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M-CSF Antibody (YA301)

(Synonyms: CSF1; Macrophage colony-stimulating factor 1; CSF-1; M-CSF; MCSF; Lanimostim)
Customer Review

Based on 1 Customer Validation

M-CSF Antibody (YA301) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to M-CSF.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, IHC-F, IHC-P, ICC/IF

  • Reactivity :

    Human

  • Formulation:

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Conjugation:
    Non-conjugated
ALL WB ICC
  • Experimental Validation Results for M-CSF Antibody (YA301)
  • Experimental Validation Results for M-CSF Antibody (YA301)
  • Experimental Validation Results for M-CSF Antibody (YA301)
  • Experimental Validation Results for M-CSF Antibody (YA301)
  • M-CSF Antibody (YA301) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
IHC-F Info
IHC-F: Immunohistochemistry-Frozen
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:500-1:1000 1:50-1:100 1:50-1:100 1:50-1:200

Product Details

Description

M-CSF Antibody (YA301) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to M-CSF.

  • Host Rabbit
  • Clonality Recombinant,Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 43 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 60 kDa
Species Reactivity Database

Entrez Gene: 1435 Human

SwissProt: P09603 Human

Immunogen

Synthetic peptide corresponding to Human M-CSF.The exact sequence is proprietary to MCE.

Sensitivity

Endogenous

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

RRID

AB_3102692

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for M-CSF Antibody (YA301)
    Western blot analysis was performed on protein extracts (25 μg) from Jurkat (lane 2, Negative), K562 (lane 3), Caco-2 (lane 4), THP-1 (lane 5), 293T (lane 6), and HeLa (lane 7) using MCS-F antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for M-CSF Antibody (YA301)
    Immunocytochemistry analysis of Hela cells labeling M-CSF with M-CSF Antibody (HY-P80750) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with M-CSF Antibody (HY-P80750) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for M-CSF Antibody (YA301)
    Immunocytochemistry analysis of Hela cells labeling M-CSF with M-CSF Antibody (HY-P80750) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with M-CSF Antibody (HY-P80750) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for M-CSF Antibody (YA301)
    Western blot analysis of extracts from Jurkat(lane 2(20μg) or lane 3(40μg) ) and K562(lane 4(20μg) or lane 5(40μg)) using M-CSF(HY-P80750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80750, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

  • Function

    Macrophage colony-stimulating factor (M-CSF), also known as colony-stimulating factor-1 (CSF-1), is a hemopoietic growth factor that regulates the survival, proliferation, differentiation and function of the cells of the mononuclear phagocyte lineage. M-C

  • Subcellular Localization

    Cell membrane; Single-pass type I membrane protein; Secreted, extracellular space

  • Isoforms & Post-Translational Modification

    P09603 has 3 isomers: P09603-1: 60179 Da (predicted); P09603-2: 47903 Da (predicted); P09603-3: 29215 Da (predicted).
    N-glycosylated;N-glycosylated;N-glycosylated;O-glycosylated; contains chondroitin sulfate (PubMed:1531650, PubMed:8051056). O-glycosylated with core 1 or possibly core 8 glycans (PubMed:22171320, PubMed:23234360, PubMed:3264877);O-glycosylated

  • Subunit

    Homodimer or heterodimer; disulfide-linked (PubMed:1531650, PubMed:3264877, PubMed:8422357). Likely to exist in multiple forms: homodimer consisting of 2 identical 150-200 kDa proteoglycan subunits, heterodimer consisting of a 150-200 kDa proteoglycan subunit and a truncated 43 kDa subunit, and homodimer consisting of 2 identical 43 kDa subunits (PubMed:8051056). Interacts with CSF1R (PubMed:20504948)

