit, and homodimer consisting of 2 identical 43 kDa subunits (PubMed:8051056). Interacts with CSF1R (PubMed:20504948)

  • SwissProt ID

    P09603

  • Gene ID
    1435 [NCBI]
  • Synonyms

    CSF1; Macrophage colony-stimulating factor 1; CSF-1; M-CSF; MCSF; Lanimostim

  • Research Field

    Immunology

M-CSF Antibody (YA301) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20μg) or lane 3(40μg) ) and K562(lane 4(20μg) or lane 5(40μg)) using M-CSF(HY-P80750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80750, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Jurkat (lane 2, Negative), K562 (lane 3), Caco-2 (lane 4), THP-1 (lane 5), 293T (lane 6), and HeLa (lane 7) using MCS-F antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • M-CSF Antibody (YA301) Product Image
    1/16
  • Experimental Validation Results for M-CSF Antibody (YA301)
  • Experimental Validation Results for M-CSF Antibody (YA301)
  • M-CSF Antibody (YA301) Product Image
  • Experimental Validation Results for M-CSF Antibody (YA301)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20μg) or lane 3(40μg) ) and K562(lane 4(20μg) or lane 5(40μg)) using M-CSF(HY-P80750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80750, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for M-CSF Antibody (YA301)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Jurkat (lane 2, Negative), K562 (lane 3), Caco-2 (lane 4), THP-1 (lane 5), 293T (lane 6), and HeLa (lane 7) using MCS-F antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for M-CSF Antibody (YA301)
  • Experimental Validation Results for M-CSF Antibody (YA301)
  • M-CSF Antibody (YA301) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Jurkat(lane 2(20μg) or lane 3(40μg) ) and K562(lane 4(20μg) or lane 5(40μg)) using M-CSF(HY-P80750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80750, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • M-CSF Antibody (YA301) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from Jurkat (lane 2, Negative), K562 (lane 3), Caco-2 (lane 4), THP-1 (lane 5), 293T (lane 6), and HeLa (lane 7) using MCS-F antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • M-CSF Antibody (YA301) Product Image WB
  • M-CSF Antibody (YA301) Product Image WB