  • SwissProt ID

    P09603

  • Gene ID
    1435 [NCBI]
  • Synonyms

    CSF1; Macrophage colony-stimulating factor 1; CSF-1; M-CSF; MCSF; Lanimostim

  • Research Field

    Immunology

M-CSF Antibody (YA301) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Jurkat (lane 2, Negative), K562 (lane 3), Caco-2 (lane 4), THP-1 (lane 5), 293T (lane 6), and HeLa (lane 7) using MCS-F antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling M-CSF with M-CSF Antibody (HY-P80750) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with M-CSF Antibody (HY-P80750) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling M-CSF with M-CSF Antibody (HY-P80750) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with M-CSF Antibody (HY-P80750) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20μg) or lane 3(40μg) ) and K562(lane 4(20μg) or lane 5(40μg)) using M-CSF(HY-P80750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80750, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • M-CSF Antibody (YA301) Product Image
    1/16
  • Experimental Validation Results for M-CSF Antibody (YA301)
  • Experimental Validation Results for M-CSF Antibody (YA301)
  • Experimental Validation Results for M-CSF Antibody (YA301)
  • Experimental Validation Results for M-CSF Antibody (YA301)
  • M-CSF Antibody (YA301) Product Image
  • Experimental Validation Results for M-CSF Antibody (YA301)
    1/1
    MedChemExpress Validation
     Western blot analysis was performed on protein extracts (25 μg) from Jurkat (lane 2, Negative), K562 (lane 3), Caco-2 (lane 4), THP-1 (lane 5), 293T (lane 6), and HeLa (lane 7) using MCS-F antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for M-CSF Antibody (YA301)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling M-CSF with M-CSF Antibody (HY-P80750) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with M-CSF Antibody (HY-P80750) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for M-CSF Antibody (YA301)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of Hela cells labeling M-CSF with M-CSF Antibody (HY-P80750) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with M-CSF Antibody (HY-P80750) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for M-CSF Antibody (YA301)
    1/1
    MedchemExpress Validation
     Western blot analysis of extracts from Jurkat(lane 2(20μg) or lane 3(40μg) ) and K562(lane 4(20μg) or lane 5(40μg)) using M-CSF(HY-P80750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80750, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.
  • Experimental Validation Results for M-CSF Antibody (YA301)
  • Experimental Validation Results for M-CSF Antibody (YA301)
  • Experimental Validation Results for M-CSF Antibody (YA301)
  • Experimental Validation Results for M-CSF Antibody (YA301)
  • M-CSF Antibody (YA301) Product Image
    MedChemExpress Validation

    Western blot analysis was performed on protein extracts (25 μg) from Jurkat (lane 2, Negative), K562 (lane 3), Caco-2 (lane 4), THP-1 (lane 5), 293T (lane 6), and HeLa (lane 7) using MCS-F antibody. Proteins were transferred onto a 0.45 μm PVDF membrane using the Trans-Blot® Turbo™ system for 13 min. The membrane was then blocked with 5% nonfat milk in TBST (HY-K1025) for 1 h at room temperature. The primary antibody (1:1000) and loading control antibody GAPDH Antibody (HRP) (HY-P80954A) (1:5000) were diluted in 5% nonfat milk in TBST and incubated with the membrane overnight at 4°C. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • M-CSF Antibody (YA301) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling M-CSF with M-CSF Antibody (HY-P80750) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with M-CSF Antibody (HY-P80750) at 1/100 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • M-CSF Antibody (YA301) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of Hela cells labeling M-CSF with M-CSF Antibody (HY-P80750) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with M-CSF Antibody (HY-P80750) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Rabbit IgG H&L (HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • M-CSF Antibody (YA301) Product Image
    MedchemExpress Validation

    Western blot analysis of extracts from Jurkat(lane 2(20μg) or lane 3(40μg) ) and K562(lane 4(20μg) or lane 5(40μg)) using M-CSF(HY-P80750) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80750, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Rabbit/Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

  • M-CSF Antibody (YA301) Product Image WB
  • M-CSF Antibody (YA301) Product Image ICC
  • M-CSF Antibody (YA301) Product Image ICC
  • M-CSF Antibody (YA301) Product Image WB